Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"protein targeting"”.
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Expression of microbial rhodopsins in retinal neurons with subcellular targeting motifs: for the study of the structure/function of aii amacrine cells and for vision restoration
<p>Protein-targeting motifs serve as addresses for subcellular protein localization. This feature of targeting-motifs was used to study the retina. The first part of the dissertation reports in the axonless spiking AII amacrine cell of the mammalian retina a dendritic process sharing organizational …
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Age related memory loss and the role of the ubiquitin proteasome system
… challenges, such as the accumulation of damaged proteins. The ubiquitin-proteasome system (UPS), responsible for breaking down damaged proteins, exhibits dysfunction in neurodegenerative disorders like Alzheimer's disease or dementia. While we acknowledge that proteasome function declines with …
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The rise and fall of structure in physics : polaritonic photonic crystals, melting, and min-protein oscillations
… and stochastic models of the Min-protein oscillations in bacteria that reproduce all main experimental observations. These models explain the origin of instability that ultimately causes dynamic pattern formation and have successfully been used to predict nucleotide binding rates …
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The Role of the SCD1 Protein in Targeting PIN2 Protein in Arabidopsis Thaliana
… the asymmetrical localization of auxin transport proteins in the plasma membrane. The <italic>SCD1<italic> (stomata cytokinesis defective) gene encodes a protein containing a DENN domain, which is predicted to function as a RAB-GEF, which may be important in membrane protein targeting. The …
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Targeting CDK to Prevent and Treat Retinoblastoma and other Cancers
… drug sensitivity is predicted by a subset of Protein Processing networks (e.g. translation, mRNA stability, splicing, and protein targeting). Through mining publically available datasets, we unravel a novel co-regulatory/co-dependent relationship between CDK2 activity and Protein Processing …
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The Role of HSP90C in Chloroplast Maturation and Thylakoid Protein Transport
… in green plants and contains thousands of proteins. Since most chloroplast proteins are synthesized outside of the chloroplast on cytosolic ribosomes, they are imported into chloroplasts in mostly unfolded states. A protein quality control system in the chloroplast is then responsible for …
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Engineering Hv1 Channel Mutations and Targeted Drug for Treatment of Hv1 Mutation Associated with Cancer
… channels, such as differential expression and protein targeting issues. The second aim employs the patch-clamp technique to investigate the effects of mutations on Hv1 channel gating and its implications for disease progression. Finally, in the third aim, we focus on the development of targeted …
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Analysis of essential structural and functional elements in the GTP-regulated signal recognition particle cycle
… signal recognition particle (SRP)-mediated protein targeting using a combination of genetic, biochemical, and molecular sequence analysis techniques. First, I consider the SRP cycle from the perspective of molecular evolution. This analysis provides insight into the significance of …
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Dissecting the Role of the Lipodystrophy Protein Seipin in the Biogenesis of the Lipid Droplet Organelle
… forces, or a regulated process driven by protein factors. The Goodman laboratory previously found evidence to suggest that seipin, mutated in the most severe cases of congenital generalized lipodystrophy, may be a key factor in the early stages of lipid droplet formation. Seipin resides at …
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Using Chemically Modified Oligonucleotides to Modulate Gene Expression, Treat Genetic Diseases, and Probe Novel Mechanisms of RNA Interference
… (HTT) and production of toxic glutamine-expanded protein. Targeting HTT with siRNAs could be a powerful approach, but allele-selectivity is a major challenge: nearly all HD patients are heterozygous at the HTT locus, and expression of wild-type HTT may need to be preserved. One way to achieve …
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Biosensor design utilizing particle-bound enzymes
… This thesis explores whether recombinant protein technology in combination with a low-cost support matrix could provide a basis for an inexpensive, simple, and robust production process for the bio-sensing element of a diagnostic that would be amenable to manufacture in resource …
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Global analysis of mRNA decay rates and RNA-binding specificity reveals novel roles for CUGBP1 and PARN deadenylase in muscle cells
… cells expression and function of the RNA-binding proteins CUGBP1 and MBNL1 are disrupted, resulting in altered mRNA metabolism at the levels of splicing and translation. Intriguingly, despite strong evidence for CUGBP1 being a regulator of mRNA turnover in humans and other organisms, the …
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Regulation of Polarized Protein Transport to Axons, Dendrites, and Sensory Cilia in Caeborhabditis Elegans Neurons
… receptors, synaptic vesicles, and signaling proteins. Yet, despite the significance of asymmetric protein transport in neuronal polarity, the molecules and mechanisms that direct polarized transport to axons, dendrites and cilia in neurons are only partly understood. In this thesis, I …
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Vergleichende Analyse herpesviraler Amplicon-Vektoren am Beispiel von Antigenen des Humanen Papillomvirus-16
… anhand des Modellantigens L1, dem Hauptkapsidprotein des humanen Papillomvirus-16 (HPV-16), die zwei verschiedenen Verpackungssysteme bezüglich Verpackungseffizienz, Helfervirus-kontamination und Transgenexpression untersucht. HPV-16 wird als ein wichtiger Faktor bei der Entstehung des …
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Protein SUMOylation is a Sex-Specific Regulator of Fear Memory Formation in the Amygdala
SUMOylation is a type of post-translational protein modification similar to ubiquitination and it involves the covalent attachment of a small ubiquitin-like modifier (SUMO) protein to the lysine residue of a target substrate. While there is strong evidence for the role of protein ubiquitination in …
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Modifying N-linked glycosylation in tobacco to produce human-type post-translational modifications
… are a capable system for producing high value proteins for a variety of applications, and challenge popular host organisms such as mammalian cells, bacteria or yeasts. However, plants do not perform post translation protein modification in the same manner as mammalian cells, most critically in …
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Rationally designed aplyronine analogues for use in antibody-drug conjugates
… in human cancer cell lines. Through a novel dual protein-targeting mechanism of action, their exquisite potency renders the aplyronines promising drug candidates. However, the scarcity and structural complexity of the aplyronines brings challenges in supplying material to enable these studies, and …
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Multimethodological approaches to tackle glycogen: therapeutic and modelling opportunities in Lafora disease
Protein aggregation is a key pathological hallmark of many neurodegenerative diseases. However, such a paradigm does not universally prevail. Indeed, some neurological glycogen storage disorders manifest through the accumulation of aberrant glycogen into polyglucosan bodies (PGBs). These include …
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Targeting Ion Channels to Distal Dendrites
… achieved by localizing different complements of proteins to these compartments; axons contain a high density of voltage-gated channels, presynaptic boutons house the machinery for synaptic vesicle-mediated neurotransmitter release, while dendrites contain ligand-gated and voltage-gated ion …
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Unterschiedliche Funktionen von SRP und SecA beim Transport eines bakteriellen Proteins
… aus Innenmembran, Periplasma und Aussenmembran. Proteine dieser Kompartimente müssen nach ihrer Synthese im Zytosol an ihre Bestimmungsorte transportiert werden. Für die Zielsteuerung ungefalteter Proteine an die Innenmembran und ihren anschliessenden Transport sind im wesentlichen zwei …
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