Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 60 for “"protein identification"”.
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Implementation of nano-liquid chromatography hyphenated to tandem mass spectrometry for protein identification in gel and non-gel based proteomics
… of large scale strategies for the analysis of proteins in biological systems. In Chapter I, genomics and the need to shift towards proteomic approaches are outlined. An overview of key technologies used in functional and structural proteomics is provided in Chapter II, whereas mass spectrometry …
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Computational Analysis of Antibody Binding Mechanisms to the Omicron RBD of SARS-CoV-2 Spike Protein: Identification of Epitopes and Hotspots for Developing Effective Therapeutic Strategies
… receptor binding domain (RBD) of the Spike protein. The study demonstrates that the Omicron variant harbors 23 unique mutations across the RBD regions I, II, III, and IV. Of these mutations, seven are shared between RBD regions I and II, while three are shared among RBD regions I, II, and …
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RAMIE (BOEHMERIA NIVEA): AN UNDERUTILIZED PLANT FOR A MULTIPURPOSE INDUSTRIAL APPLICATION; A PERENNIAL PLANT WITH PROTEIN IDENTIFICATION USING MALDI-MS, A BIOSORBENT FOR Hg (II) REMOVAL FROM WATER AND AS A BAST FIBER PRODUCTION VIA ENZYMATIC DEGUMMING
… using a proteomic method to investigate unknown proteins in the leaves of ramie and to study their benefits to the biological system. PPiase and RuBisCO with molecular weight 15 and 52 KDa were identified as the proteins after peptide mass fingerprinting analysis in mascot based based on their …
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Homology-Based Functional Proteomics By Mass Spectrometry and Advanced Informatic Methods
… characterization of biochemically-isolated proteins is a central task in the biochemical and genetic description of the biology of cells and tissues. Protein identification by mass spectrometry consists of associating an isolated protein with a specific gene or protein sequence in silico, …
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A Bayesian framework for statistical signal processing and knowledge discovery in proteomic engineering
… Bayesian framework in mass-based proteomics for protein identification. Using the Bayesian framework in a statistical signal processing manner, mass spectrometry data is filtered and analyzed in order to estimate protein identity. This is done by a multi-stage process which compares probabilistic …
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Machine learning for cryo-EM structure determination
… on ModelAngelo, an automated model-building and protein-identification program for cryo-EM. I designed specialised graph-neural-network architectures that allow ModelAngelo to build atomic models in high-resolution maps with accuracy matching that of human experts. I also developed a …
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Chemical and enzymatic tools to study proteins in their native cellular environment
… trafficking, and turnover of individual proteins, strategies that allow the tagging, and imaging, and identification of entire proteomes have also offered valuable insights into disease biology. Since protein visualization serves as a complement to protein identification, this thesis …
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Investigating Protein Properties via Microfluidic Techniques
Proteins are one of the most abundant biomacromolecules and play important roles in bioactivities within organisms, including key functions in DNA replication, immune system response, metabolic reactions and molecular transportation. Proteins fold into their unique compact three-dimensional …
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Database support for top-down proteomics
… is a revolutionary application for the identification and characterization of protein, known to be one of the most complicated and challenging issues in biology. In top-down proteomics, the quality and speed of the data warehouse is very important, as high accuracy results are returned …
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Subunit Compositions of Arabidopsis DNA-Dependent RNA Polymerases and the Roles of the Plant-Specific RNA Polymerases IV and V in Gene Silencing
… Using a combination of affinity purification and protein identification by mass spectrometry, my studies show that Arabidopsis Pol IV and Pol V are composed of subunits that are paralogous or identical to the twelve subunits of Pol II. Four subunits of Pol IV are distinct from their Pol II …
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The TAZ Protein Interactome in Striated Muscle
… ability, TAZ function exclusively depends on its protein:protein interaction network. This prompted us to undertake a proteomic-based study aimed at identifying the TAZ interactome in striated muscle cells. Using a novel GFP-Nanotrap based affinity purification approach coupled with LC-MS/MS …
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Overexpression of active AKT3 induces differential binding of coregulator proteins to the estrogen receptor as a possible mechanism of Tamoxifen resistance
… attributed to binding of different co-regulator proteins and/or different binding affinity of these proteins to the estrogen receptor in M CF-7 cells overexpressing active Akt3 as compared to parental MCF-7 cells. We have immuno-precipitated the estrogen receptor along with bound co-regulator …
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Kriauklių proteomika: Unionida (Bivalvia, Mollusca) kriauklių baltymų analizė rūšių identifikavimui /
… times, scientists decided to analyze shell proteins. To identify the proteins in the shells, scientists are doing paleoproteomics which uses mass spectrometry and bioinformatic programs. In this work, two freshwater unionoid were used: Unio tumidus and Anodonta anatina shells. The aim of the …
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An Apache Hadoop Framework for Large-Scale Peptide Identification
<p>Peptide identification is an essential step in protein identification, and Peptide Spectrum Match (PSM) data set is huge, which is a time consuming process to work on a single machine. In a typical run of the peptide identification method, PSMs are positioned by a cross correlation, a …
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A hair bundle proteomics approach to discovering actin regulatory proteins in inner ear stereocilia
… pursue a proteomics-based approach to discover proteins that play a role in stereocilia function. I employed a modified "twist-off" technique to isolate hair bundle proteins, and I developed a method to purify proteins and to process them for analysis using multi-dimensional protein …
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Changes in the Rpb3 Interactome Caused by the Deletion of RPB9 in Saccharomyces cerevisiae
… genes. Pol II is a complex composed of twelve protein subunits. This study focused on the changes in the interactome of Rbp3 in S. cerevisiae when the Pol II subunit Rpb9 is removed. Rpb3 is one of the core subunits of Pol II, and any significant changes to the Rpb3 incteractome due to the loss …
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Machine learning for understanding protein sequence and structure
Proteins are the fundamental building blocks of life, carrying out a vast array of functions at the molecular level. Understanding these molecular machines has been a core problem in biology for decades. Recent advances in cryo-electron microscopy (cryoEM) has enabled high resolution experimental …
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Distributed Approach for Peptide Identification
<p>A crucial step in protein identification is peptide identification. The Peptide Spectrum Match (PSM) information set is enormous. Hence, it is a time-consuming procedure to work on a single machine. PSMs are situated by a cross connection, a factual score, or a probability that the match between …
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The Proteomic Response of Gill Tissue in Tidally and Subtidally-Acclimated California Mussels, Mytilus californianus, to Acute Emersion-Induced Anoxia
… collected gill tissue for subsequent analysis of protein abundance with 2D gel electrophoresis and protein identification with tandem mass spectrometry. Relative to subtidally-acclimated mussels, tidally-acclimated mussels showed a greater propensity to respond to distrupted protein homeostasis …
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Modulation of protein expression in Mycobacterium tuberculosis-stimulated TB- IRIS patient-derived PBMCs by omega n-3 polyunsaturated fatty acids
… shotgun proteomics. MaxQuant was used for protein identification and Perseus for statistical analysis. Stimulation with Mtb WCL shifted the secretome of healthy PBMCs towards an inflammatory state, and this was altered by treatment with EPA/DHA via changes in the regulation of several …
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