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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"protein refolding"”.
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A novel in vivo protein refolding technique
<p>Proteins perform their functions in their native folded states and misfolding of proteins may cause severe diseases, including Alzheimer's disease, Parkinson's disease, prion disease and diabetes. Understanding protein folding is important for us to engineer proteins to treat these diseases. For …
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Protein refolding via immobilisation on crystal surfaces
… it possible to find an easy, generic method for protein refolding? The preparation of functionally active protein molecules from the unfolded state can be a difficult task. Although there are many well-established techniques for protein refolding, such as dilution, dialysis, chromatography and …
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Early Events in the Folding of the Protein Ubiquitin
… of several mutants of the small single-domain protein ubiquitin have been characterized, with an eye towards describing the early folding events. These events have been interrogated by a fast laser temperature-jump technique and by low temperature stopped flow mixing. In the case of the T-Jump, …
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Redox Sensing By Yeast Hsp70 Facilitates Modulation of Protein Quality Control and The Cytoprotective Response
… of these aggregates is damage sustained to proteins by oxidative stress. Cellular protein homeostasis (proteostasis) relies on the ubiquitous Hsp70 chaperone family. Hsp70 activity has been previously shown to be modulated by modification of two key cysteines in the ATPase domain by …
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Soluble and Functional Overexpression of the Ligand Binding Domain of Mouse Aryl Hydrocarbon Receptor in <i>E.Coli</i>
… solubility enhancing tag called maltose binding protein (MBP) was used for purification of mAhR aa211-384 under native conditions, which still did not yield soluble overexpression. The strategy was modified to solubilize the protein by denaturation with the use of 8M Urea, which solubilized the …
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Exploration of novel activin A antibodies
… complex with its pro-domain (pro-activin A). A protein refolding strategy was utilised to produce the antibodies as recombinant fragment antibodies (Fabs) in E. coli with high purity and homogeneity for downstream characterisation. B52 was discovered to bind activin A at its type II receptor …
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Consistent Fabrication of Ultrasmall PLGA Nanoparticles and their Potential Biomedical Applications
… and gene delivery, biodetection of pathogens or proteins, and tissue engineering. The aim of this study was to fabricate ultrasmall poly-(lactic-co-glycolic acid) nanoparticles (PLGA NPs) using a quick and easy nanoprecipitation method1, with some modifications, for general use in various …
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Energy Stress Causes Chaperones to Assemble Into Cytoplasmic Complexes
<p>The majority of proteins require molecular chaperones to assist their folding into tertiary and quaternary structures. Certain stresses can compromise the weak hydrophobic forces responsible for these structures and lead to protein unfolding, misfolding, and aggregation. Aggregates of proteins …
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A Proteomics Approach to the Examination of Proteins in Marine Systems
… source material contributions and microbial protein production to contribute to a more thorough understanding of the marine carbon and nitrogen cycles. The examination of molecular organic biomarkers throughout an Arctic sediment core showed the dominant input in the area was from marine …
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Cofactors in Coronavirus Entry
… viruses accomplish this task by viral glycoprotein-mediated binding to host cells and fusion of virus and host cell membranes. For the coronaviruses, viral spike (S) proteins execute these cell entry functions. In my dissertation research I focused on understanding the coronavirus spike …
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Protein mis-folding and human disease
… chaperone, is a homopentameric<br/>plasma protein of 25kDa subunits. It binds to the amyloid fibrils of<br/>misfolded proteins, which cause amyloidosis in humans. SAP not only stabilizes<br/>amyloid fibrils but also protects them from proteolytic and cell mediated<br/>degradation. SAP has …
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Deciphering Functional Significance of Substrate-Binding Domain of Ssa1 on Heat-Shock Response and Prion Propagation
The Hsp70 (70kDa heat shock protein) is highly conserved in all species and has been implicated in a variety of important cellular functions such as heat shock response, prion propagation, protein folding and refolding, translocation across membranes and assembly of macromolecular complexes. A …
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Development of high-throughput screening assays to identify small molecule inhibitors of Plasmodium falciparum Hsp70-1
… cost-effective screening assays. Heat shock proteins (Hsps) are a special class of molecular chaperones essential for parasite survival and development within the human host. Hsps are also implicated in drug resistance. Hsp70 forms an integral part of the molecular chaperone system, …
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COMPOSTI AD AZIONE ANTI-AGGREGANTE ESERCITANO UN EFFETTO BENEFICO SU MODELLI CELLULARI DI ATROFIA MUSCOLARE SPINALE BULBARE
… Receptor (AR) gene. This mutation leads the protein to form intranuclear aggregates in the spinal cord and brainstem and cause the death of those specific cell populations. AR belongs to the nuclear hormone receptors superfamily and it works as a transcriptional factor after binding its …
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Investigation of the Gracilaria gracilis proteome response to nitrogen limitation
… and recovery. Putative identities of 22 proteins that were changed significantly (P<O.05) in their abundance in response to nitrogen limitation and recovery were obtained. The identified proteins functioned in a range of biological processes including glycolysis, photosynthesis, A TP …
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Characterization of Ebola virus VP35-dsRNA binding for drug development against Ebola virus disease.
The Ebola virus (EBOV) VP35 protein plays an important role in the inhibition of the initial innate immune responses to EBOV infection leading to Ebola virus disease development. In fact, VP35 interaction with the RIG-I like receptors (RLR) cascade components inhibits the interferon (IFN) …