Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 108 for “"protein translation"”.
-
Processes involved in protein translation
Protein translation—the process of converting genetic information into protein chains—is one of the oldest biological processes on planet Earth. Because of its age, all organisms, after the last universal common ancestor, use the same basic components—such as the transfer RNA (tRNA); aminoacyl-tRNA …
-
Mechanisms of pain processing: spinal protein translation in the rat
… of long-term plasticity specifically require protein synthesis and such mechanisms are widely believed to be cellular counterparts of long-term memory. Relevant to mammalian pain nociceptors is the realisation that messenger ribonucleic acid (mRNA) in dendrites, axons, axon terminals as well …
-
The study of Murine double minute-2 (Mdm2) in regulating neuronal activity-dependent protein translation
… that contribute to elevating local synaptic translation efficiency, it remains largely unknown how Gp1 mGluR-mediated signaling regulates general protein translation. We identified a novel function of Murine double minute 2 (Mdm2), an ubiquitin E3 ligase, which is involved in the Gp1 …
-
A multiplexed approach for quantitative profiling of the translatome using bioorthogonal non-canonical amino acids
… gap in systems biology is the quantification of protein synthesis rate at any given time (i.e the Translatome). At this time, measurements of protein synthesis rates are limited to methods that use mRNA abundance as a proxy; however, there are regulatory steps on the level of translation that can …
-
Neuronal FMRP: facilitating learning in a critical brain network for zebra finch song acquisition and regulating synaptic miRNAs spatio-temporally
… by absent expression of the normal fragile X protein FMRP, presenting with a constellation of features including intellectual disability, connective tissue abnormalities, and impaired vocalization (speech and language). FMRP is an RNA-binding protein, and animal models of FXS present with …
-
Luminescence-Based MicroRNA Detection Methods
… into the cell cytoplasm where they regulate protein translation either through direct cleavage of mRNA, or indirect inhibition through binding to mRNA and disrupting the protein translation machinery. Recently, miRNAs have gained much attention due to their implication in numerous diseases …
-
Role of Xenopus p21-activated kinase 2 (X-PAK2) in polar body formation during oocyte maturation
… no mRNA synthesis is detectable. Instead, active protein translation from maternal mRNAs and post-translation modification are required for oocyte maturation. However, these protein translational events are by and large not known. In this study, I have studied the potential role of such a …
-
Identifying a role for nonessential elF3 subunits eif-3.K and eif-3.L in the regulation of endoplasmic reticulum homeostasis and longevity in Caenorhabditis elegans
The eukaryotic initiation factor 3 (elF3) is a protein complex composed of 13 subunits in mammals, and is an essential scaffold of the molecular interactions required for the formation of the 43S preinitiation complex (PIC). While these 13 subunits are broadly conserved within the eukaryotic …
-
The role of eukaryotic initiation factor 4E (eIF4E) in osteosarcoma metastasis
… (new tool) used to detect and validate protein biomarkers across a variety of patients and an ex vivo pulmonary metastasis assay (new reagent) that allows real-time assessment of metastatic progression in a relevant microenvironment. Furthermore, cancer cells are believed to efficiently …
-
Nutritional Supplementation and Skeletal Muscle Health in Chronic Diseases
… participants. Leucine has been shown to activate protein translation initiation pathway mTORC1 in skeletal muscle. Further, mTORC1 is also known for regulating a variety of cellular pathways. Thus, the second objective of the thesis is to determine the effect and mechanisms of leucine …
-
Migration of Nucleocapsids in Vesicular Stomatitis Virus-Infected Cells is Dependent on Microtubules and Actin Filaments
… a variety of cytoskeletal motors and adapter proteins. Viruses hijack these pathways to distribute their proteins and genetic material. Vesicular stomatitis virus (VSV) is a prototype Rhabdovirus that has been used to study transport within the cell. We developed a new method of analysis of …
-
Regulation of Matrix Metallopeptidase-1 in Breast Cancer Metastasis
… are unchanged in the cell variants, however its protein levels are higher in the metastatic cells. There was no change in protein degradation rates, while protein synthesis rates of Fra-1 increased. These results suggest that protein translation of Fra-1 is differentially regulated in these …
-
Cartilage mechanobiology and transcriptional effects of combined mechanical compression and IGF-1 stimulation on bovine cartilage explants
… responses at the level of gene transcription, protein translation and post-translational modifications. Intracellular pathways including those involving integrin signaling, mitogen activated protein kinases (MAPKs), and release of intracellular calcium have been confirmed in several …
-
CRISPR-based functional genomics to study gene regulatory architecture and consequences of genetic variation
… the genetic template for RNA transcription and protein translation, and translated proteins are the functional units of the cell that carry out biological function. Despite coding genes acting as templates for RNA transcription and protein translation, the noncoding genome contains the vast …
-
The Endoplasmic Reticulum Udpase ENTPD5 Promotes Cancer Cell Growth and Survival in the PI3K/PTEN
… AKT and downstream PI3 Kinase signaling promotes protein translation, resulting in increased protein flux into ER; this will lead to decreased efficiency of protein folding and accumulation of unfolded proteins in the ER and finally lead to ER stress. How does cancer cell solve this problem of …
-
Proteomics Study of Pre-Harvest Sprouting in Wheat
… revealed that 190 differentially expressed proteins might be involved in various cellular functions, such as carbohydrate metabolism, nitrogen metabolism, stress response, redox regulation, ATP synthesis, and protein translation, during this untimely germination of the wheat embryo. …
-
Tuning of Neuronal Excitation by a Brain Specific Microrna MIR-128: From Targets to Behavior
… gene expression. As ubiquitous regulators of protein translation, microRNAs have a unique regulatory function that is only beginning to be fully described. In addition, their role in the control of gene expression in neurons, which underpins neuronal identity and information processing, has …
-
Knowledge discovery and modeling in genomic databases
… features of the functional sites including protein-translation start sites, mRNA splicing junction donor and acceptor sites in vertebrate genes. The HMM system based on the developed models is fully trained using an expectation maximization (EM) algorithm and the system performance is …
-
Epidermal growth factor-mediated activation of the unliganded glucocorticoid receptor in A549 cells
… via the TLR-2 receptor but not via the protein kinase C (PKC) pathway. The EGF response is shown to act via the nuclear factor kappa B (NF-κB) promoter element in a GR-dependent manner. Some mechanistic insights into the regulation are provided by demonstrating that EGF results in …
Page 1 of 6