Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"protein disulfide isomerase"”.
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Patterns in the sequence context of protein disulfide bonds
Disulfide bonds play an important role in the structural stability of the proteins that contain them. Yet, little is known about the specificity with which they are formed. To address this, a representative set of disulfide bonds from nonhomologous eukaryotic polypeptides was created. The amino …
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Analisi Molecolare del Melanoma
… comparative proteomic analysis revealed that six proteins were overexpressed in both melanoma cell lines as compared with melanocytes: galectin-1, inosine-5'-monophosphate dehydrogenase 2, serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A ¿ isoform, protein DJ-1, cyclophilin A …
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DNA Repair, Redox Regulation and Modulation of Estrogen Receptor Alpha Mediated Transcription
… in turn leads to recruitment of coregulatory proteins. To better understand how estrogen-responsive genes are regulated, our laboratory identified a number of proteins that associate with the DNA-bound ERalpha. Our studies demonstrate that the nonmetastatic protein 23 homolog 1 (NM23-H1) …
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Targeting epithelial senescence to augment renal repair
… in vitro. Inhibition of two of these targets, Protein Disulfide Isomerase Family A Member 3 and Neuropilin-1 attenuate post injury fibrosis in vivo.
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Molecular investigations of gene families encoding foldase enzymes in wheat
The storage proteins of wheat (Triticum aestivum L.) form large polymers commonly referred to as gluten. These polymers are critical in determining important traits, especially in the processing qualities of dough. Vast amounts of research on the storage proteins and the genes encoding them have …
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The degradation of membrane proteins from the mammalian endoplasmic reticulum
Membrane glycoproteins of the secretory pathway that cannot adopt their native conformation are targeted for dislocation from the endoplasmic reticulum (ER) membrane for subsequent degradation by the cytosolic proteasome. This thesis investigates factors influencing the catalyzed destruction of MHC …
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No-Independent Modulation of Soluble Guanylyl Cyclase (Sgc) Activity and Function
… to NO, investigators have identified a number of proteins that interact with sGC and modulate its function. For example, the interaction of sGC with ADP-ribosylation factor GTPase activating protein 1 (AGAP1) governs sGC’s intracellular distribution and therefore mediates localized production of …
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Roles of coagulation factor XIII in the functions of blood platelets
… independently of FXIII transglutaminase and protein disulfide isomerase activities. However, platelet adhesion and spreading were abolished by the Src family inhibitor PP1 indicating a tyrosine kinase-dependent mechanism. Consistent with this, FXIIIa stimulated tyrosine-phosphorylation of …
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Adiporedoxin, an upstream modulator of endoplasmic reticulum oxidative folding and protein secretion
… extracellular matrix, and transmembrane proteins and over expression increased secretion. Together, these results suggest Adrx regulates an early step in protein secretion from the ER. Immunofluorescence and proteolytic protection assays demonstrated that Adrx is located in the ER …
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Characterization of cellular stress systems using biological mass spectrometry
… approach does not only allow assignment of protein identity and identification of sequence specific modifications, but with the help of particular derivatization techniques facilitates the determination of peptide quantity. Each of these approaches were applied to the following biological …
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Translational Frontiers for Chikungunya Virus: New World Epidemic, New Strains, New Therapeutic Targets
… drug targets was explored. As the envelope proteins of alphaviruses are replete with structure-maintaining disulfide bonds, inhibitors to the host enzymes responsible for disulfide bond formation—protein disulfide isomerase (PDI) family chaperones—were utilized as a tool to potentiate PDI as …
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Investigating the molecular biology of aging and extended longevity: Proteomic and genomic analysis of mouse liver
… aging and oxidative damage to ER resident proteins, which exist in a strongly oxidizing environment necessary for disulfide bond formation. In these studies, young and aged mouse liver homogenates were separated into enriched sub-cellular fractions, and the ER/mitochondrial fraction was …
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Analysis and confirmation of the results of a yeast two-hybrid screen carried out to identify proteins that interact with drosophila XRCC2
… DNA damage is brought about by highly specific proteins that partake in a variety of DNA repair. Two of the most common types of damage are double-strand breaks (DSBs) and interstrand crosslinks. A single DSB or crosslink can potentially kill a cell if it is not repaired~ In human and other …
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The role of ERO1 in oxidative protein folding in the endoplasmic reticulum
The formation of native disulfide bonds is critical for the folding and stability of many secreted proteins. We describe an essential S. cerevisiae gene, ER01, which encodes a conserved ER membrane protein required for disulfide bond formation in the er .doplasmic reticulum (ER). In a conditional …
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Utilizing Proteomic Techniques to Discover Host Protein Interactions with the E1 Glycoprotein of Venezuelan Equine Encephalitis Virus (VEEV) for Anti-Viral Discovery
… used to verify V5 insertion within the E1 glycoprotein. Replication kinetics experiments verified the virus replicated similarly to the parental VEEV TC-83 strain, while passaging experiments verified the tag was highly stable for up to 10 passages. This research produced a cost-effective and …
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Investigating neurodegenerative diseases with small molecule modulators
… diseases. Small molecule modulators of protein activity have proven invaluable in the study of protein function and regulation. While inhibitors of protein activity are relatively common, small molecules that can increase protein abundance are quite rare. Small molecule protein …
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Fatty acid synthase is a major polypeptide constituent of cytosolic lipoprotein and is associated with components of the milk lipid secretory pathway
… The major polypeptide constituent of this lipoprotein aggregate was the monomer of fatty acid synthase (FAS). The major milk lipid globule proteins, butyrophilin (8u) and xanthine oxidase (XO), as well as the small GTP-binding protein ARF, also were present. This lipoprotein complex was …
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Disulfide Bond Formation: Identifying Roles of PDI Family Thiol Oxidoreductases and ER Oxidant Pathways
Protein disulfide isomerases (PDIs) catalyze the oxidation and isomerization of disulfide bonds in proteins passing through the endoplasmic reticulum (ER). Although as many as 20 enzymes are classified as PDI family members, their relative contributions to protein folding have remained an open …
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Repulsive cues and signalling cascades of the axon growth cone
… in the literature regarding the need for protein synthesis in the growth cone in response to external guidance cues. My results resolve the conflicting data in the literature on Semaphorin-3A signalling, where differing labs had shown that inhibiting protein synthesis either blocks or has …