Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 32 for “"protein glycosylation"”.
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Protein glycosylation in the gram-negative gamma proteobacterium photorhabdus luminescens
… that Photorhabdus luminescens produces glycoproteins and thus contains a protein glycosylation system. P. luminescens is a pathogen of insects and a symbiont of soil nematodes. Adhesion and invasion are very important in the life cycle of the organism and it is speculated that the bacteria …
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Investigating asparagine-linked protein glycosylation in eukaryotic and prokaryotic systems
N-linked protein glycosylation is characterized by the formation of a -glycosylamine linkage to an asparagine residue within the Asn-Xaa-Ser/Thr consensus sequence. This modification is found in organisms from eukaryotic, archaeal and bacterial domains and is implicated in numerous cellular …
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Protein glycosylation by NleB and the secretion of therapeutic nanobody fusions
… glycosyltransferases. They glycosylate host protein substrates on arginine residues with N-acetyl glucosamine (GlcNAc) to inhibit the function of host proteins involved in the innate immune response. Originally, it was thought that the effectors are inactive within the bacterium and fold into …
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NEW METHODS TO QUANTIFY METHIONINE OXIDATION AND PROTEIN GLYCOSYLATION AND A DE NOVO GLYCOPEPTIDE DECOY GENERATOR
Protein post-translational modifications (PTMs) produce complex, heterogeneous species that can best be identified and analyzed using mass spectrometry (MS). The ever-growing number of protein bioetherapeutics need effective MS tools to characterize PTMs and ensure drug safety and efficacy. …
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Biochemical characterization of Cj 1123 c, a putative acetyltransferase & Cj 1319, a putative C6 dehydratase from Campylobacter jejuni.
Glycoproteins are important for the virulence of Campylobacter jejuni. The enzymes involved in protein glycosylation could provide new therapeutic targets. Two such proteins are Cjll23c, a putative acetyltransferase and Cj 1319, a putative GDP-mannose dehydratase encoded by the N- and O-linked …
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Elucidation of the pathways responsible for the biosynthesis of UDP-N,N'-diacetylbacillosamine in bacterial pathogens
… microbes through its incorporation onto various protein virulence factors. In particular, diNAcBac is found at the reducing end of glycans in both asparagine (N-linked) and serine/threonine (0-linked) protein glycosylation pathways. The second and third chapters examine the O-linked protein …
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Protein expression and glycosylation in CHO cells
… depends on sufficient expression and correct glycosylation of the recombinant product. Low product titer and inconsistent protein glycosylation constitute two major problems frequently encountered in biopharmaceutical production. Using Chinese Hamster Ovary (CHO) cells expressing recombinant …
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Characterization of Glycans Derived from Biological Samples Using LC-MS/MS
Proteins are intricate compounds that play vital roles in biological activities. One of the most prevalent post-translational modifications (PTMs) is protein glycosylation, which enhances several biological functions, such as protein stability, localization, cellular communication, inflammation, …
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Unnatural glycopeptides with application on cancer research
Protein glycosylation is the most frequent post-translational modification which mediates a variety of cellular processes. In addition, changes in protein glycosylation can modulate cellular phenotypes such as growth, development, and disease. Therefore, the main objective of the present PhD …
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Polyprenyl-dependent glycan assembly pathways in microbial pathogens
… of the undecaprenyl-dependent O-linked protein glycosylation pathway in Neisseria gonorrhoeae. The N. gonorrhoeae pathway is shown to produce UDP-N,N'-diacetylbacillosamine, which is the UDP-sugar donor in the first membrane-associated step. Furthermore, it is demonstrated that …
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Structural underpinnings of membrane association and mechanism in the monotopic phosphoglycosyl transferase superfamily
In prokaryotes, protein glycosylation can be a determinant of pathogenicity as it plays a role in host adherence, invasion, and colonization. Impairment of glycosylation in some organisms, for example N-linked glycosylation in Campylobacter jejuni, leads to decreased pathogenicity; thus, opening …
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IMPROVING GLYCOSYLATION EFFICIENCY IN ESCHERICHIA COLI BY MAMMALIAN-CELL-INSPIRED PROTEIN-PROTEIN INTERACTIONS
Asparagine linked glycosylation (N-glycosylation) is one of the most common post-translational modifications found in proteins. The central enzyme in the N-glycosylation pathway responsible for the transfer of glycans onto asparagine residues is called the oligosaccharyltransferase (OST). In …
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Development of novel conjugate vaccines against Salmonella
… the pathogen, chemically coupled to a carrier protein. Several clinical trials have shown that glycoconjugates are promising vaccine candidates to prevent Salmonella infections. However, manufacturing of conjugate vaccines is a complex, multi-step process. An alternative approach to produce …
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ENGINEERING WATER-SOLUBLE VARIANTS OF THE SINGLE-SUBUNIT OLIGOSACCHARYLTRANSFERASE
… a key enzyme in the asparagine-linked (N-linked) protein glycosylation pathway. OSTs exist in all domains of life and are capable of transferring a preassembled glycan from lipid carrier to an acceptor peptide. Bacterial OSTs are an single-subunit enzyme that are amenable to recombinant expression …
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Functional analysis of glycosylation of Zika virus envelope and NS1 proteins
… syndrome. ZIKV has two important glycoproteins, both the envelope (E) protein, and nonstructural protein 1 (NS1). The ZIKV envelope (E) protein is responsible for viral entry and represents a major determinant for viral pathogenesis. NS1 forms a homodimer necessary for viral replication …
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Functional analysis of glycosylation of Zika virus envelope and NS1 proteins
… syndrome. ZIKV has two important glycoproteins, both the envelope (E) protein, and nonstructural protein 1 (NS1). The ZIKV envelope (E) protein is responsible for viral entry and represents a major determinant for viral pathogenesis. NS1 forms a homodimer necessary for viral replication …
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Studies towards the in vivo inhibition of oligosaccharyl transferase
Protein glycosylation is an important process because of the great diversity of glycoproteins that can be produced by the introduction of different oligosaccharide sequences. Our group has made significant progress in the study of asparagine-linked glycosylation. This process is catalyzed by …
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Assembly and Display of Surface Proteins In Actinomyces Oris
… oris</em> utilizes cell wall anchored proteins and glycoconjugates to initiate adherence to host surfaces, recruit additional bacterial species that could not bind otherwise, and maintain the structural integrity of the oral biofilm. In this thesis, I reveal mechanisms involved in the …
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Structural Analysis of Carbohydrates by Mass Spectrometry
<p>Protein glycosylation is a highly frequent post-translational modification. Glycosylation is actively involved in intermolecular and intercellular binding events that are important to a wide range of biological functions such as immunity and fertility. The unique functions of glycans are …
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Complex N-Glycosylation in Anti-Tumor Immunity
… potential anti-tumor target for two reasons: (i) protein glycosylation is known to play a role in tumor progression, and (ii) alternatively glycosylated proteins may function as tumor neoantigens. The glycosyltransferase Mgat5 catalyzes the formation of 1,6-N-acetylglucosamine branched glycans, …
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