Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 34 for “"protein transport"”.
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Structural Studies of Protein Transport
Most new proteins are made in the cytosol and then targeted to their mature destinations. In the case of integral membrane proteins (IMPs), this targeting concludes with integration into the target membrane, a process typically facilitated by protein-conducting channels. This thesis examines the …
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The Role of HSP90C in Chloroplast Maturation and Thylakoid Protein Transport
… in green plants and contains thousands of proteins. Since most chloroplast proteins are synthesized outside of the chloroplast on cytosolic ribosomes, they are imported into chloroplasts in mostly unfolded states. A protein quality control system in the chloroplast is then responsible for …
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Regulation of Polarized Protein Transport to Axons, Dendrites, and Sensory Cilia in Caeborhabditis Elegans Neurons
… receptors, synaptic vesicles, and signaling proteins. Yet, despite the significance of asymmetric protein transport in neuronal polarity, the molecules and mechanisms that direct polarized transport to axons, dendrites and cilia in neurons are only partly understood. In this thesis, I …
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Interactions between the TatBC complex and Tat signal peptides during protein transport by the bacterial Tat pathway
… Sec pathways operate in parallel to translocate proteins across the prokaryotic cytoplasmic membrane and the thylakoid membrane of plant chloroplasts. Unlike the Sec pathway, which translocates unfolded proteins, substrates of the Tat system are transported in a folded state. In Escherichia coli, …
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Protein polarization in packed hollow fibre bioreactors
Osmotically active proteins and cells, retained in the extra capillary space (ECS) of hollow fibre bioreactors (HFBRs), can influence the hydrodynamics of such devices. A mathematical model was developed to describe the coupled hydrodynamics and high molecular weight protein transport in a cell …
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Studies of intraorganelle dynamics: the lysosome, the pre-lysosomal compartment, and the golgi apparatus
… dynamics. Lysosomal contents and membrane proteins were shown to intermix rapidly in fused mammalian cells, with a t<sub>½</sub> of ~30 min. Lysosomal content intermixing, shown by a sensitive invertase-lysosome/[¹⁴C]-sucrose-lysosome pairing assay, was inhibited greatly by ATP inhibitors …
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Local Protein Turnover As a Regulatory Mechanism of Growth and Collapse of Neuronal Growth Cones
… Wachstumskegel Oberflächenrezeptoren und Signalproteine welche den Umbau von Strukturproteinen, zum Beispiel Aktin, Tubulin, Spektrin oder Neurofilament, beeinflussen. Angesichts der grossen Distanz zwischen Wachtumskegel und Zellkörper sind lokale Möglichkeiten zur Regulation der Proteinmengen …
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Systems Biology Approaches for Elucidating Early ALS Disease Processes
… for extracellular matrix organization and protein transport. Integrating these findings with results from a C9orf72 Drosophila model, I found causal and compensatory pathways that may be active in C9-ALS. Next, I investigated the genetics of ALS using genomic, transcriptomic, and epigenomic …
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Super Low Dose Endotoxin Exacerbates Low Grade Inflammation through Modulating Cell Stress and Decreasing Cellular Homeostatic Protein Expression
… resulted in the phosphorylation of Golgi protein GRASP, leading to unstacking of Golgi cisterna and overall dysfunction of the Golgi apparatus. Dysfunctional Golgi apparatus and its effect on protein transport and secretion, may account for decreased levels of Site 2 Protease, reduced …
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Identifizierung von Mikrodomän-Proteinen und funktionelle Charakterisierung von Cholesterin im Golgi-Apparat
… Mikrodomänen isoliert, die zehn Proteine entsprechend der Coomassiefärbung in der SDS-PAGE enthalten. Im Rahmen dieser Arbeit wurden die zwei bisher nicht charakterisierten Proteine mit Molekulargewichten von 72 und 56 kDa als Untereinheiten A und B der vakuolären ATPase …
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Multi-Step Trafficking Pathway for Regulation of the Protein Composition of the Chlamydomonas Flagellar Membrane during Cilium-Generated Signaling
… compartment for membrane and cytoplasmic proteins that mediate multiple sensory processes. Little is known about the cellular and molecular mechanisms that determine regulated ciliary localization of membrane or soluble proteins. Our laboratory uses the biflagellated green alga …
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Influence of Cellular Localization on Activity of Hydroxysteroid Dehydrogenases
Hydroxysteroid dehydrogenases (HSDs) catalyze the interconversion of inactive steroids and active hormones. HSDs use nicotinamide cofactors in the cytosol and endoplasmic reticulum (ER) lumen to either reduce or oxidize their steroid substrates. Our lab has extensively studied the 17beta-HSDs types …
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Study of the Cellular Role of GRP78/BiP mono-ADP-ribosylation in UPR and Cancer
… is a reversible post-translational protein modification that modulates the function of proteins involved in different cellular processes, including signal transduction, protein transport, transcription, cell cycle regulation, DNA (deoxyribonucleic acid) repair and apoptosis. In …
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A Glomerular Basement Membrane-on-a-Chip Model for Investigating Charge-Selective Transport Across the Human Filtration Barrier
… cascade and complement system, leading to protein adsorption on the membrane surface, impaired filtration, and adverse patient outcomes. This underscores the need for biocompatible membranes that better replicate the functional characteristics of the native glomerular filtration barrier. …
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Modeling the interface between biological and synthetic components in hybrid nanosystems
… used for detection of live bacteria; (2) protein transport through a nanochannel; (3) deriving the Langmuir constant for adsorption for small, organic molecules on synthetic surfaces; (4) creating a multiscale model for transport in micro- and nanofluidic devices; (5) synthetic analogs of …
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Protons and Potentials: How Neurotransmission Influences and is Influenced by pH Transients
… functions, contributing to energy metabolism, protein transport, protein modulation, and ion homeostasis. While usually tightly controlled by systemic and cellular processes, free hydrogen ions can overwhelm endogenous buffering during neurotransmission and can form transient increases or …
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The Characterization of Nemadipine and Migrazole as Small Molecule Tools for Use in the Nematode Caenorhabditis elegans
… may affect multiple pathways by perturbation of protein transport. To identify migrazole’s target, I performed a forward genetic screen for mutants resistant to migrazole’s effects. However, I was unable to identify the target of migrazole through analysis of the mutants I isolated. This result …
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Structural RNA Homology Search and Alignment Using Covariance Models
Functional RNA elements do not encode proteins, but rather function directly as RNAs. Many different types of RNAs play important roles in a wide range of cellular processes, including protein synthesis, gene regulation, protein transport, splicing, and more. Because important sequence and …
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Mapping protein-protein interactions in the <i>Escherichia coli</i> Twin Arginine Translocase
… Translocase (Tat) system is a membrane-bound transport system present in plants and bacteria that has the remarkable ability to export fully folded proteins across a lipid bilayer, powered by the protonmotive force. In Escherichia coli the Tat system is composed of only three essential …
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Characterization of the p23 interaction network
… to modulate conformations of native substrate proteins. By guiding substrates over potentially unstable intermediates, chaperones are able to shuttle clients through multiple functional forms to alter the composition and activity of complex protein assemblies. Thus, molecular chaperones …
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