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Showing 1 to 6 of 6 for “"protein semi-synthesis"”.
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Protein Semi-Synthesis in Vivo
<p>Incorporation of chemical probes into proteins is a powerful way to elucidate biological processes and to engineer novel function. This thesis describes the ligation of synthetic molecules to target proteins in an intracellular environment, and the use of semi-synthetic proteins for whole animal …
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Split Inteins: From Mechanistic Studies to Novel Protein Engineering Technologies
<p>Inteins are auto-processing protein domains that carry out a post-translational process known as protein splicing. This process is characterized by excision of the intein (intervening protein) domain from within a larger polypeptide sequence with concomitant ligation of the flanking extein ( …
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Semi-Synthesis of the Transcription Factor SMAD2 Containing Caging Groups and Phosphoaminoacid Analogues
<p>Post-translational modification (PTM) of a protein refers to any chemical change that occurs to the protein after its ribosomal synthesis. The seemingly endless number of PTMs can endow proteins with new functionalities that are not present in the unmodified proteins. In order to study the …
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Site-Specific Incorporation of Biochemical and Biophysical Probes into Proteins Using Expressed Protein Ligation
<p>Protein engineering can be made far more powerful if a protein is not only expressed recombinantly but also altered covalently using synthetic chemistry. These two methods are brought together in the protein semi-synthesis technique Expressed Protein Ligation (EPL). In EPL, recombinant and …
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Multifunctionalization of Proteins: Strategies for Combining Semi-Synthetic and Bioorthogonal Techniques
Proteins play important roles in biological processes including muscle movement, initiating immune responses, and memory formation. Full understanding of protein function requires determining its structure, assignment of function, and elucidation of interactions with other proteins or metabolites. …
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Chemical Modification Methods for Protein Misfolding Studies
Protein misfolding is the basis of various human diseases, including Parkinson’s disease, Alzheimer’s disease and Type 2 diabetes. When a protein misfolds, it adopts the wrong three dimensional structures that are dysfunctional and sometime pathological. Little structural details are known about …