Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"protein translocation"”.
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Computational investigation of protein translocation and membrane insertion
"The protein-conducting channel, or translocon, is an evolutionarily conserved complex which allows nascent proteins to cross a cellular membrane or integrate into it, typically in concert with a bound partner (e.g ., the ribosome). The crystal structure of an archaeal translocon, the SecY complex, …
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Fucntional Analysis of Sec61beta, a Component of the Sec61 protein Translocation Channel at the Endoplasmic Reticulum
… Pore die Translokation von sekretorischen Proteinen ins ER-Lumen sowie die Insertion von Membranproteinen in die ER-Membran. Der Komplex besteht aus einer a-Untereinheit, die den eigentlichen Translokationskanal bildet, sowie einer b- und einer g-Untereinheit, die mit dem Kanal assoziiert …
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Dynamic subcellular proteomics identifies novel regulators of adipocyte insulin action
… lipolysis. These processes rely on changes in protein localisation. For example, insulin signalling induces the translocation of the glucose transporter GLUT4 from an intracellular compartment to the plasma membrane. However, our knowledge of spatial changes in response to insulin signalling is …
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Biochemical and genetic analysis of a fission yeast ribonucleoprotein homologous to signal recognition particle
Protein translocation across the endoplasmic reticulum of higher cells is dependent on a ribonucleoprotein complex known as signal recognition particle (SRP). SRP is composed of six polypeptides and one molecule of 7SL RNA. A homolog of mammalian SRP RNA was found in the fission yeast, …
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Mechanistic studies of the ClpAP protease/
… chaperone, disassembling and remodeling its protein substrates. CIpA also serves as the ATPase component of the ClpAP protease, where it is resonsible for binding specific substrates and unfolding and translocating them into its partner ClpP, the proteolytic component of the complex. We have …
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A study of protein-DNA interactions using atomic force microscopy and DNA origami
… of the top concerns. Much of the detail of how proteins find their target sites and how they cleave at the sites remains unclear. Despite much progress in lab environments, where the tolerance for imprecise cutting is relatively high, in vivo treatment remains difficult. Most genome editing …
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Development of a cargo delivery system and inhibition studies focused on clostridium difficile toxin a
… exotoxins, disease arises from cytotoxic proteins, and cytotoxicity is acheived only after cell entry. This intrinsic mechanism for cell entry is intriguing from research and medical views. Along with a review on existing cargo delivery systems utilizing protein toxins and the usefulness …
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Isolation and characterization of Saccharomyces cerevisiae mutants for vacuolar import and autophagocytosis
The yeast vacuolar protein aminopeptidase 1 (Apelp) is synthesised as a cytosolic procursor that is transported to the vacuole via the cytoplasm to vacuole trnasport (Cvt) pathway. The cytosolic protein is enclosed in a double-membrane vesicle, which is transported to and fuses with the vacuole …
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Characterization of a Putative Acid Phosphatase in Toxoplasma Gondii and Its Role in Parasite Propagation
… cell, Toxoplasma secretes a large repertoire of proteins beyond the PV to manipulate important host cellular functions. How these Toxoplasma proteins transit from parasites to host cell is not well understood. Protein translocation into the host cell is mediated by three proteins hypothesized to …
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Parallel effects of signal peptide hydrophobic core modifications on cotranslational translocation and posttranslational cleavage by purified signal peptidase
Secretory proteins are targeted to the secretory pathway by an amino terminal extension sequence known as the signal peptide. Signal peptides contain a central core of uncharged amino acids that are usually hydrophobic in nature. The hydrophobic core of the parathyroid hormone (PTH) signal peptide …
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Interactions between the TatBC complex and Tat signal peptides during protein transport by the bacterial Tat pathway
… Sec pathways operate in parallel to translocate proteins across the prokaryotic cytoplasmic membrane and the thylakoid membrane of plant chloroplasts. Unlike the Sec pathway, which translocates unfolded proteins, substrates of the Tat system are transported in a folded state. In Escherichia coli, …
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Secretion of the chitinolytic machinery in Serratia marcescens
… substrate across the cell envelope in a single translocation event (one-step systems), and those that are dependent on the Sec or Tat machineries for export to the periplasm (two-step systems). Serratia marcescens is an important opportunistic human pathogen and has gathered a lot of interest …
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Characterization Of Streptolysin O As The Translocator For The Cytolysin-Mediated Translocation Process In Streptococcus Pyogenes
… pyogenes</italic> uses the pore-forming protein <underline>s</underline>trepto<underline>l</underline>ysin <underline>O</underline>: SLO) to translocate the <italic><underline>S.</underline></italic> <italic><underline>p</underline>yogenes</italic> …
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Identification and analysis of new phloem proteins from Brassicaceae and Cucurbitaceae
… work was the identification of new phloem sap proteins and a metabolic characterisation of this transport fluid. The experiments were performed on the three plant species C. sativus, C. maxima and B. napus. To characterise the phloem samples from B. napus, a new model plant for phloem analysis, …
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The Role of HSP90C in Chloroplast Maturation and Thylakoid Protein Transport
… in green plants and contains thousands of proteins. Since most chloroplast proteins are synthesized outside of the chloroplast on cytosolic ribosomes, they are imported into chloroplasts in mostly unfolded states. A protein quality control system in the chloroplast is then responsible for …
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The organization, expression, function and evolution of some essential genes from the hyperthermophilic eubacterium thermotoga maritima
… This fragment encodes five tRNAs, the ribosomal protein L33, an integral membrane protein, SecE, which is probably involved in protein translocation, the transcription factor NusG, four large subunit ribosomal proteins (L11, L1, L10 and L12), and the N-terminus of the RNA polymerase 0 subunit. …
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NUCLEAR ENVELOPE TRANSMEMBRANE PROTEIN DISTRIBUTION AND TRANSPORT STUDIED BY SINGLE-MOLECULE MICROSCOPY
… cytoplasm by forming a barrier. NE transmembrane proteins (NETs) embedded in either the outer nuclear membrane (ONM) or the inner nuclear membrane (INM) play crucial roles in both nuclear structure and functions, including: genome architecture, epigenetics, transcription, splicing, DNA …
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Annotation and comparative analysis of fungal genomes: a hitchhiker's guide to genomics
… plants; the tiny yeast P. pastoris is the major protein production platform in the pharmaceutical industry; the biosurfactant production yeast C. bombicola is likely to provide a low ecotoxicity detergent and E. huxleyi places in a unique phylogeny position of chromalveolate and contributes to …
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Microfluidic Engineering for Ultrasensitive Molecular Analysis of cells
… We demonstrate a simple protocol for detecting translocation of native proteins using a common flow cytometer which detects fluorescence intensity without imaging. Using our approach, we successfully detected the translocation of native NF-kappa B (an important transcription factor) at its …
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Members of the Preprotein and Amino Acid Transporter Family Constitute Components of Novel Protein Import Pathways into Chloroplasts
… plastid types must import the majority of their proteins from the cytosol across the envelope membranes. Translocons at the outer and inner chloroplast envelope membranes, called TOC and TIC, were identified that mediate the import of proteins. N-terminal transit peptides essential for import of …
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