Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"protein scaffolds"”.
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Metalloprotein Engineering Using Heme Protein Scaffolds to Investigate the Oxidation of Endogenous Aromatic Amino Acids and Exogenous Substrates
Protein engineering by rational design has been used to study heme proteins and artificial Mn-salen containing metalloenzymes using heme protein scaffolds. Heme proteins perform a wide variety of functions including the catalysis of numerous different reactions. Some of the structural features …
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Endogenous and chemical modifications of model proteins
Protein modifications are ubiquitous in nature, introducing biological complexity and functional diversity. Of the known post-translational modifications, glycosylation is one of the most common and most complex, yet some of the biological implications of this modification remain poorly understood. …
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Encapsulation of Achiral Mn(Salen) Complexes Into Cytochrome C Peroxidase: Spectroscopic Characterizations and Epoxidation Studies
… can also be incorporated into CcP or other protein scaffolds to produce a library of biocatalysts to perform various organic transformations.
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Understanding the Speciation of Ruthenium Arene Complexes as Precursors to Unnatural Enzyme Cofactors
… many potential Lewis basic ligands provided by protein scaffolds. Combining nuclear magnetic resonance (NMR) spectroscopy, mass spectrometry (MS), X-ray crystallography and other biophysical characterisation techniques, the speciation of ruthenium arene bipyridine complexes with small molecule …
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Biological and biomimetic mineralization via protein nanoribbon scaffolds
Understanding how protein scaffolds direct mineral morphogenesis is crucial for engineering bone and tooth and would open new vistas in hybrid materials design. In the case of tooth enamel, which is the hardest tissue in the body and consists of organized bundles of coaligned filamentous apatite …
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Approaches towards development of novel fluorogenic biosensors for detection of small protein analytes
… must be developed to quantitatively track native protein analytes in live cells. Herein approaches toward the development of a set novel fluorogenic biosensors capable of detecting native ErbB-ligands are reported. Using yeast surface display, two libraries of protein-binding scaffolds were …
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Harnessing the Anaphase-Promoting Complex for Targeted Protein Degradation
Targeted protein degradation (TPD) is rapidly becoming a prevalent modality of therapeutics development due to its event-driven pharmacology that overcomes some major limitations of conventional small molecule inhibitors. One of the paradigms of TPD involves co-opting components of the …
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Exploring Structure Function Relationship using Bio-Inspired DNA-Chromophore Complexes
… with nanoscale precision using a network of protein scaffolds. However, novel protein molecules are difficult to systematically manipulate. We adopt a DNA-based framework to scaffold cyanine chromophores in order to construct modularizable photonic circuit components. This framework allows …
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Computational Investigation of the Catalytic and Structural Roles of Metals in Metalloenzymes
… on the unique roles of metal co-factors and protein scaffolds in catalyzing crucial chemical transformations at ambient conditions with exquisite selectivity. Some metalloenzymes exploit the redox properties of metal cofactors to catalyze challenging reactions, while others recruit metals for …
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Recruitment of Enzyme Cascade to Phase-Separated Biomolecular Condensates Accelerates Reactions via Concentration-Dependent and Concentration-Independent Mechanisms
… in vitro model system consisting of multivalent protein scaffolds and a minimal enzyme system-the SUMOylation cascade. By inducibly recruiting various combinations of components of the SUMOylation cascade to condensates, we are able to uncouple the contributions of individual components and …
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Engineering Biocatalytic And Living Materials
… the self-assembly and biosilification of fusion proteins. The scaffold forming protein EutM was genetically fused to four different silica biomineralization peptides, enabling control over the scaffold morphology and extent of biosilicification. At high silica concentrations, the proteins …
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Uncovering Biophysical Determinants Of Oxidoreductase Function Through De Novo Protein Design
… and cellular respiratory machinery, “redox proteins” and the catalysis they enable form the foundation biological energy transduction. Mastering the mechanisms by which these enzymes work is the key to understanding how life works and drives the improvement of an as of yet poorly developed …
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Engineering developable miniprotein ligand scaffolds
… therapeutics and diagnostics, yet discovery of protein ligands is hindered by the vast, sparse, and rugged sequence-function fitness landscape. Protein scaffolds, characterized by a conserved framework to support an engineered variable active site, have proven effective in navigating this …
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Protein Engineering for Biomedicine and Beyond
… specificity and selectivity for their target. Protein engineering enables the design of scaffolds that can bind targets of interest while increasing their stability, and expanding the scope of applications in which these scaffolds will be useful. Repeat proteins are instrumental in a wide …
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Protein Engineering for Biomedical Materials
The inherent design freedom of protein engineering and recombinant protein production enables specific tailoring of protein structure, function, and properties. Two areas of research where protein engineering has allowed for many advances in biomedical materials include the design of novel protein …
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Bio-inspired iron and cobalt complexes featuring a secondary coordination sphere: ligand design, complexation, and modeling of enzymatic processes
… and cellular signaling. Metallocofactors and protein scaffolds often work in conjunction to attract and bind the small molecules, stabilize reactive intermediates, and shuttle protons and electrons to and from the active site. However, due to the size and dynamic nature of the protein scaffold …