Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"protein corona"”.
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Protein corona formation and analysis on colloidal inorganic nanoparticles
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-08-01
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Impact of human blood plasma-derived protein corona on extracellular vesicle uptake
… EVs have been demonstrated to carry a range of proteins and nucleic acids subsequently delivered to recipient cells, making them attractive as drug delivery vehicles. Several mechanisms for the cellular uptake of EVs have been established. When a nanoparticle is introduced into blood plasma, …
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Development of a click chemistry approach for cancer cell targeting and evaluating the effect of protein corona on active targeting yield
… reaction was also used to study the effect of protein adsorption on active NP targeting. Upon exposure of NPs to the biological environment, proteins and other biomolecules bind to the NPs and cover their surfaces. This protein coating, which is called the protein corona, may reduce the …
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Exploring nanoparticles' interactions with blood proteins and visualizing the heterogeneity within hematopoietic cell populations
… major causes of this unfavorable outcome is that proteins and other biomolecules adsorb onto the particles upon NPs' injection into the blood stream, and form a biological coating named ""protein corona."" Protein corona formation could remarkably affect NPs' blood circulation, their cellular …
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Hard and soft coatings on gold nanoparticles at the nano-bio interface
… of small molecule ligands and biomolecule coronas at the molecular level. Chapter 1 introduces the physical and chemical properties of gold nanoparticles and recent advancement on elucidating the surface chemistry and protein corona formation of gold nanorods. Chapter 2 and Chapter 3 probe …
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Investigating sphingolipid behavior and function using metabolic labeling
… Lastly, in Chapter 5, the cellular uptake of protein-coated nanoparticles was investigated in an effort to understand how plasma proteins interact with the nanoparticle surface, and to enhance the efficiency of targeted nanoparticle delivery with an in vitro system that mimics the in vivo …
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Surface functionalisation techniques for colloidal inorganic nanocrystals
… of aqueous stabilised particles with serum proteins is investigated. In Chapter 3, a new method for the synthesis of a thin silica layer upon the surface of nanocrystals is demonstrated. The method uses the hydrophobic interaction between an amphiphilic polymer and nanocrystal ligands to …
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Chemistry and Measurement at Aqueous Nanoscale Interfaces
… chemistry. The first is an examination of protein corona formation on charged gold nanoparticles with an emphasis on the effect that the deformability of the protein has on the corona formed. The second describes the synthesis of polyamide membrane active layers using a support-free …
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Studies at the nanoparticle-biomolecular interface and beyond
… media was shown to result in a hard and soft protein corona formation, which acted to mitigate/reduce the proposed chemical capabilities of nanoparticles. More critically but even less well understood is the precise orientation of proteins upon adsorption as well as the possible change in …
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Zein-based nanoparticles for drug delivery applications
Zein’s protein origin and hydrophobicity has raised concerns about its potential use as a delivery system in humans. To overcome this issue, it has been hypothesised that conjugating zein with polyethylene glycol (PEG) could provide steric shielding to the delivery system, thus preventing its …
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Drug Delivery to Solid Tumors via Polymeric Nanoparticles
… is hypothesized to be premature drug release or protein corona formation.
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Physical-chemical characterization of nanoparticles in relevant biological environments and their interactions with the cell surface
… with the biological matter and formation of a protein corona (PC) coating the NP. The PC determines the NP biological identity and it is the ultimate interface with the surrounding environment. Therefore, a deep characterization of the NPs in biological media is important to predict adverse …
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The Impact of Lipid Nanoparticle Chemistry on RNA Delivery and Therapeutic Outcomes
… able to functionally deliver RNA to hepatocytes. Protein corona analysis indicated that 5A2-SC8 LNPs bind Apolipoprotein E (ApoE), which can drive LDL-R receptor mediated endocytosis in hepatocytes. In contrast, the surface of 3A5-SC14 LNPs was enriched in Albumin but depleted in ApoE, which …
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DESIGNING BIOMIMETIC NANOPARTICLES TO TACKLE INTRACELLULAR PATHOGENS
… thickness of approximately 6.3 ± 1.2 nm. Minimal protein corona formation was detected using fluorescence correlation spectroscopy (FCS), suggesting desired biomimetic characteristics. Lectin staining confirmed plasma membrane origin and preservation of surface glycocalyx profiles. Partial …
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Molecular engineering of gold nanorod surfaces: towards improved physical properties and understanding nanoparticle-cell interactions
… nanorods were observed due to spontaneous protein adsorption. In growth media, all examined nanorods were covered with protein corona and thus bear similar negative effective surface charge explaining their similar toxicity profiles. ! """"""! ! Our covalent approach to stabilize the …
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Next generation multi-color carbon dots: A comprehensive understanding of their photophysical properties and subsequent use for biomedical applications
… patterns in presence of human digestive enzymes, proteins: examining the protein corona formation with varying surface chemistry using an on-chip electrical monitoring setup, and oligonucleotides: utilizing them to deliver intracellular cargo and monitoring of biological events. Overall, I believe …
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Single-Molecule and Ensemble Measurements Inform on the Role of Protein Crowding at Interfaces
Surface-adsorbed proteins can critically affect the efficacy of materials designed for biological applications. For example, physiologically relevant quantities of serum albumin stabilize nanoparticles in solution, while decreased protein concentrations induce nanoparticle aggregation. The first …
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Nanoparticle toxicity and molecular mechanisms in fish: A case study with silver nanoparticles
… in this area. In Chapter 2, we show that blood proteins can quickly bind to the surface of NPs, which might greatly change their biological identity. We present novel data on the formation of a “protein corona” (PC) after incubation of polyvinylpyrrolidone-coated AgNPs (PVP-AgNPs, 50 nm) in fish …
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Development of Dendritic Polymers as a Modular Drug Delivery Platform for Avascular Tissues
… of this thesis improved the bioconjugation of proteins to PEG-PAMAM conjugates and expanded the platform to other biologics and tissues. A robust scheme using click chemistry was introduced, improving loading efficiency while conserving protein bioactivity. Protein content influenced drug …
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