Global ETD Search
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Showing 1 to 20 of 274 for “"protein degradation"”.
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Enhanced protein degradation by branched ubiquitin chains
… The anaphase-promoting complex (APC/C) catalyzes degradation of key regulators during the cell cycle causing proper transition through the cell cycle. Here, I report that the APC/C efficiently synthesizes substrate-attached branched conjugates and enhances recognition of ubiquitylated substrates …
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Regulation of Protein Degradation by ADP-Ribosylation
<p>Protein quality control is essential for cellular homeostasis. The Ubiquitin-Proteasome System (UPS) is responsible for the regulated breakdown of intracellular proteins. All proteins are not degraded at the same rate in the cell. For instance, global protein turnover rates in mammals change …
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Studies on Cellular Nutrient Responses and Protein Degradation
… to amino acids. Amino-acid abundance promotes protein synthesis and cell growth via activation of the protein kinase mTOR, while amino-acid deprivation promotes protein degradation by autophagy. The heterodimeric G protein coupled receptor (GPCR) T1R1-T1R3 can act as an extracellular sensor for …
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Regulation of Protein Degradation at the Endoplasmic Reticulum
Endoplasmic reticulum (ER) associated degradation (ERAD) is important for removing damaged or misfolded proteins at the ER membrane, and is central to the physiological regulation of proteins such as HMG-CoA Reductase (HMGCR), the rate limiting enzyme in cholesterol biosynthesis. Under sterol rich …
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Protein degradation from the endoplasmic reticulum in yeast
The majority proteins that misfold in the endoplasmic reticulum (ER) are dislocated to the cytoplasm for degradation by the proteasome. This process of dislocation and degradation occurs in several steps. First, unfolded proteins need to be recognized in the ER. Following recognition, unfolded …
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Functional Characterisation of Protein Degradation during Human Cytomegalovirus Infection
… host immune response, including targeting host proteins for degradation. Insights into this viral antagonism can facilitate the development of novel antiviral therapies by restoring the activity of endogenous proteins important for antiviral restriction and immunity. A previous multiplexed …
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Harnessing the Anaphase-Promoting Complex for Targeted Protein Degradation
Targeted protein degradation (TPD) is rapidly becoming a prevalent modality of therapeutics development due to its event-driven pharmacology that overcomes some major limitations of conventional small molecule inhibitors. One of the paradigms of TPD involves co-opting components of the …
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Pathways of intracellular protein degradation in cultured muscle cells
… for the turnover of endogenous muscle protein, lysosomotropic proteinase inhibitors have been employed to elucidate the relative contributions of lysosomal and non-lysosomal degradation pathways functioning under varying nutritional states and for different classes of intracellular …
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Construction and Biological Characterisation of Custom Engineered Protein Degradation Tools.
… inhibition approaches is Aurora A (AurA). Degradation rather than inhibition of AurA should be able to block non-catalytic functions that cannot be inhibited. Event-driven pharmacology can be effected using bifunctional constructs to recruit the cell’s degradation machinery to target …
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Single-molecule studies of protein degradation and kinesin-8 motility
… and kinesin-8 Kif18B. As members of bigger protein families, these two fascinating nanomachines perform important cellular tasks in protein quality control and spindle formation, respectively. AAA+ ClpX unfoldases use energy from ATP binding and hydrolysis to drive mechanical unfolding and …
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Dynamic Remodeling of The Stressed Heart: Role of Protein Degradation Pathways
… overload activates signaling pathways of both protein synthesis and degradation. While much is known about regulators of protein synthesis, little is known about regulators of protein degradation in hypertrophy. The ubiquitin-proteasome system (UPS) selectively degrades unused and abnormal …
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Targeting Protein Degradation to Uncover Novel Oncoprotein Drivers of Acute Leukemia
… survival of 30–40%. Small molecule induced protein degradation is a novel strategy that can be applied to currently undruggable targets and oncoproteins. In this paradigm, small molecule degraders (either Proteolysis Targeting Chimera (PROTAC) or Molecular Glue (MG)) redirect the cell’s …
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Dual Roles for NBR1 in Bone Remodelling and Autophagosomal Protein Degradation
… of BRCAJJ is a ubiquitously expressed scaffold protein that mediates intracellular functions through protein-protein interactions. The C-terminal UBA domain of Nbrl binds mono- and polyubiquitinated chains whilst the N-terminal PB1 domain interacts with the structurally similar protein p62. The …
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The mechanism of cyclic proteasome-mediated protein degradation in Sulfolobus acidocaldarius
… of the Baum lab showed that proteasome-mediated protein degradation is required for the onset of cell division and at division for the initiation of a new round of DNA replication in the following cell cycle. Division was also associated with the rapid proteasome-mediated degradation of a …
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Regulation of Protein Degradation In The Heart By Amp-Activated Protein Kinase
<p>The degradation of proteins by the ubiquitin proteasome system is essential for cellular homeostasis in the heart. An important regulator of metabolic homeostasis is AMP-activated protein kinase (AMPK). During nutrient deprivation, AMPK is activated and intracellular proteolysis is enhanced …
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Regulation of Protein Degradation In The Heart By Amp-Activated Protein Kinase
<p>The degradation of proteins by the ubiquitin proteasome system is essential for cellular homeostasis in the heart. An important regulator of metabolic homeostasis is AMP-activated protein kinase (AMPK). During nutrient deprivation, AMPK is activated and intracellular proteolysis is enhanced …
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Understanding and harnessing energy-dependent proteolysis for controlled protein degradation in bacteria
Regulated intracellular protein degradation is critical for cellular viability. In many organisms, degradation controls cell-cycle progression, executes responses to stress-inducing environmental changes, and enables the rapid depletion of unwanted or deleterious proteins. In bacteria, most …
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The effect of dissolved oxygen on recombinant protein degradation in Escherichia coli
The production of proteins in recombinant host cells has become an invaluable tool to obtain significant amounts of protein for therapeutic and other applications. Efforts to increase expression of foreign proteins in Escherichia coli, however, put an increasing burden on protein synthesis and …
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Postmortem changes in meat quality and myofibrillar protein degradation in turkey breast muscle
… was assessed using measurements of colour and protein extractability, while quality characteristics of cooked product were assessed using cook yield and torsion gelometry. The extractability and degradation of 10 specific proteins was monitored using SDS-PAGE and Western blotting. Additionally, …
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