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Showing 1 to 6 of 6 for “"protein hydration"”.
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PROTEIN HYDRATION DEPENDENCE OF THE AMIDE HYDROGEN EXCHANGE OF LYSOZYME.
… solution samples and powder samples at varied hydration levels for pH 2, 3, 5, 7 and 10. The dependence of exchange of powder samples on the degree of hydration was the same at all pH, reaching rates of exchange equivalent to solution samples at 0.2 g H₂0/g protein (160 mol H₂0/mol protein), …
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Dynamics of biopolymers and their hydration water studied by neutron and X-ray scattering
Protein functions are intimately related to their dynamics. Moreover, protein hydration water is believed to have significant influence on the dynamics of proteins. One of the evidence is that both protein and its hydration water have the same dynamic transition temperature at around 220 K. This …
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Comparative characterisation of esterases and lipases for biosensors
… in the elucidation of the relationship between protein hydration and gas phase activity. This involved comparison of appropriate kinetic parameters against the esters ethyl acetate, methyl butyrate and ethyl butyrate and their equivalent p-nitrophenylated synthetic model substrates. Comparison …
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Structure, Function and Dynamics of G-Protein coupled Receptors
Understanding the function of membrane proteins is crucial to elucidate the molecular mechanisms by which transmembrane signaling based physiological processes,i. e., the interactions of extracellular ligands with membrane-bound receptors, are regulated. In this work, synthetic transmembrane …
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Neutron scattering investigations on the liquid-liquid transition in deeply cooled confined water
… HDL in the confined water. Water is crucial to protein dynamics. We investigate the role water plays in sub-picosecond collective vibration of protein with inelastic X-ray scattering and find that the hydration water makes the intraprotein longitudinal phonons "softer". We also study the slow …
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The Effect of Hydration on Enzyme Activity and Dynamics
… the molecular basis of the role of water in protein function, several studies have established a correlation between enzyme activity and hydration level. While a threshold of hydration of 0.2 h (grams of water per gram of dried protein) is usually accepted for the onset of enzyme activity, …