Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"protein immobilization"”.
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NON-FOULING AFFINITY PLATFORMS FOR PROTEIN IMMOBILIZATION IN ELECTRON MICROSCOPY
… substituents that promotes specific capture of protein targets for high resolution TEM analysis. The utilization of these grids for specific adsorption of the targeted protein onto the grid surface results in well-controlled surface concentration enhancements and a days-to-minutes reduction in …
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Protein immobilization using complex coacervates and complex coacervate thin films
… often be converted to heterogeneous catalysts. Protein immobilization enables this conversion and can enhance the stability of enzymes. Complex coacervates are highly effective at encapsulating and stabilizing enzymes. This thesis demonstrates the use of complex coacervate thin films for the …
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FTIR-ATR Characterization of Hydrogel, Polymer Films, Protein Immobilization and Benzotriazole Adsorption on Copper Surface
… groups are used as solid surface for the immobilization of bovine serum albumin (BSA). Pulsed plasma polymerization was used to polymerize these monomers with different duty cycles. Initial works in this field were all about protein surface adsorption. But more recently, the emphasis is on …
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Effects of Immobilization Matrices and Protein Orientation on Biomolecular Recognition at Solid-Liquid Interfaces
… to affinity chromatography. However, the immobilization of biomolecules, such as proteins, often results in the loss of their biological activities. Factors that impact the apparent kinetics and affinities of the biomolecular interactions at the solid-liquid interfaces were examined with a …
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Engineering functional biomaterials to advance environmental and resource sustainability
… into recoverable forms. In recent years, protein-based biosorption and biocatalysis have emerged as promising alternatives to conventional methods due to their inherent advantages, such as strong substrate affinity, selectivity, rapid adsorption kinetics, potent enzymatic activity, and …
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Elastin-like polypeptide (ELP) tags for self-assembly and high-throughput processing of functional protein materials
… and catalytic capabilities of globular proteins makes these biomolecules promising candidates in a broad array of applications, including industrial production of commodity chemicals, point-of-care diagnostics, and therapeutics. Self-assembly of globular protein-polymer bioconjugates …
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Optimizing Acyclic Identification of Aptamers
… method that eliminated the requirement for protein immobilization was developed. Reversible formaldehyde cross-linking in conjunction with Electrophoretic Mobility Shift Assay was used to successfully identify TBA above background in proof of concept experiments using amplification free …
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Miniature fiber-optic multicavity Fabry-Perot interferometric biosensor
… of self-assembled thin films, receptor immobilization process or biological reaction is limited in the FFPI technique due to the difficulty of forming Fabry-Perot cavities by the thin film itself. Novel methods are needed to provide an accurate and reliable measurement for monitoring the …
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Proteome-Seq: Sequencing-Based Readout Of Proteomic Analytical Assay
… analysis, it captures cellular states pre protein translation. Additionally, cell RNA abundance correlates poorly with protein abundance, thus explaining only fraction of protein variability within the cells. Proteins, primary drivers of cellular functions and processes, exhibit great …
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Biocatalysis on nanostructured surfaces : investigation and application of redox proteins using spectro-electrochemical methods
… different aspects within the research field of protein spectro- and electro-chemistry on nanostructured materials are addressed. On the one hand, this work is related to the investigation of nanostructured transparent and conductive metal oxides as platform for the immobilization of …
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Fabrication and characterisation of ZnO nanostructures: from nanoscale building blocks to hybrid nanomaterials - towards emerging technologies in sensing applications
Metal oxide nanostructures characterized by multiple morphologies and structures are at the forefront of applications driven nanotechnology research. In particular, they represent a versatile solution for performance enhancement and applications in multifunctional devices and offer distinct …
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NOVEL MICROTUBULE TARGETING AGENTS: SYNTHESIS AND STRUCTURAL DETERMINATION OF THEIR INTERACTIONS
… they could be used e.g., to immobilize the protein of interest, tubulin. In Section 2.3, the synthesis of simplified biotinylated pironetin analogues is described. Pironetin, a natural product that covalently binds alfa-tubulin, and the possibility of its biotinylation to use then biotin …
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Preparation and characterization of functionalized surfaces for desorption-ionization mass spectrometry
… of the three-dimensional structures of proteins, as well as their dynamics. However, Current standard practices require a large number of separate processing steps, especially when the structural data is obtained from the bottom-up approach. Moreover, these steps are mostly performed at …