Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"protein nmr"”.
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Automated protein NMR data analysis and its application to a-synuclein fibrils
In principle, nuclear magnetic resonance (NMR) spectroscopy provides structural and conformational information with sub-Angstrom precision and the ability to measure dynamics with timescales ranging from femtoseconds to years, all with atomic specificity. However, due to the relatively low …
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Relationships Between Physical, Chemical, and Functional Properties of Enzymatically-Modified and Unmodified Soy Protein (Nmr, Plasteins, Denaturation, Extrusion)
… of enzymatically-modified and unmodified soy protein isolate (SPI). Systems were analyzed for average molecular weight (AMW), TCA and water solubility, functionality, process performance, (twin-screw extrusion), and high-resolution pulsed proton magnetic resonance (PMR). The effects of solvent …
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Molecular Level Interaction of Human Fibroblast Growth Factor-1 (hFGF-1) With Phloridzin
… of growth factors which includes twenty three proteins. FGFs work as modulators for various cellular activities like mitosis, differentiation and survival. Among the FGF family, human fibroblast growth factor-1 (hFGF-1), which is also known as acidic fibroblast growth factor, is a potent …
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Proteins Inside Cells and On the Surface of Membranes: Developing Three Dimensional In-Cell NMR and Structural Characterization of a Trimeric Membrane Proximal External Region Construct from HIV-1 gp41
… to determine atomic resolution structures of proteins. These advancements have also enabled new ways to study difficult protein systems. In this dissertation, I will discuss our efforts to apply NMR spectroscopy and other biophysical techniques to better understand two problems. The first …
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Functional Interactions of E2~Ubiquitin Conjugates in and outside the Ubiquitination Pathway
… (E1, E2, E3), the molecular details required for protein ubiquitination are largely unknown. To address questions regarding the final stages of Ub transfer, we began with structural characterization of the pathway intermediate, the E2~Ub conjugate (~ indicates the thioester linkage between the E2 …
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Adapting Proteins for Clinical and Industrial Use
… to pharmaceuticals to biofuel production. Protein engineering is used to make enzymes more readily available, to gain new or improved catalytic function, or optimize properties such as thermostability and enantioselectivity. In this thesis we will discuss the variety of ways proteins can be …
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Structural and mechanistic investigation towards the regulation of M.tb isocitrate lyase
… of action is not known. In this work, by using protein X-ray crystallography, mass spectrometry and mutagenesis, it was demonstrated that itaconate binds covalently to the active site cysteine of both M. tb ICL1 and ICL2. In addition, it was found that the presence of the ICL reaction product, …
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MOLECULAR MECHANISMS OF ATP-MODULATED LIQUID-LIQUID PHASE SEPARATION (LLPS) OF TDP-43 AND SARS-COV-2 NUCLEOCAPSID PROTEIN
… to behave as a biohydrotrope to prevent protein LLPS. However, a high-resolution mechanism for ATP-modulated LLPS has been lacking so far. Here we included TDP-43 and SARS-CoV-2 N proteins as two paradigms to decipher how ATP regulates protein LLPS. The results revealed that ATP …
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Specific Binding Affinity of the Non-Catalytic Domain of Eukaryotic Like Type IB Topoisomerase of vaccinia Virus
<p>Topoisomerases are ubiquitous proteins that alter supercoiling in double stranded DNA (dsDNA) during transcription and replication and. <em>vaccinia</em> and the closely related poxvirus <em>variola</em> virus, at 314 amino acids in length, encode the smallest of the type I …
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HIV-1 Vpu Oligomerization in Membrane Mimetics
… into a manageable chronic condition. Among other proteins, HIV-1 encodes the Vpu accessory protein which upregulates the process of viral release from infected cells, providing an enticing target for future anti-HIV-1 therapies. In this thesis, I outline a conserved AxxAxxxAxxxA motif responsible …
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Development and Application of 19F NMR of Proteins
19F NMR studies of proteins provide unique insight into biologically relevant phenomena such as conformational fluctuations, folding and unfolding, binding and catalysis. While there are many advantages to the use of 19F NMR, experimental challenges limit its widespread application. The focus of …
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NMR Studies of the RNA Binding Domain of U1A protein and its Complexes with RNA
Eukaryotic splicing is catalysed by five RNA protein complexes called snRNP's. U1A is one protein component of the U1 snRNP particle. The role of U1A in splicing is unclear, but it does bind a hairpin loop from U1 RNA. U1A also binds two internal bulges found in the 3' untranslated region of the …
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Investigating the C-terminal region of RalA: regulation through membrane dynamics and protein-protein interactions
… there is a growing need to understand these proteins in terms of their structure and function to facilitate future pharmaceutical intervention in this critical oncogenic pathway. So far, biophysical characterisation of Ral proteins has typically been carried out with truncated constructs that …