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Showing 1 to 20 of 181 for “"protein production"”.
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Protein production and applications of NMR in pharmaceutical sciences
The objective of Part I (Protein production for structural elucidation using NMR) is to design and develop strategies of producing amyloid beta protein for structural analysis using NMR. Amyloid beta oligomers are considered to be key elements causing Alzheimer’s disease and the elucidation of the …
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Physiological aspects underpinning recombinant protein production in Escherichia coli
Many biopharmaceutical projects require the production of recombinant protein in a bacterial host. Conventional procedures used for recombinant protein production (RPP) involve the rapid synthesis of the target protein. This results in the accumulation of unfolded protein, the induction of the heat …
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Characterizing the landscape of aminoacyl-tRNA synthetase protein production in Bacillus subtilis
… and the magnitude of their expression into proteins. While the biochemical function of many proteins has been uncovered, for most it is unclear how important native protein abundances are for cell fitness. Furthermore, linking changes in abundances with downstream effects on enzymatic …
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A directed evolution approach to engineering recombinant protein production in S. cerevisiae
The continued success of protein therapeutics has put a strain on industry's ability to meet the large demand. Creating a more productive expression host for the manufacture of these proteins is a potential solution. Although heterologous proteins are frequently made in organisms as disparate as E. …
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Genomic Analysis And Engineering Of Chinese Hamster Ovary Cells For Improved Therapeutic Protein Production
Protein biologics have transformed the field of medicine in recent years. These complex molecules are produced in living cells, primarily Chinese Hamster Ovary (CHO) cells. Due to the importance of these therapeutic proteins to disease treatment, it is essential to improve the efficiency of their …
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Recombinant Protein Production in Bacillus Species, Isolation and Methionine Production in Methionine-producing Microorganisms
… in developing commercial processes for the production of single isomers of CLA by biotransformation of LA using microbial cells and enzymes. However, the evaluation of these strains suggest that growth and LA isomerase production levels by these anaerobes are insufficient to support economic …
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Anaerobic Digester Liquor as a Source of Ammonium and Phosphate For Single-Cell Protein Production
… The conversion of sugar and ammonia into protein was measured with C. utilis and K. fragilis. The efficiencies of this conversion were about 21% and 24% respectively. A two-membered culture of C. utilis and Streptococcus sp. were tested for maximum growth with C. utilis and S. faecalis …
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Active hypothermic growth : a novel means for increasing total recombinant protein production by CHO cells
Recombinant human glycoproteins produced by Chinese Hamster Ovary (CHO) cells are an important class of therapeutic molecules and investigating means of improving the production rate and product quality of these glycoproteins is therefore of great interest. Culturing CHO cells under mild …
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Estimation of yield and maintenance parameters associated with single cell protein production on C-1 compounds
Call number: LD2668 .T4 1984 L43
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Estimation of yield and maintenance parameters associated with single cell protein production on C-1 compounds
Call number: LD2668 .T4 1984 L43
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Application of metabolic and biochemical engineering techniques for the enhancement of recombinant protein production in Escherichia coli
… excretion, as a means to enhance recombinant protein productivities. The recombinant model system is based on the formation of plasmid encoded cadA-LacZ fusion protein with $\beta$-Galactosidase activity under the control of the pH-regulated promoter. This recently developed expression system …
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Design of a cultivation medium for protein production in Pichia pastoris based on genome-wide biological understanding
… to grow to high cell densities can enable fast production cycles, agile process development, and potentially low production costs. While P. pastoris has already been engineered to produce antibodies with human-like glycoforms, titers are still lower than those typically achieved with CHO cells. …
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Lactogenesis Induction in Transgenic Virgin Pigs as a Model for Identifying Transgene Expression and Recombinant Protein Production
The porcine mammary gland can be used for the production of recombinant proteins by directing a transgene to the mammary gland with a milk protein gene promoter. In order to determine whether or not the protein will be expressed, the animals must be maintained at least through their first …
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Influence of Cutting Frequency and Starter Fertilizer on the Yield and Protein Production of Alfalfa Medicago Sativa L
Made available in DSpace on 2014-12-12T00:17:37Z (GMT). No. of bitstreams: 1 7105088.pdf: 3402428 bytes, checksum: 2cf7a349bc598dbb863c06bc6b28baf4 (MD5) Previous issue date: 1970
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Mammalian Cell Line Development Platform for Recombinant Protein Production: Expanding the Protein Expression Toolbox for Research and Drug Discovery Applications
<p>Recombinant proteins have revolutionized the biomedical industry, providing therapeutics for life-threatening diseases and protein reagents for research applications. BioMarin Pharmaceutical Inc. develops recombinant protein therapeutics to treat rare diseases including lysosomal storage …
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Nitrate Reductase Activity in Wheat (Triticum Aestivum L.) and Its Relationship to Grain Protein Production as Affected by Genotype and Spring Application of Calcium-Nitrate
Made available in DSpace on 2014-12-10T17:12:41Z (GMT). No. of bitstreams: 1 6910691.pdf: 5166704 bytes, checksum: a2f16548d1a1e86f1c28a195f791ddb2 (MD5) Previous issue date: 1968
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Gene Amplification for the Improved Secretion of Foreign Proteins in the Yeast Saccharomyces Cerevisiae
… plays a central role in maximizing yield for protein production. Generally, more copies of a gene generally lead to an increase in protein production. The 2$\mu$ plasmid of Saccharomyces cerevisiae has been used extensively as a vector for foreign protein expression because of its natural …
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The potential for plant-based Meat in Africa - a proposed new approach using a system design methodology
… the potential application of new plant-based protein technologies to Sub-Saharan Africa. It demonstrates the use of a system design methodology to evaluate, assess and select a new approach to protein production. This is an important topic, because global protein production systems are under …
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