Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"protein fusion"”.
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Assessing TMV as an immunogenic particle : the expression of HIV-1 subtype C V3 loop neutralizing antibody epitopes on the surface of TMV virions
… were expressed on the surface of the TMV coat protein, which served as an ideal means of antigen display. This model antigen display system was capable of assembling into multivalent, highly repetitive structures thereby displaying many copies of the attached epitope in the assembled virion. …
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Identification and Characterization of Novel CRISPR-Cas Immune Effectors
… of three new CRISPR-Cas effector proteins that provide immunity in bacteria in response to phage challenge with highly diverse activities. CRISPR-Cas systems are the only known adaptive immune system found in bacteria. Immunity by these systems is achieved in two main steps: (1) …
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Biochemical Characterization of Two Aminopeptidases Involved in Hemoglobin Catabolism in the Food Vacuole of Plasmodium falciparum
… environment is unique for aminopeptidase P proteins and rare for M1 homologs. Our immunolocalization results have confirmed the dual distribution of these two enzymes in the parasite. Vacuolar targeting was found to be associated with the Plasmodium specific N-terminal extension found in the …
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Elucidation of the Proteases Responsible for and the Form of Soluble, Monomeric EphrinA1 Released From Cancer Cells
… the overexpression of the receptor at the protein level in tumor specimens and cell lines while being absent in normal brain, making EphA2 an attractive target for novel therapeutics and imaging agents. EphrinA1 is a 182-amino acid, GPI-linked ligand for the EphA2 receptor. Activation of …
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Next-generation fluorophores for single-molecule and super-resolution fluorescence microscopy
… fluorescent probes for the study of amyloid protein aggregates implicated in neurodegenerative diseases. This includes a study of the photophysical and binding properties of a novel fluorophore library based on the amyloid dye Thioflavin-T. Following on from this, is the presentation of novel …
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Expanding the Role of Oxidoreductases in Benzylisoquinoline Alkaloid Metabolism in Opium Poppy
… was fused and in frame with a CYP. The resulting protein fusion catalyzed the S-to-R epimerization of reticuline via 1,2-dehydroreticuline. The fusion protein, renamed reticuline epimerase (REPI), was detected in opium poppy and in Papaver bracteatum, which accumulates the morphinan alkaloid …
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Biomolecular condensation of intrinsically disordered regions in P. falciparum and H. sapiens transcription factors
… by intrinsically disordered regions (IDRs) in proteins, has emerged as a previously underappreciated mechanism of cellular compartmentalisation and regulation. The study of eukaryotic transcription has been revolutionised by the understanding that biomolecular condensates formed by …
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Next generation approaches toward engineering therapeutic proteases
… a large excess of disease-associated target proteins in a catalytic fashion. Technological advances in molecular biology have made laboratory-based evolution techniques for protein engineering readily accessible. However, the ability to interrogate the activities and substrate preference of …
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Lipid composition and membrane physical parameters regulate priming and SNARE function in yeast vacuole membrane fusion
Fusion at highly specialized membrane microdomains is a prerequisite for processes like hormone and neurotransmitter release, antigen presentation and viral infection. Apart from a family of highly conserved proteins called SNAREs, membrane fusion also requires a set of regulatory lipids that …
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Investigating Ageing of Neurodegenerative Disease Relevant Protein Condensates
… literature on biological macromolecules such as proteins that can undergo the LLPS process and form biomolecular condensates. It has been demonstrated repeatedly that these condensates can play a wide range of cellular roles, from transcription to metabolism. In addition, an increasing amount of …
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Analysis of Hepatocyte Secretion Pathways: A Case Study on Hepatic Apolipoproteins, Serum Albumin, and Hepatitis C Virus
… many of the liver's essential functions in protein secretion, lipid storage and transport, and excretion. Some of these functions are carried out via polarized secretion of simple protein cargo, such as serum albumin, or large macromolecular lipid-protein complexes, the lipoproteins. The …
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The G protein-coupling specificity of D2-like dopamine receptors
The G-protein coupling specificity of D2-like dopamine receptors was investigated using both receptor-G protein fusions and membranes of cells in which pertussis toxin-resistant mutants of individual Gi-family G proteins could be expressed in an inducible fashion. A range of ligands displayed …
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Understanding Elastin-Like Polypeptide Block Copolymer Self-assembly Behavior
… offer a unique method to display targeting proteins multivalently on micelles to improve tumor cell uptake. As ELPs are genetically encoded, proteins can be seamlessly fused at the genetic level to the ELP diblock. The protein ELP diblock fusions can be synthesized as one polypeptide chain …
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Engineering human single-chain T cell receptors
… scTv and scFv (antibody variable fragments) as fusion proteins, collectively called chimeric antigen receptors, for cell mediated therapies to redirect T cells to specific antigens In chapter 2, two human Valpha2+ T cell receptors specific for human immunodeficiency virus and human T cell …
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Analysis of TpeL secretion in Clostridium perfringens
… secretion of the toxin TpeL by a small membrane protein, TpeE. Preceding the study of TpeL secretion were two other projects, which are discussed in Chapters 2 and 3. Chapter 2 describes an experimental plan to characterize the genes involved in muscle cell adherence as a very basic model to …