Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"protein export"”.
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Die Funktion von NLP1 im CRM1-abhängigen Protein-Export aus dem Zellkern
Der Export der meisten Proteine aus dem Zellkern ins Cytosol erfolgt unter Beteiligung des Exportfaktors CRM1. Dieser erkennt einen hydrophoben Bereich des zu exportierenden Proteins (NES = nuclear export signal), bindet ihn in Gegenwart von RanGTP und vermittelt anschließend den Durchtritt durch …
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A study of SecA : the motor of the bacterial secretion system by site-directed spin labeling and EPR
… the biochemical details of the Sec system of protein export have been teased out of Escherichia coli. Through these efforts we know that SecA, the ATPase of the Sec system, interacts with several entities: unfolded precursor polypeptides, the molecular chaperone SecB, membrane phospholipids, …
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Understanding the adhesome network in primary and metastatic cutaneous squamous cell carcinoma
… to inhibit invasion and metastasis in patients. Proteins recruited at adhesion complexes, known as the adhesome, are involved in multiple mechanisms which control cancer cell behaviour. Here, we used a proteomic and network analysis approach to perform a global assessment of functional molecular …
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The DNA Transfer Region of Bacteroides Conjugative Transposons Tc(r)Em(r)DOT and Tc(r)ERL
… transposons depends on the regulatory protein RteB, but little is known about how RteB mediates its effect on both elements. This homology may provide a clue about this mechanism of regulation. Mutational analysis of the transfer region has so far revealed genes necessary for …
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Escherichia Coli as a New Platform for the Study of Phosphoinositides
… as integral membrane components, anchors for proteins, membrane identity markers, and signaling molecules. Phosphoinositides are regulated through the phosphorylation and dephosphorylation of the inositol head-group at the 3rd, 4th, and 5th positions, creating a complex and very dynamic …
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The dual-targeted membrane assembly of the <i>Streptomyces coelicolor</i> Rieske protein
There are two major protein translocases for protein export across the bacterial cytoplasmic membrane - the general secretory (Sec) system and the twin-arginine translocation (Tat) system. Both translocases can also insert membrane proteins. The Sec machinery inserts multiple transmembrane domains …
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Trimming Fat Upon Infection: Proteolytic Demyristoylation as a Novel Bacterial Pathogenic Strategy
… pathogens, Shigella injects effector proteins into an infected cell to control its responses. Dedicated type III secretion system delivers more than twenty effectors which highjack normal cell signaling by modulating activity of host proteins. By interfering with immune signaling, …
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Probing the organisation of the TatC component in the Tat system of <i>Escherichia coli</i>
The Tat protein export system transports folded proteins across the bacterial cytoplasmic membrane and the plant thylakoid membrane. In Escherichia coli, the Tat system is composed of the TatA, TatB and TatC proteins. TatB and TatC assemble into a multimeric receptor complex that recognises and …
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Histidine Folding Switch In Effector Protein Avrpto Regulates Its Transport Through The Bacterial Type Iii Secretion System, As Determined By Nmr, Fluorescence, Circular Dichroism Spectroscopies And Translocation Assays
… into host cell cytoplasm for bacterial effector protein trafficking to further their symbiotic or pathogenic goals, while avoiding detection and host immune responses. Gram-negative bacterium Pseudomonas syringae uses the type III secretion system that spans bacterial membranes and extends with a …
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Analysis of essential structural and functional elements in the GTP-regulated signal recognition particle cycle
… signal recognition particle (SRP)-mediated protein targeting using a combination of genetic, biochemical, and molecular sequence analysis techniques. First, I consider the SRP cycle from the perspective of molecular evolution. This analysis provides insight into the significance of …
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Functional metagenomic analysis of carbohydrate degrading enzymes from the human gut microbiota
… the GH9 catalytic domain and identity to the protein from Coprococcus eutactus ART55/1. Further functional analysis established the fibrolytic activity of selected Coprococcus species. Moreover, several active clones were isolated from the Ruminococcus sp. 80/3 genomic library which encoded …
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Defining the Cellular and Molecular Basis of Spondyloepiphyseal dysplasia tarda
… matrix. After folding, newly synthesized proteins reach the Golgi apparatus through COPII carriers. COPII vesicles bud from sub-domains of the ER, named ER exit sites (ERES) and are composed of five rapidly cycling proteins: the small GTPase Sar1, the inner coat (Sec23/Sec24) and the outer …
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Substrate targeting and recognition in the flagellar type III secretion pathway
… flagellar rod, hook and filament requires the export of thousands of structural subunits across the bacterial cell membrane by a specialised flagellar type III secretion system (fT3SS) located at the base of each flagellum. These subunits are unfolded and exported into a narrow 20Å wide channel …
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Characterisation of the structure and function of the Salmonella flagellar export gate protein, FlhB.
… the flagellar rod, hook, and filament requires export of thousands of structural subunits across the cell membrane and this is achieved by a specialised flagellar Type III Secretion System (fT3SS) located at the base of each flagellum. The fT3SS imposes a crude ordering of subunits, with …