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Showing 1 to 13 of 13 for “"protein recognition"”.
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Evaluation of the Protein Recognition Properties of Peptide Nucleic Acids
<p>The objective is to evaluate the ability of aminoacyl-tRNA synthetases (aaRS) to recognize the non-standard nucleic acid, PNA (peptide nucleic acid). PNA has immense potential in biomedical applications due to its increased thermostability and nuclease resistance over natural nucleic acids. PNA …
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CH-[pi] interactions play a central role in protein recognition of carbohydrates/
Carbohydrate-protein interactions play a central role in biology, but knowledge of the forces underlying them is limited. Carbohydrates are generally hydrophilic and therefore present unique challenges in their recognition. One underappreciated force involved in carbohydrate-protein interactions is …
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Structural Basis for Protein Recognition, Acyl-substrate Delivery, and Product Release by ACP in the Biosynthesis of Lipid A
<p>Acyl-carrier-protein (ACP) is the principal transporter of fatty acids, coordinating acyl-transfer among a vast network of diverse enzymes and biochemical processes. ACP association with protein partners is thought to be exceedingly transient. This paradigm has posed challenges for understanding …
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Total gene synthesis, bacterial expression and functional characterization of a recombinant human hemoglobin: Progress towards a blood substitute
… interactions, ligand discrimination and protein-protein recognition, a genetic handle is required. The use of total gene synthesis alleviates some of the laborious procedures associated with conventional cDNA cloning and circumvents the problems associated with the expression of human …
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Frustration of protein folding from in vitro to in vivo
Protein folding, a ubiquitous and vital biological process, where protein random coil transforms into certain conformation in order to fulfill its function. Misfolded protein which fails to acquire proper shape, not only loses its function, but can also cause fatal diseases. In this dissertation, I …
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Interactive Analysis of Protein Structure and Dynamics
… the structure-function relationship of proteins such as G protein-coupled receptors (GPCRs) requires detailed information on their three-dimensional (3D) structure and dynamics. Recent progress in the structural elucidation of receptor-G protein or -arrestin complexes yields valuable …
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Experimental and computational investigations of the stability and dynamics of cytochrome bb5s
… cytochrome b$\sb5.$ The goal was to investigate protein dynamics and how they may relate to stability and biological function.Computational studies used molecular dynamics simulations to model the solution structure of cytochrome b$\sb5.$ Simulations revealed conformational changes on the protein …
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Advances in Microfluidics and DNA Nanotechnology for Biomolecular Analysis
… and an understanding of biological systems. Proteins play a leading role in biology, by performing the majority of biochemical and biophysical processes that maintain homeostasis. As such, they and their interactions are probed as biomarkers and molecular machineries key to the analysis of …
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High-resolution NMR spectroscopic analysis of anticancer drugs, DNA and their interactions: 1. Platinum anticancer compounds - DNA interactions. 2. Anthracycline drugs - DNA interactions and modified DNA
… distortions may be relevant in understanding the protein recognition of the cisplatin-induced lesions. A new anticancer bisplatinum compound 1,1/t,t exhibits excellent cytotoxicity towards cisplatin-resistant cancer cells. The structure of the interstrand adduct of 1,1/t,t with a palindromic DNA …
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Super-aptamer bio-imprinted hydrogels : an investigation into the optimization and characterization of cross-linked polymeric materials displaying macromolecular amplified responses
… hydrogel with specific response to target proteins demonstrates an additional category of macromolecular signal amplification. This super-aptamer assembly provides the first example of using protein-specific aptamers to create volume changing hydrogels with amplified response to the target …
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Characterization of splicing mechanisms by single-molecule fluorescence
… vivo</i>, this process is mainly assisted by protein cofactors. However, the exact mechanism of protein-mediated splicing of group II intron RNA is still not known. </p> <p> In order to elucidate the mechanism of protein-mediated splicing of group II introns, we have studied the folding …
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Programming Molecular Devices using Nucleic Acid Hairpins
… serve as nucleation</p><p>sites for RNA folding, protein recognition signals, mRNA localization and regulation of mRNA degradation. On the other hand, </p><p>DNA hairpins in biological contexts have been studied with respect to forming cruciform structures that can regulate gene …
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Study Of Covalent And Non-Covalent Interactions In Ternary Systems Involving: Metal/DNA-RNA/Protein, Where Metal = Platinum(II), Palladium(II)
Ternary systems comprising DNA/RNA, proteins and one (or more) metal ion are generating increased interest due to its biological relevance. The knowledge gained from the study of these systems could provide important clues regarding the precise mechanism for transcription factors, repair proteins …