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Showing 1 to 13 of 13 for “"protein crosslinking"”.
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Consequences of Oxidative DNA Damage: Base-Pair Dissociation, Singlet Oxygenation and DNA-Protein Crosslinking
… and reaction mechanism involved in DNA-protein crosslinks using the model methylamine (mimics the ε-NH2 group of lysine) reaction with one-electron oxidized 9MG•+ and doubly oxidized [9MG – H]+. It turned out that [9MG – H]+ is more electrophilic and susceptible to crosslinking.</p>
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Molecular mechanisms of the carcinogen chromium(VI)-induced DNA-protein crosslinking in cultured intact human cells
Chromium(VI)-induced DNA-protein crosslinking is implicated in chromium Carcinogenicity. However, the mechanism of chromate-induced DNA-protein crosslinking has not been established. Based on the current literature and our preliminary data, we hypothesized that Cr(VI)-induced DNA-protein crosslinks …
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Use of Protein Crosslinking and Tandem Mass Spectrometry to Study the PsbO, PsbP and PsbQ Extrinsic Proteins of Higher Plant Photosystem II
… and an ensemble of intrinsic and extrinsic proteins which are associated with the photosystem. This metal cluster is stabilized and protected from exogenous reductants by the extrinsic proteins, PsbO, PsbP and PsbQ in higher plants, which are present on the lumenal face of PSII. No crystal …
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Chemical Tools for Exploring IFITM3 S-Palmitoylation and Mechanism
<p>Protein S-palmitoylation is a reversible post-translational lipid modification that regulates the trafficking, stability, and activity of proteins in eukaryotes. The detection of fatty-acylated proteins has been challenging but recent advances in chemical labeling methods have enabled more …
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Tissue Transglutaminase And Its Role In Human Cancer Progression
Tissue transglutaminase (tTG) is a protein-crosslinking enzyme whose expression is up-regulated in several human cancers. Here I show that tTG is a key participant in a novel EGFR/Src-signaling pathway and a potential target for inhibiting EGFR-promoted tumor progression. Specifically, EGF …
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Characterisation of the (H3-H4)2-tetramer and its interaction with histone chaperones
Histones are a major protein component of chromatin, yet mechanisms which control synthesis, post translational modification, deposition and removal of histones are not fully understood. Although atomic resolution structures have been solved for the nucleosome core particle, there is limited …
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Investigation of the role of VML in the establishment of Drosophila embryonic dorsal-ventral polarity
… that transfers sulfate groups to several protein components of the vitelline membrane layer of the eggshell, including Vitelline Membrane-Like protein (VML). These sulfated proteins represent a ventral cue embedded in the eggshell, which, during embryogenesis, leads to the …
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Studies Of The Molecular Mechanisms Underlying Cancer Stem Cells And The Transformed Metabolic Phenotype
… dehydrogenase 1A3 (ALDH1A3) and the GTP-binding protein/crosslinking enzyme tissue transglutaminase (tTG), two proteins suspected to have key roles in tumor initiation and the development of the malignant state, in glioma stem cells (GSCs). Additionally, I examined the contributions of two …
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Site-selective modification of cysteine residues
Proteins are biomolecules that carry out essential work in cells and are required for the structure, function, and regulation of biological systems. It is desired to install designer modifications on proteins of interest, in a site-selective manner mimicking nature's post-translational …
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Foamy Virus Enzymes - Activity, Regulation and Resistance
… Viral particles contain DNA not RNA. The Pol protein, the precursor of the viral enzymes, is translated from a separate mRNA independently of the capsid and matrix proteins. The protease remains covalently bound to the reverse transcriptase, while in orthoretroviruses the protease is cleaved …
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Biochemical Studies of Aromatic Amino Acid Decarboxylases and Acetaldehyde Synthases
… We previously identified two annotated DDC-like proteins from Drosophila indeed catalyzing a decarboxylation-oxidative deamination reaction of L-dopa to form 3,4-dihydroxyphenylacetaldehyde (DHPA), CO2, NH3, and H2O2 and we named these proteins as DHPA synthases due to the physiological …
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Mechanistic study and protein engineering of a dye-decolorizing peroxidase from Thermomonospora curvata for enhanced and expanded functions
… relay pathway, supported by evidence from protein crosslinking, radical trapping, and LC-MS/MS studies. Structural and transient kinetic studies further revealed that this mutation let to the surface radical formation via proton-coupled electron transfer pathways, likely through …
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Biochemical and Rheological Characterization of Peanut Proteins Crosslinked with Microbial Transglutaminase
… used to characterize the soluble and insoluble proteins making up the various dispersions. In the second study, we investigated the polymerization of peanut proteins treated with microbial transglutaminase (TGase). TGase is an enzyme that promotes protein crosslinking reactions through an acyl …