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Showing 1 to 20 of 52 for “"protein conformation"”.
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Protein conformation from electron spin relaxation data
Submitted by Carolyn Mead (cmead2@illinois.edu) on 2011-06-13T17:50:56Z No. of bitstreams: 1 1982_allen.pdf: 2854645 bytes, checksum: 6b938b205e05f714ee2780d1ad6b4369 (MD5)
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Molecular Dynamics Studies of Interfacial Effects on Protein Conformation
… several systems in which interfacial effects on protein conformation are of interest. In the first chapter, a detailed description of MD simulations is presented. The physical basis for the methodology is discussed, followed by a description of the algorithms implemented to carry out the …
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Direct real-time correlation of protein conformation and substrate recognition
Conformational transitions in a protein and its interaction with the cognate substrate exemplify two important biomolecular processes that may be correlated, uncorrelated, or partially correlated. While the degree to which these processes are correlated may bear heavily on the mechanism and …
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Structural and Dynamic Aspects of the Cytochrome P450(cam) Protein Conformation
… examine the relationship between this internal protein equilibrium and important functional aspects of the cytochrome.
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Computing free energy hypersurfaces for anisotropic intermolecular associations
… used for calculating the free energy of conformation and chemical reactions amongst others. Here a generalized method is described that is able to produce free energies in multiple dimension, descriptively named the free energies from adaptive reaction coordinate forces (FEARCF) method. …
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Oxidation of methionine residues in protein pharmaceuticals
… developed. It allows an accurate prediction of protein oxidation, and provides a rationale for developing strategies to control oxidation, such as modulating protein conformation via adding excipients. This knowledge could aid in developing in a more rational manner solvent formulations that …
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Investigating Ligand Induced Conformational Changes in the Estrogen Receptor
Primarily, we have investigated ER conformational structure using the reporter group method of site-directed spin labeling (SDSL), along with electron paramagnetic resonance (EPR) spectroscopy. In this work, B and C parameter calculations from EPR line-shape theory express receptor differences …
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Imaging of mice and men; adventures in multispectral imaging
… the neurodegeneration. Prions are a mis-folded protein which have a different conformation than the normal protein. Certain spectral features in the mid infrared region are associated with protein conformation. In collaboration with neuro-biologists within the university and using a synchrotron …
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Development and Applications of Liquid Sample Desorption Electrospray Ionization Mass Spectrometry (DESI-MS)
… sample DESI are discussed in details including protein conformation study, fast reaction kinetics study, and the coupling with LC for enhancing protein signal and charge. In liquid sample DESI, direct ionization of continuous-flow liquid samples was achieved without the sample drying. In this …
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Prion biology in the context of bacteria
… been uncovered in yeast, where they function as protein-based units of heredity that confer unique phenotypic traits on those cells that harbor them. To date, the discovery of prions and prion-like phenomena has been confined to the Eukarya. The work presented in this thesis seeks to explore the …
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Solid-state NMR investigations of the bacteriorhodopsin photocycle
… (bR) is a heptahelical integral membrane protein with a retinylidene prosthetic group from H. salinarum. The protein creates a proton gradient across the cell membrane by harvesting light energy. Proton transfer events can be segregated into transfers between the Schiff base of the …
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Predicting protein residue-residue contacts and disorder
… OF MISSOURI AT AUTHOR'S REQUEST.] Predicting a protein's three dimensional structure from its corresponding sequence has long been an extremely important and challenging problem in the field of Structural Bioinformatics. A principle difficulty has been in efficiently exploring the large number …
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Preparation of 7-(p-Substituted-Benzylamino)-4-Nitrobenzoxadiazole Derivatives and the Study of their Fluorescent Properties in Solution
… others as a fluorescent probe to determine the conformation changes of bovine trypsinogen and bacterial libosomes. Fluorescent probes have been used to: (l) establish the degree of polarity of a particular region of a protein; (2) measure distances between groups in a protein; (3) determine the …
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Domain swapping as a molecular mechanism in amyloidosis
… a group of diseases characterized by a change in protein conformation resulting in aggregation and tissue deposition of amyloid fibrils. One variant of cystatin C, L68Q cystatin C, is highly amyloidogenic and persons carrying the corresponding gene suffer from massive cerebral amyloidosis leading …
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Protein design using artificial intelligence
… the examination of the fundamental problem of protein folding, focusing on AlphaFold and its unprecedented accuracy in predicting protein structures, which rivals experimental methods. The chapter discusses both the innovations brought forth by these models and their technical limitations. …
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Investigating the mechanotransduction by two-photon fluorescence microscopy measurement of intracellular free energy of binding
… binding constant between focal adhesion proteins of interest, GFP-Paxillin and FAT-mCherry, using two-photon excitation fluorescence microscopy and the utility of it as a measure of protein conformational change. The hypothesis tested is that force-induced changes in protein conformation …
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The regulation of yeast homotypic vacuole fusion by phosphatidic acid and diacylglycerol
… and lumenal content mixing. The roles of proteins that carry out membrane fusion stages are well established, however the roles regulatory lipids at the membrane play are still unclear. Many specific regulatory lipids localize to the site of fusing vacuoles termed the “vertex ring” …
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Improving Biocompatibility By Controlling Protein Adsorption: Modification And Design Of Biomaterials Using Poly(Ethylene Glycol) Microgels And Microspheres
… responses are initially elicited and directed by proteins that adsorb from this multicomponent solution to form thin films on their surfaces. The identity, conformation, and quantity of adsorbed proteins are related to the properties of a material's surface. For example, hydrophobic surfaces tend …
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The effects of extended illumination on CO rebinding to myoglobin
… below 160K where CO is trapped within the protein after photolysis. The process increasing the rebinding barriers is found to be a photon-induced rather than a thermal effect. Rebinding barriers of molecules that are photolyzed do not increase unless light is incident on the sample. The …
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