Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 43 for “"protein sorting"”.
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Protein sorting to the apical membrane of epithelial cells
… research. Rafts have been proposed to act as sorting platforms during biosynthetic transport, especially along pathways that deliver proteins to the apical membrane of polarised cells. Based on this, the aim of this work was to contribute to the understanding of apical sorting in epithelial …
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Lipid rafts in protein sorting and yeast cell polarity
The major sorting station of biosynthetic material destined for the cell surface or secretion is the trans Golgi Network, TGN. This organelle sorts proteins and lipids into vesicular transport carriers that are targeted via different pathways to distinct membrane compartments of the cell. The …
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Lipid and Protein Sorting by, and Generation of, Membrane Curvature in Model Systems
… membrane enriched in specific lipids and proteins to enable complex processes – has led naturally to questions concerning the sorting of both lipids and proteins between membranes of different organelles within the cell, each with distinct lipid and protein composition. The shapes and, …
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Regulation of COPII vesicle formation and protein sorting by the BST genes in Saccharomyces cerevisiae
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1998.
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The role of the Dgk1 and Pah1 in lipid interconversion on saccharomyces cerevisiae vacuole physiology, homeostasis, protein sorting and fusion
… of the yeast vacuole, including a number of proteins and regulatory lipids. The deletion of the phosphatidic acid (PA) phosphatase gene Pah1, which converts PA in diacylglycerol kinase (DAG), was previously shown to produce a fragmented vacuole phenotype, greatly inhibit fusion and result in …
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Yeast Dynamin Functions with Clathrin at the Golgi
Vps1 (vacuole protein sorting) is a GTPase protein and a yeast ortholog to the mammalian dynamin-like protein (Dlp1). Vps1 acts cooperatively with many proteins at diverse cellular locations for endocytosis, protein sorting, and membrane fusion and fission. Classical dynamin family proteins work in …
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Regulation of Traffic into and out of the Yeast Endosome by the VPS9P Cue Domain and the VPS5P Domain
… and biochemical analyses of the yeast vacuolar protein sorting pathway have defined the steps in this process and identified more than 40 gene products involved. Soluble vacuolar hydrolases are diverted from the secretory pathway through interaction with a vacuolar protein sorting receptor in …
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Characterization of Class D VPS Proteins
… lysosome. The components of the VPS (vacuolar protein sorting) system are responsible for proper delivery of vacuolar biosythetic enzymes. Efforts to dissect the genetics of this system have revealed several classes of mutants, each defective in one transport step in the VPS pathway. The Class …
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Novel Physical Interactions of Vps1 with Major Intracellular Traffic Regulators
Vacuolar Protein Sorting 1 (Vps1), the yeast homolog to human dynamin, is a GTPase that acts as a pivotal component in membrane trafficking. In light of observations that VPS1 displayed positive genetic interactions with four genes implicated in the recycling traffic (YPT6, GYP1, RCY1, and RIC1) …
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With No Lysine 1 (WNK1): A Potential Regulator of The Lysosomal Degradation Pathway
… (K) 1 (WNK1) is an atypical serine/threonine protein kinase that has its catalytic lysine positioned in a unique location. This kinase, along with another member of the WNK family, WNK4, has been genetically linked to pseudohypoaldosteronism type II (PHAII), which is characterized by both …
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"Molecular Analysis of Virulence Factors Unique to Leptopilina heterotoma-a Parasitic Wasp of Drosophila spp."
… oviposition, the female wasp introduces venom proteins and virus-like particles (VLPs) into developing fly larvae. VLPs are endocytosed by plasmatocytes, a macrophage-like blood cell that is the most abundant hemocyte present in the circulating hemolymph. Once internalized, VLPs destroy …
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Lst1p and Exp1p act in parallel pathways to export the plasma membrane H⁺-ATPase from the ER in S. cerevisiae
Efficient transport of proteins to the correct intracellular compartment is critical for maintaining the functional integrity of the cell. Proteins destined for export from the ER are sorted from ER resident proteins and packaged into vesicles coated with the COPII protein complex. To facilitate …
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Luminal calcium regulates membrane fusion in the early secretory pathway
… has an inhibitory effect on homotypic coat protein complex (COPII) vesicle fusion. In particular, chelation of luminal calcium as well as fast chelation of extra luminal calcium, result in a significant increase of COPII vesicle fusion. Specific inhibition of Sarco/endoplasmic reticulum …
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Luminal calcium regulates membrane fusion in the early secretory pathway
… has an inhibitory effect on homotypic coat protein complex (COPII) vesicle fusion. In particular, chelation of luminal calcium as well as fast chelation of extra luminal calcium, result in a significant increase of COPII vesicle fusion. Specific inhibition of Sarco/endoplasmic reticulum …
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Development of a Biosensor for Investigating Membrane Curvature Sorting
… membranes plays a critical role in lipid and protein sorting. A novel biosensor was developed to probe the influence of curvature on sorting. This biosensor mimics large, two-dimensional membranes in dynamic equilibrium, achieves high spatial resolution between curvature and molecules of …
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Localization of Human Prostaglandin E2 Receptors in Polarized Epithelial Cells
The underlying mechanisms of protein sorting in polarized epithelial cells are poorly understood. Several studies have determined membrane targeting of G protein-coupled receptors (GPCRs) using epithelial cells such as Madin-Darby canine kidney (MDCK) cells. Polarized epithelial cells are composed …
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MOLECULAR RECOGNITION OF THYROGLOBULIN BY SORTILIN
Thyroglobulin (TG) is a large glycoprotein essential for thyroid hormone synthesis and storage. Synthesised and secreted by thyrocytes, TG forms a dimeric structure within the thyroid follicle lumen, where it undergoes iodination and hormonogenesis before being retrieved through endocytosis for …
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The effects of cis- and trans-acting mutations on recombinant protein secretion in Pichia pastoris
… research and industrial settings for recombinant protein expression due to the ease of genetically modifying its genome, its ability to grow to large densities in inexpensive media, and its capability to perform posttranslational modifications. Multiple tools such as the cis -acting factors MATα …
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FOOD SECURITY: INNOVATIVE PLANT REPRODUCTIVE STRATEGY IN A CHANGING ENVIRONMENTAL CONDITION
… a caratterizzare la funzione del gene VACUOLAR PROTEIN SORTING 13 (VPS13), presunto regolatore della formazione di gameti femminili non ridotti e clonali nello sviluppo del tarassaco, e dell'ovulo nella specie sessuale modello Arabidopsis thaliana, valutandone i meccanismi a valle. A causa della …
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PI(4)P-Dependent Recruitment of Clathrin Adaptors to the Trans-Golgi Network
… Trans Golgi Network (TGN) is the cell's central sorting station, and the complex trafficking patterns are organized by many types of trafficking adaptors. These include the heterotetrameric adaptor protein complexes (APs) and the monomeric Golgi-localized, gamma-ear containing, Arf-binding …
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