Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"protein transduction domain"”.
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Engineering bacteriophage nanocarriers for targeted delivery of protein reagents to prostate cancer cells
Proteinaceous reagents, including antibodies and synthetic proteins, have become some of the most effective reagents for targeted treatment and diagnosis of disease. The unique catalytic activity of some proteins and ability to bind disease-relevant receptors that can evade small molecule …
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Development of Shell-Crosslinked Knedel-like Nanoparticles as Intracellular Delivery Vehicles and Gene Regulation Agents
… involved in this dissertation include protein transduction domain: PTD)-functionalized SCK, folate-functionalized SCK and cationic SCK: cSCK). Biological evaluation of each type of nanoparticles is described. For the PTD- and the folate-SCKs, a particle shape/size dependence on their …
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Mechanistic study of aryl hydrocarbon receptor nuclear translocator (ARNT)-mediated signaling
… interacts with the helix-loop-helix (HLH) subdomain of ARNT, but not with HIF-1α. GFP-Ainpl is localized in both cytoplasm and nucleus, and suppresses HIF-1α signaling by two mechanisms: (1) cytoplasmic GFP-Ainp 1 retains ARNT in the cell cytoplasm and (2) nuclear GFP-Ainpl inhibits …
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Design and synthesis of aryl hydrocarbon receptor fusion proteins for polyclonal antibodies production and cellular delivery
… mechanism, our lab has generated many human proteins using recombinant DNA technology. This thesis documents the design and synthesis of a number of proteins of the AHR deletion construct CΔ553. The bacterial expressed and purified fusion proteins could be utilized as antigen to …
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Intracellular delivery of rabbit monoclonal antibody
… the past decades, a series of small peptides, Protein Transduction Domain (PTD), were discovered to be able to facilitate the delivery of small proteins into living cells. With the specific feature, researchers have successfully delivered some functional proteins into living cells. To fully …
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Role of BCL-XL in cell death after spinal cord injury
… regulated in part, by SCI-induced decreases in protein levels of the anti-apoptotic protein Bcl-xL. In this dissertation, I assessed the role that Bcl-xL subcellular re-routing and post-translational phosphorylation play in SCI-induced Bcl-xL decreases, and evaluated the therapeutic potential of …
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Structural transitions in self-assembled lipid systems driven by induced curvature: from cell-penetrating peptides to programmable vesicles
… with model cell membranes, and find that the transduction activity correlates with induction of negative Gaussian curvature. The negative Gaussian membrane curvature is broadly enabling and its induction can lower the free energy barriers for a range of different entry mechanisms, such as …
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Charge and membrane curvature induced transitions in biomolecular systems: from actin bundles to cell-penetrating peptides to Bcl-2 cell death regulation in the mitochondria
Item reinstated by Sarah Shreeves (sshreeve@illinois.edu) on 2014-02-01T11:00:26Z Item was in collections: Graduate Theses and Dissertations at Illinois (ID: 204) Dissertations and Theses - Physics (ID: 445) No. of bitstreams: 2 Lai_GheeHwee.pdf: 9845710 bytes, checksum: …