Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"protein patterning"”.
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Probing surface protein patterning in biological systems using fluorescence nanoscopy
… we applied fluorescence nanoscopy to study the patterning of proteins in biological surfaces at a high spatial resolution of 25 nm. We also developed new analytical methods to extract quantitative information on protein arrangement within biological substrates to elucidate key phenomenon such as …
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Preparation And Characterization Of Self-Assembled Monolayers And Mesoscale Protein Patterning
… to generate a carboxylic acid group. Lysozyme protein was immobilized on the polar carboxylic acid group. The developed protein patterns were investigated using fluorescence microscopy. Lysozyme has an isoelectronic point of 11.35. At a pH below this value the protein is positively charged …
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Design and development of a site specific protein patterning technique for use in a microfluidic antibody separation device
… surfaces presenting multiple micron scale protein motifs, for integration within a microfluidic channel device, is described in this thesis. <br/><br/>Initial research focussed on conjugating protein motifs on silicon <100> substrates in micron and submicron scale patterns. A method …
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Mechanochemical pattern formation in the cellular actomyosin cortex
Protein patterning is essential for cellular function. From cell division to cell migration, specific positional and temporal arrangement of proteins are requisite to triggering and executing vital cell processes. In the aforementioned examples and many other fundamental cellular activities, …
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Immunological synapse arrays : patterned protein surfaces that modulate immunological synapse structure formation in T cells
… this question, we fabricated multicomponent protein surfaces that surrogate the role of APCs and studied T cell responses as a function of synapse structure. To pattern multiple proteins on surfaces, we synthesized and characterized a new polymer, poly(o-ntrobenzyl methacrylate-r-methyl …
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Novel blood platelet diagnostic platform
… of platelets in micrometer sized area using a protein patterning technique makes the analysis innovative and potentially very useful.
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Molecular-Level Investigations Combining Nanoscale Lithography and Atomic Force Microscopy
… nanostructures of OTS were used as a resist for patterning fibronectin, an extracellular matrix protein. Particle lithography provides a practical and reproducible approach to generate billions of nanostructures comprised of organic thin films or nanomaterials. A film of mesospheres can be …
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Topology, Symmetry and Mechanics: Deciphering and Controlling information flows in a living cell
… Take a living cell for instance, the signaling proteins are capable of forming patterns on lengths that are tens of thousands the molecular size. During force-generating processes such as cell divisions, both the spatial and temporal aspects of protein patterning convey essential physiology …
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Synthetic In Vivo Reconstitution of Asymmetric Cell Division
… is known to be controlled, at least in part, by protein complexes that reside asymmetrically at the cortex of dividing cells. These asymmetric domains are involved in orienting the division plane, localising proteins important for cell fate, as well as for generating polarity in cytosolic …
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Development and application of optical microscopy tools for the study of axon guidance
… are initiated, one of which induces local protein synthesis (LPS), a key process to enable rapid navigational responses to guidance cues. Impairments of axon guidance and LPS are associated with several neurological disorders. In this thesis, state-of-the-art optical microscopy-based tools …
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Patterning planar surfaces with motor proteins: Towards spatial control over motile microtubules
… work was to pattern planar surfaces with motor proteins in a way that allows controlled and guided movement of microtubule-shuttles. The first part of the work was in particular focused on generating nanometer–sized tracks of motor proteins on unstructured surfaces. Specifically, microtubules …