Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"protein microarray"”.
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Identification of surface protein complexes of Streptococcus pyogenes through protein microarray technology
… comprehensive understanding. The knowledge of protein complexes composition and structure could offer new, more effective targets for a more specific and consequently effective immune response against a complex instead of a single protein. Large-scale protein-protein interaction screens are the …
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P450 biochips : development of a protein microarray platform for investigating cytochrome P450 clinical drug metabolism
This thesis describes the development of a novel cytochrome P450 array format, the P450 Biochip that allows quantitative and truly high-throughput measurement of cytochrome P450-mediated turnover reactions in sub-nanolitre volumes.
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Development and application of an antibody-based protein microarray to assess stress in grizzly bears (Ursus arctos)
… The objective of this work was to develop a protein microarray that could detect patterns of physiological stress in a rapid manner with small samples of grizzly bear tissue. Sampling from four regions in the foothills of the Rocky Mountains in Alberta resulted in the capture of 133 bears. …
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Statistical models with diverging dimensionality
… qualification procedure for lysate protein microarray; ii) theoretic analysis for one-way ANOVA with diverging dimensionality and iii) statistical analysis for multi-task learning.
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Proteomic studies on patient responses to chemotherapy, radiotherapy and immunotherapy in cancers
… (CT) antigens - a family of ca. 150 proteins that are both autoimmunogenic and mainly restricted to tumours in various types of human cancers - makes them attractive immunotherapy targets, as well as possible cancer diagnostic markers. We carried out a retrospective serological study …
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A B-cell or a key player?: The role of the humoral immune response in melanoma, highlighting the importance of method development
… healthy controls (n=40) were screened on a human protein microarray to detect IgG reactivities. Associations between these data and gene expression, tumour mutation burden, MHC types and protein subcellular location were explored in relation to potential drivers of autoantibody production. The …
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Fluorescence resonance energy transfer study of the global folding of functional DNAs and electrohydrodynamic printing of protein arrays
… jet (E-jet) printing is applied to the protein microarray field. With the development of multi-nozzle printing system, both single-protein array and multiple-protein arrays are successfully demonstrated. Several proteins, such as streptavidin, Green fluorescence protein (GFP), mCherry (a …
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Functional characterization of poly(ADP-ribose) dependent ubiquitin E3 ligase in the DNA damage response
… in the DNA damage response 8 4. Heterochromatin protein 1 functions in the DNA damage response 9 D. Poly(ADP-ribosyl)ation mediated DNA damage response signaling 10 1. DNA damage induced PARP1 activation 10 2. Free pADPr polymer function in DNA damage response 11 E. Aim of this study 12 Ⅱ. …
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Improved microarray sensitivity using photonic crystal enhanced fluorescence
… quantify relevant biomolecules such as DNA and proteins. Microarrays in particular have enabled researchers and clinicians to monitor gene expression and protein concentrations in a high-throughput fashion, providing valuable information about cellular and systemic function. Despite the …
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Multiplex-Serologie - Simultane Analyse von Antikörper-Antworten gegen humane Papillomviren (HPV) mit Protein-Arrays
… jedoch durch die Vielfalt der Virustypen und -proteine und die Typspezifität der gegen sie gerichteten Antikörper erschwert. Der bisher zur Detektion von Antikörpern genutzte GST capture ELISA ist methodisch ausgereift und gut validiert, im Hinblick auf die Zahl parallel analysierbarer Antigene …
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NF-kB Inducing Kinase (NIK) Influences Eosinophil Development, Survival, and Plasticity
… cell selection, MTT assay, BrDU assay, and protein microarray analysis. Overall, our findings suggest that NIK influences eosinophil maturation, proliferation, metabolism, survival, and potentially plasticity in vivo and in vitro under different environmental conditions and Th2 cytokine …
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Fic-Mediated AMPylation in Bacterial Infection and Endoplasmic Reticulum Stress
… addition of an adenosine monophosphate to a protein resulting in a modified protein with altered activity. Proteins capable of catalyzing AMPylation, termed AMPylators, are comparable to kinases in that they both hydrolyze ATP and reversibly transfer a part of this primary metabolite to a …
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Multi-functional, self-sensing and automated real-time non-contact liquid dispensing system
… of researches on the deoxyribonucleic acid (DNA) microarray, compact and low-cost direct write technology (DWT), organic semiconductors and nano-particles. The existing approaches, commercialized or experimental, to liquid dispensing in minute amounts have one common shortcoming: open loop …
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Next generation instrumentation for photonic crystal biosensors: a passage to early detection of cancer
… enhancement as well as quality control for a protein microarray, helping to identify discrepancies in binding densities of antibodies. The angle-scanning technique coupled with the new modality shows a significant reduction in the coefficient of variation by 20-99% compared to ordinary …
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Autoantibody Responses to Traumatic Brain Injury
… papers have described autoantibodies to brain proteins occurring following TBI. This thesis describes the development of a protein microarray approach for screening serum for autoantibodies following TBI, and uses this to investigate the autoantibody responses which occur after TBI, both during …
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Exploring the effects of polymorphic variation on the stability and function of human cytochrome P450 enzymes in silico and in vitro
… calculations on the effect of mutations on protein stability in order to establish the likely causes of altered drug metabolism observed for cytochrome P450 variants in functional assays carried out to date. This study revealed that 75% of all cytochrome P450 mutations showing altered …
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Application of silicon photonic microring resonators to the study of surface bioconjugation and protein capture agent binding kinetics
… estimated one million functionally distinctive proteins, a means for high-throughput analysis, such as protein microarray, is required for the identification and quantification of proteins from biological samples. Even though protein microarrays have been shown to be a powerful technique for …
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Role of Phosphorylation In The Regulation of Prmt5
<p>PRMT5 is a member of a group of proteins that mediate arginine methylation. It is involved in diverse cellular processes, including cell differentiation, splicing, transcription elongation and epigenetic silencing, and its expression is dysregulated in many cancers. Due to its pleiotropic …
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Epigenetic Regulation of Skin Development and Postnatal Homeostasis The role of chromatin architectural protein Ctcf in the control of Keratinocyte Differentiation and Epidermal Barrier Formation
… described previously. Chromatin architectural protein Ctcf has previously been implicated in the control of long-range enhancer-promoter contacts and inter-chromosomal interactions. Ctcf is broadly expressed in the skin including epidermal keratinocytes and hair follicles. Conditional Keratin …