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Showing 1 to 19 of 19 for “"protein quantification"”.
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Microfluidic particle tracking technique towards white blood cell subtype counting and serum protein quantification
… upon to count white blood cell subtypes or serum protein from a drop of blood. Traditionally, researchers have counted white blood cell subtypes by capturing them. However, an elegant and more accurate way to do the same is by exploiting the transitory interactions between the antigen on the …
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Over-expression in Escherichia coli of the ToxA51 llama single domain antibody targeting Clostridium difficile enterotoxin A
… small-scale expression of this recombinant protein. Large-scale expression was achieved in bioreactor at 503 mg/L of recombinant protein. Protein quantification was performed using SDS-PAGE densitometry while binding activity against TcdA was assessed via Biacore. Our studies also led to …
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Outlining different perspectives for accurate MS-based proteomics in processed matrices - a case study on European feed control
… legislation has restricted animal-derived protein in feed. However, current official control methods only partially ensure compliance. Mass spectrometry (MS) offers a promising approach to ensure food and feed safety, though thermal processing may compromise its sensitivity. Hence, this …
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Digital Protein Analysis: Technologies for Protein Diagnostics and Proteomics through Single-Molecule Detection
Protein quantification tools are valuable in biological and biomedical research and in clinical applications because they enable measurements that elucidate physiological states, including disease states. Protein biomarker detection is likely to play a major role in patient health and aid in the …
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Image analysis of prostate cancer tissue biomarkers
… gene fusion status) and TATI (encoded by SPINK1) proteins in a large TMA of localized prostate cancer patients. We observed a mutually exclusive expression pattern, further supporting the idea of tailored treatment for genotypically different cancers. In the second and third studies we introduce …
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Quantification and functional characterization of Sinorhizobium meliloti chemotaxis proteins
… It is facilitated by Methyl-accepting Chemotaxis Proteins (MCPs) as part of a two-component signal transduction system. These receptor proteins are able to bind ligands and influence the state of the signal transduction system, ultimately controlling flagellar behavior. The chemotaxis system of …
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Precision Medicine in Immunotherapy and Lung Cancer
… findings. The first is a flow cytometry protein quantification experiment to investigate whether differential protein-coding gene expression led to similar differences in protein expression. The second study explores specific single nucleotide polymorphisms associated with the key …
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Method development and proteomic profiling of boar seminal plasma using mass spectrometry-based workflows
… followed by semi-automated on-bead protein capture, clean-up, digestion and LC-MS/MS analysis. The gel-based method data were acquired through data-dependant acquisition and protein identifications were generated through a database search (ParagonTM Algorithm of the ProteinPilotTM …
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Mechanics and Modeling of Postnatal Arterial Development in Mouse Models of Varying Elastin
… composition of extracellular matrix: ECM) proteins to produce a vessel with the right mechanical properties. Closed circulatory systems driven by a beating heart have pulsatile flow, requiring the large elastic arteries to have the ability to distend and recoil to dampen the pulsatile …
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An Insight into Alpha-Synulcien's Biological Function and Its Pathogenesis in Neurodegenerative Disease
… significant increase in amyloid beta -peptides. Protein quantification of apolipoprotein E (ApoE) a protein that has been associated with the development of Alzheimer's disease, demonstrated a 5-15 fold increase in symptomatic transgenic mice. Ablation of ApoE in alpha -synuclein transgenic mice …
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Benchmarking methods for label-free quantitative proteomics data analysis
Proteins are fundamental functional units in cellular processes. Inter-individual variation in the composition of proteins in cells — including differences in amino acid sequence and in abundance — mediate many of the effects of genetic variants on the risks and presentations of diseases. …
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Subcellular Fractionation Methods for the Quantification of Endogenous Protein in Human Liver Using Targeted Proteomics
… Recently, interest into human endogenous protein quantification using targeted quantitative proteomics has increased. These quantification measurements are useful in many aspects of drug discovery and development, including protein biomarker monitoring, the development of a more accurate …
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Conjugation of Anti-HER2 Monoclonal Antibody onto a PLGA-PEG Nanoparticle Using CuAAC Click Chemistry
… conjugates and the recovered protein is measured via Bradford assay, a colorimetric protein quantification assay method. Particle concentration is determined by a modified coulter counter made by Izon® called the qNano. This collection of data allows for determination of the …
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Estimation of Variation For High-throughput Molecular Biological Experiments With Small Sample Size
<p><strong>Motivation:</strong> In the quantification of molecular components, a large variation can affect and even potentially mislead the biological conclusions. Meanwhile, the high-throughput experiments often involve a small number of samples due to the limitation of cost and time. In such …
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Assembly, characterization and evaluation of a 3rd generation nanoparticle based drug carrier for metastatic breast cancer treatment
… breast cancer cells. Firstly, the conjugation of protein to the surface of liposome and PEGylated liposomes were investigated. Proteins with or without membrane binding domain were conjugated to liposome and PEGylated liposomes through covalent and non-covalent binding for comparison. A modified …
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Production and characterization of transgenic maize plants designed to improve iron nutrition
… successful integration into the maize genome. Protein analysis results further confirmed the expression of the transgenes in the maize seed endosperms. The highest phytase enzyme activity obtained from maize seeds was 5.527 units of enzyme per gram of seed (U/g). This was significantly higher …
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Creation and characterization of mice with a mutation disrupting binding of a transcriptional repressor of insulin-like growth factor 2
… their WT counterparts (P≤0.01). In contrast, protein quantification of IGF2 in serum of 77d mice did not differ between genotypes (P=0.16). We had hypothesized that mice with mutations disrupting the ZBED6 binding site in IGF2 would have increased muscle mass and reduced fat deposition at the …
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Investigation of the effect of ocean acidification on the haemocyte proteome of the South African abalone, Haliotis midae
… using isobaric tagging for relative and absolute quantification (iTRAQ) coupled with LC-MS/MS to investigate the proteomic response of H. midae haemocytes to reduced pH conditions representative of future predictions of ocean acidification. Four independent iTRAQ experiments were conducted where …
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In vitro modelling of cellular haemozoin and inhibition by β-haematin inhibitors and their derivatives
… parasites, containing 1337 previously identified proteins with an average abundance-weighted molecular weight of 40,483 ± 77 g/mol. With this, the protein mass per cell for red blood cells, chloroquine-sensitive and - resistant parasites were consistent across three protein quantification methods …