Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"protein phosphatase 1."”.
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Protein Phosphatase 1 at the Kinetochore Regulates Chromosome Segregation
… and kinetochore kinases, including Aurora B. Protein Phosphatase 1 (PP1) plays a counteracting role to Aurora B to stabilize kinetochore-microtubule attachments and silence the SAC. The regulation of PP1 to modulate these functions, however, remains enigmatic. Using the biochemical tools …
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The regulation of chromosome segregation by Aurora kinase, protein phosphatase 1 and nucleolar protein UTp7
… during anaphase is regulated by the Cdc14 protein phosphatase, which is kept inactive in the nucleolus until anaphase onset. I show here that the predominantly nucleolar ribosome biogenesis protein Utp7 is also present at kinetochores and is required for normal organization of kinetochore …
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An Approach to the Total Synthesis of the Protein Phosphatase 1 and 2A Inhibitor Microcystin -Lr
The present study is an investigation of different synthetic routes to the natural product utilizing several protecting group strategies. Extensive studies were required to gain insight into the susceptibility of the intermediates to reaction conditions. This work has led to the first synthetic …
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Alternative splicing of the zebrafish myosin phosphatase targeting subunit, MYPT1, produces a novel isoform
<p>Alternative splicing of the zebrafish Myosin Phosphatase Targeting Subunit, MYPT1, produces a novel isoform (TV202). TV202 and the truncated TV202Δ ere shown to form an active complex with Protein Phosphatase 1 β (PP1β) via stress fiber assay. TV202 was also shown to be localized in the …
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Analysis of cell cycle surveillance mechanisms in meiosis
… The extent of the delay is modulated by protein phosphatase 1 (PP1), whose activity allows cells to overcome the checkpoint dependent delay in a process called adaptation. In this work, experiments in the budding yeast Saccharomyces cerevisiae are described that show that premature …
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Identification and characterization of signal components in early ABA signaling in Arabidopsis
… receptors PYRABACTIN RESISTANCE 1 and PYR1-Like protein (PYR1/PYLs), type 2C protein phosphatases (PP2Cs) and SNF1- related protein Kinase 2s (SnRK2s). In the presence of ABA, PYR1/PYLs bind to and inhibit PP2Cs, leading to the release of SnRK2s from suppression, to activate downstream events …
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Identifikation und Charakterisierung neuer Leptin regulierter Gene in der Beta-Zelle des endokrinen Pankreas
… Darüber hinaus hemmte Leptin die mRNA- und Proteinexpression von Secretogranin II (Scg II) in INS-1 Zellen. Scg II wurde mit immunzytochemischen Färbungen in den Vesikeln von INS-1 Zellen nachgewiesen. Leptin hemmte des Weiteren die mRNA- und Proteinexpression sowie die Enzymaktivität der …
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Identification of the RNA Cis-Elements that Interact with SRp30a to Regulate the Alternative Splicing of Caspase 9 Pre-mRNA
… of caspase 9 and the phospho-status of SR proteins, a conserved family of splicing factors, are regulated by chemotherapy and de novo ceramide via the action of protein phosphatase-1 (PP1). Two RNA splice variants are derived from the caspase 9 gene, pro-apoptotic caspase 9a and …
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Regulation of the unfolded protein response by GADD34 and CReP
<p>The regulation of protein synthesis and protein folding is crucial for normal cell function. The endoplasmic reticulum (ER) has crucial roles in safeguarding the correct folding and assembling of proteins through the use of ER molecular chaperones. Homeostasis disruption of the ER leads to …
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Stochastic modelling for control and interconnections of biological systems
… contradictory findings surrounding the CaMKII/protein phosphatase-1 pathway, showing that bistability can be sensitive to the absolute quantity of reactants present. The second part of this dissertation develops a novel input/output framework for ap- proximating the stationary relationship …
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The eIF2 phosphatase: characterization and modulation
… fulfilled by the combined activity of functional proteins. Consequently, cells are equipped with a complex proteostatic network that controls protein production in response to cellular requisites. Thereby, protein synthesis is induced or attenuated depending on the particular cellular conditions. …
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The role of Cdc7 and cyclin-dependent kinases in DNA replication and S phase
… the genome and requires the activities of two protein kinases, S phase-Cdk and Cdc7. In this thesis, I present work relating to the role of the S phase-promoting kinases in DNA replication and S phase regulation. <br/><br/>Using the cell-free system of Xenopus egg extracts, a small molecule …
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Quantitative Analysis of the Structural Dynamics of Mitotic Chromosomes in Live Mammalian Cells
… were used to probe the role of PNUTS, a major protein phosphatase 1 nuclear-targeting subunit, in the regulation of chromatin structure. In live cells depleted of PNUTS by RNAi, compaction was slowed at least 3-fold. Our collaborators in the group of Philippe Collas at the University of Oslo …
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Towards understanding substrate recruitment by PP1 holophosphatases
Protein phosphorylation is a vital regulatory mechanism within the cell which is commonly dysregulated in diseases such as cancer and neurodegeneration. The addition of a phosphoryl group to a protein is catalysed by a group of enzymes known as kinases and the removal is catalysed by phosphatases. …
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Characterization of a Novel Chromatin-Induced Mechanism that Couples Microtubule Disassembly and Nuclear Re-Formation
… Cdc48/p97, which physically extracts the protein from chromatin at anaphase. Aurora B is the catalytic subunit of the Chromosome Passenger Complex (CPC), which promotes microtubule polymerization and spindle formation from mitotic chromosomes. Removal of the CPC from chromosomes at …
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Structural and functional characterisation of pUL21, an α-herpesvirus phosphatase adaptor protein
… Virus infection is a complex process where viral proteins cooperate to exploit host cell metabolism while evading immune responses. Herpes simplex virus 1 (HSV-1) is a very prevalent human herpesvirus that causes life-long infection and is known to dramatically remodel the cellular environment …
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Regulatory Factors of Cellular Senescence in Human Diploid Fibroblast : SA-p-Erk1/2, PP1/2A, PKCα
… shapes, and increase of cell cycle regulator proteins are well-known characteristics of cells entered into senescence. Extracellular signal-regulated kinase 1/2 (Erk1/2) is one of the mitogenactivated protein kinase (MAPK). Erk1/2 signaling promotes cell proliferation and cell surnival. …
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Spinophilin Signaling: Impacts on Body Weight, Obesity, and Beta-Cell Function
… Spinophilin is a filamentous (F)-actin binding, protein scaffolding, and protein phosphatase 1 (PP1)-targeting protein that can regulate protein. Spinophilin has multiple actions. Spinophilin can bundle filamentous actin to modulate the cellular cytoskeleton. Spinophilin also mediates substrate …
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