Global ETD Search
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Showing 1 to 20 of 56 for “"protein trafficking"”.
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Live cell imaging of protein trafficking: Dynamics and functional insights
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2026-08-01
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Defects in protein trafficking affect expression of GPCRs in C. elegans
… the ventral nerve cord to the head. Several G protein coupled receptors (EGL-47, SRA-6) are terminally expressed in PVQ as well as a limited number of other neurons. Mutations in six genes (tba-1, unc-14, unc-33, unc-44, unc-51, unc-119) known to disrupt axon outgrowth were found to eliminate …
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Protein Trafficking of BK Channel β1 Subunits in Cerebral Artery Myocytes
… the hypothesis that ET-1 inhibits β1 surface trafficking in myocytes via activation of PKC. Test the hypothesis that activation of PKC directly modulates Rab11A through phosphorylation. Furthermore, test the hypothesis that trafficking of pore- forming BK channel (BKα) and auxiliary β1 …
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Molecular mechanisms of TOM1-mediated protein trafficking in health and disease
TOM1 is an adaptor protein involved in the alternative ESCRT-0 pathway. TOM1 functions by recognizing the ubiquitin moieties on internalized receptors and sorting them towards the degradative arm of the lysosomal system. Two mechanisms of TOM1 aberrant function were uncovered: one mechanism due to …
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Using high-content subcellular proteomics to identify x-ray-induced protein trafficking
… molecules are located within the cell. Many proteins have multiple roles and these functions can be dependent on their subcellular localisation. More traditional methods for measuring subcellular localisation of proteins, such as microscopy are lower throughput, are biased/targeted and can be …
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New reporters of protein trafficking and protein-protein interactions in live cells
… to harness the exquisite specificity of natural protein and RNA enzymes to develop improved methods to study protein localization and protein-protein interactions in live cells. We first attempted to detect endogenous protein-protein interactions (PPIs) in live cells by means of a ribozyme …
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The Function of Phosphatidylinositol 4-Kinase III-Beta in Trypanosoma Brucei
… transduction, endocytosis, exocytosis, and protein trafficking. Two phosphatidylinositol 4-kinases are found in Trypanosoma Brucei. We have cloned the gene encoding the Type III phosphatidylinositol 4-kinase Beta (TbPI4KIII-ß) in Trypanosoma Brucei. The protein was exogenously expressed in …
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Defining the regulatory determinants in substrate catalysis by biochemical, biophysical, and kinetic studies for the development of specific small-molecule inhibitors of ubiquitin specific proteases 7 and 17
… processes, including the cell-cycle, membrane protein trafficking, endocytosis and apoptosis. Ubiquitin Specific Proteases (USPs) remove ubiquitin modifications from protein substrates to reverse the signal imposed by the ubiquitination. Perturbations in the expression levels of USPs has been …
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Cornichon Proteins: Unexpected Roles in Plant Pathogen Infection, ER Morphology Maintenance and Pollen Development
Cornichon (CNI) proteins are a conserved family of proteins among eukaryotes, from Erv14 in the yeast Saccharomyces cerevisiae to CNI homologs (CNIHs) in mammals and plants. Erv14 functions as a cargo receptor of coat protein complex II (COPII) for protein trafficking from the endoplasmic reticulum …
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Light field microscopy for 3D imaging flow cytometry
… cellular heterogeneity when imaging protein trafficking in live cells. The development of an optical system suited to large depth-of-field, multi-channel 3D imaging of flowing samples is presented. Design considerations in the excitation optics and the use of a light sheet in …
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Investigations into the Nature of the Endosomal System in Plasmodium falciparum
… the parasite must transport newly synthesized proteins and endocytosed cargo to a variety of organelles, many of which are formed de novo and have no human equivalent. This process in mammalian cells would utilize an endosomal protein trafficking system, but no endosomal structures or proteins …
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Examination of auxin transport and root development in the scd1-1 mutant in Arabidopsis thaliana
… plant cells via asymmetrically localized carrier proteins. This polarity is believed to be established and maintained by vesicular delivery and endosomal cycling of these proteins. The SCD1 (stomatal cytokinesis defective) protein has a domain structure that suggests it may function in Rab …
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Synaptojanin1 is involved in endolysosomal trafficking in cone photoreceptors
… require the ability to properly sort and traffic proteins to their correct subcellular destination. The production of new proteins must be balanced by the turnover of old and damaged proteins. A breakdown in this process is detrimental to any cell; however highly polarized, non-proliferating …
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Characterisation of calcium/calmodulin-dependent protein kinase kinase 2 in prostate cancer
… of the COPI coatomer complex which traffics proteins from the Golgi to the ER. The Gemin4 interaction is mediated by the Novel CaMKK2 Binding Motif; a nine aa consensus sequence present within Gemin4 which mediates interactions of import to the CaMKK2 and Gemin4 interactome. ARCN1 is …
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Vps1's Role From the Early Endosome to the Golgi
Vps1 is a dynamin-like protein that has been implicated in various cellular events, including protein trafficking from the late Golgi-to-endosome, peroxisome fission, vacuole fission and fusion, and retention of Golgi proteins. We recently observed in FM4-64 pulse-chase experiment with vps1 null …
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The mechanism of HIV-1 Nef-mediated downregulation of CD4
Nef, an accessory protein of HIV-1, is a critical determinant of viral pathogenicity. The pathogenic effects of Nef are in large part dependent on its ability to decrease the amount of CD4 on the surface of infected cells. Early studies suggested that Nef induces downregulation by linking the …
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Systematic Identification of Interaction Partners of RET Receptor Isoforms
… 3’ end of the RET gene produces two conserved protein isoforms, RET9 and RET51, that differ in their subcellular localization and protein trafficking, and their functional roles in tumorigenesis and metastatic processes, suggesting that they may also differ in their interactomes. We used a …
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Novel Roles of PACS-1 Within the Nucleus and the Regulated Secretory Pathway
… sections: the investigation of PACS-1 nuclear trafficking, the impact of PACS-1 on calcium homeostasis, and PACS-1 regulation of membrane protein trafficking within the regulated secretory pathway. First, our results establish PACS-1 motifs that regulate nuclear localization through the …
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P125, A COPII INTERACTING PROTEIN
Coat protein complex II (COPII) coated vesicles/carriers mediate the export of proteins from the ER via specialized domains known as the ER exit sites (ERES). p125A is a protein of 125 kDa that interacts with Sec23A, a component of the COPII coat. It was found to participate in the organization of …
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The Tumor-Suppressive Role Of Secreted Maspin Via The Exosomes
… how maspin is regulated at the step of protein trafficking. The data showed that maspin is secreted by dual mechanisms, as free and exosomal protein, respectively. These two mechanisms seem to be independent. While tumor cells are capable of secreting maspin as a free molecule, albeit at …
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