Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"protein fusions"”.
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Engineered [beta]TCP-binding HER-family protein fusions and their use for improving osteoprogenitor- mediated bone regeneration
Autologous bone marrow grafting has been shown to aid in the healing of bone defects since the 1950s. Transplantation of freshly-aspirated autologous bone marrow, together with a scaffold, is a promising clinical alternative to harvest and transplantation of autologous bone for treatment of large …
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Epigenetic and post-transcriptional regulation of gene expression in pluripotent stem cells, differentiation and metastasis
Transmission of information from DNA to RNA to protein underlies the core of modem life forms. The advance in sequencing and genetic technologies has revolutionized the study of molecular biology, genetics and developmental biology enabling delineation of biological processes in unprecedented …
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Structure-property relationships in physical polymer gels
… Above the critical gel concentration, these protein fusions form disordered spherical nanostructures in solution. In shear flow, the rate of network deformation and rearrangement could be increased or decreased by changing block architecture, temperature, or concentration of the system. …
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The streptomyces cytoskeletal protein (Scy) is a key component of the tip organising centre for polarized growth in streptomyces coelicolor
… homologues as well as a number of accessory proteins unique to prokaryotes. As cell shape determination is dependent on the sites of insertion of new cell wall material, this is characteristically organised and driven by cytoskeletal proteins. Streptomyces coelicolor hyphal growth occurs …
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The G protein-coupling specificity of D2-like dopamine receptors
The G-protein coupling specificity of D2-like dopamine receptors was investigated using both receptor-G protein fusions and membranes of cells in which pertussis toxin-resistant mutants of individual Gi-family G proteins could be expressed in an inducible fashion. A range of ligands displayed …
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Characterisation of the Arabidopsis mid-SUN proteins at the plant NE
The Sad1-Unc84 (SUN) proteins of Arabidopsis thaliana (Arabidopsis) consist of two sub-groups - the classical C-terminal (Cter) SUN domain proteins and the mid-SUN domain proteins. The mid-SUN domain proteins have been suggested to have a functional role in Linker of Nucleoskeleton and Cytoskeleton …
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Directed Migration Achieved in Mammalian Cells via Ca2+ Signal Rewiring in Synthetic Protein Chimeras
… VEGF-A). Here, firstly we engineered fusion proteins that generate a Ca2+ signal upon ligand binding by creating fusions of domains that oligomerize to the transmembrane domain and the cytoplasmic tail of the VEGFR2. By coupling these chimeric proteins that generate Ca2+ signals with proteins …
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Characterisation of the PQ-loop repeat membrane protein family in arabidopsis thaliana
… repeat family, a novel family of membrane proteins from Arabidopsis thaliana. The family consists of six members (AtPQL1-6) all predicted to contain seven transmembrane segments and two copies of the PQ-loop domain. The repeated PQ-loop domain is a well conserved feature present in several …
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Assessing TMV as an immunogenic particle : the expression of HIV-1 subtype C V3 loop neutralizing antibody epitopes on the surface of TMV virions
… were expressed on the surface of the TMV coat protein, which served as an ideal means of antigen display. This model antigen display system was capable of assembling into multivalent, highly repetitive structures thereby displaying many copies of the attached epitope in the assembled virion. …
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Synthesis, nanostructure, and mechanics of thermoresponsively tough biomaterials from artificial polypeptides
Artificial protein hydrogels have attracted interest as injectable fillers and scaffolds for tissue engineering and regeneration, but the same features that enable minimally-invasive implantation of these biomaterials typically make them susceptible to mechanical degradation in the tissue …
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In vivo display: a selection and its derivatives for antimicrobial peptide lead identification
… of random peptides at the terminus of a display protein. A library of 12-amino acid random peptide sequences was added to either the C- or N-terminus of Emerald Green Fluorescent Protein (EmGFP) for the isolation of peptides active in the cytoplasm or the N-terminus of Alkaline Phosphatase (PhoA) …
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Localization of Red Clover Necrotic Mosaic Virus Polymerase Proteins at the Endoplasmic Reticulum of Living Plant Cells
… family. RCNMV encodes N-terminally overlapping proteins of 27 kDa and 88 kDa (p27 and p88). p88 contains motifs characteristic of RNA-dependent RNA polymerases, and while the function of p27 is unclear, it is very likely to be part of the RCNMV replication complex. I have investigated the …