Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 66 for “"protein expression levels"”.
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Quantification of the Chemotaxis Protein Expression Levels in Bacillus Subtilis
The expression levels of CheD appear to be tuned to give the functional ratio of protein need to participate in adaptation. When the potential binding partners of CheD are deleted (cheC and all ten receptors), the protein levels of CheD drop significantly. This finding suggests that B. subtilis …
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Molecular characterisation of the lateral organ boundaries gene (XvLOB) from the resurrection plant Xerophyta viscosa
… by in silico analysis, the recombinant XvLOB protein expressed, subcellular localisation examined and both the transcript and protein expression levels in response to dehydration was investigated.
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Optimisation of the mycobacterial replicon of an E. coli-mycobacterial shuttle vector
… repB genes which code for essential replication proteins. Specific aspects of this typical mycobacterial replicon were identified as potential targets to improve stability and increase recombinant protein expression levels, and were modified accordingly.
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The role of Dietary Interventions in Broiler Intestinal Health and Immunity
… capacity, apoptotic and necroptotic cell death levels and the expression of inflammatory cytokines. Supplementation of diet with each mannan-rich fraction (MRF) increased villus height and villus surface area. In addition, crypt depth was increased upon dietary inclusion of naturally-derived …
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In Vivo Investigation of Protein Interactions in Caenorhabditis Elegans by Forster Resonance Energy Transfer Microscopy
… animals expressing CFP- and YFP-fusion proteins. Quantitative FRET microscopy in living C. elegans was most complicated by large autofluorescence contributions to the measured signals. Heterogeneous absolute and relative fusion protein expression levels were observed, and photobleaching …
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The Role of DNA Binding in Transcription Activation by Estrogen Receptor
… XER. The hormone binding properties and protein expression levels of both the wild-type XER and the XER/2AH mutant are similar. Surprisingly, unliganded XER/2AH exhibited a striking 15-20 fold increase in DNA binding affinity over the wild-type XER. We also obtained the first evidence in …
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Identification of Accessory Proteins and Their Impact on the Expression of the Epithelial Sodium Channel (ENaC)
… Though there have been several accessory proteins known to regulate ENaC function, there are still questions to be answered about the role other proteins play in ENaC trafficking and assembly. Understanding the mechanisms behind ENaC regulation will potentially provide insight into certain …
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Epidermal growth factor-mediated activation of the unliganded glucocorticoid receptor in A549 cells
… epidermal growth factor (EGF) on cytokine gene expression in order to determine whether previously published examples of EGF repressing the expression of interleukin 6 (IL-6) mRNA occur in other cell types and whether the effects of EGF are mediated via the GR. This study provides the first …
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The Tumor Suppressor Notch Inhibits Head and Neck Squamous Cell Carcinoma (Hnscc) Tumor Growth and Progression By Modulating Proto-Oncogenes Axl and Ctnnal1 (Α-Catulin)
… suggested a tumor suppressive role for this protein; however, the mechanism of its function is currently unknown.</p> <p><strong>Procedure:</strong> We used Sanger sequencing and immunoblotting to characterize 50 well- established HNSCC cell lines as being wild-type or mutant for NOTCH1. We …
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Antibody engineering for tumor immunotherapy
… relevant temperatures. In addition, soluble protein expression levels were greatly improved. The final product has a dissociation half-life of approximately 7 days, currently the longest engineered half-life of an scFv against a tumor-associated antigen. Binding and diffusion in …
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The development of a liposomal form Secukinumab – an IL 17 pathway inhibitor in the treatment of psoriasis
… and healthy controls. The regulation of gene expression levels of IL-17, IL-22 and RORC were assessed after treatment with Secukinumab in the bulk and liposome form via RT-PCR analysis. Secukinumab bulk (2.1μg/ml) treatment significantly down-regulated gene expression of IL-17, IL22 and RORC …
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The development of a liposomal form Secukinumab – an IL 17 pathway inhibitor in the treatment of psoriasis
… and healthy controls. The regulation of gene expression levels of IL-17, IL-22 and RORC were assessed after treatment with Secukinumab in the bulk and liposome form via RT-PCR analysis. Secukinumab bulk (2.1μg/ml) treatment significantly down-regulated gene expression of IL-17, IL22 and RORC …
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Molecular mechanisms of radiation-induced brain injury
… transcription factors (e.g., activator protein-1 (AP-1),nuclear factor-κB (NF-κB), and cAMP response element-binding protein (CREB)) and overexpression of pro-inflammatory mediators (e.g., tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and monocyte chemoattractant protein-1 …
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Synthetic biology approaches for engineering bacteria as living therapeutics
… strain design. This platform enables high protein expression levels from the genome of E. coli Nissle through the development of a genetic "landing pad" system and characterization of genetic regulators that can be controlled through the addition of chemical inducers. In my second approach, …
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Quantifying Dyrk1a During Perinatal Development in the Hippocampus, Cerebral Cortex and Cerebellum of the Ts65Dn
The relationship between gene copy number and protein expression levels has not thoroughly been examined in humans or mouse models of Down syndrome (DS) in relationship to developmental changes in the trisomic brain. Found on human chromosome 21 (Hsa21) and triplicated in DS, Dual-specificity …
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Evaluation of PBR in Breast Cancer
… developed to allow non-invasive analysis of protein expression to further characterize tumors. One protein that has been identified as a promising target is the Peripheral Benzodiazepine Receptor: PBR). PBR expression is up-regulated in several cancers, and in many there has been a …
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Heat shock protein 90, a potential biomarker for type I diabetes: mechanisms of release from pancreatic beta cells
Heat shock protein (HSP) 90 is a molecular chaperone that regulates diverse cellular processes by facilitating activities of various protein clients. Recent studies have shown serum levels of the alpha cytoplasmic HSP90 isoform are elevated in newly diagnosed type I diabetic patients, thus …
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Alcohol alters the expression of Soluble N-Ethylmaleimide-Sensitive Factor Attachment Protein Receptors (SNAREs) and spontaneous γ-Aminobutyric Acid (GABA) release...
… investigated the effects of acute alcohol on the expression of genes encoding for synaptic vesicle fusion machinery proteins that form the soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) complex. The proteins in this complex reside on the vesicle membrane …
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Folate: Friend or Foe? An investigation into the opposing roles of folate in glioma
… analysis along with methylation status and protein expression of the genes of interest; PTEN, FOLR1, RFC, PCFT, and MTHFR were analysed to determine differences between cell lines following treatment. The investigation showed that folic and folinic acid behaved differently depending on …
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Investigating the Receptor Interactions and Mechanisms of a Folic Acid-Conjugated Nanoparticle in the Treatment of Breast Cancer
… disrupted. Treatment with FA-DABA-SMA reduces expression of key proteins, including p53, the product of the highly mutated TP53 gene, and additional oncogenic proteins c-Myc, STAT3, HES1, and Notch1, which are all directly or indirectly influenced by FRα. Treatment with FA-DABA-SMA induces …
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