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Showing 1 to 20 of 305 for “"protein complex."”.
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Quality control of protein complex assembly
All cells contain numerous multi-subunit protein complexes of exceptional abundance and physiological importance. Prominent examples include the ribosome, proteasome, chaperonin, tubulin, haemoglobin, and many others. A major challenge for the cell is to synthesise individual subunits in the …
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High-Throughput Determination of Mycobacterium smegmatis Protein Complex Structures
… incorporating the role of stable and transient protein-protein interactions which govern cell function and behaviour. Although there are many in vivo and in vitro methods for studying protein-protein interactions, they suffer from the lack of ability to distinguish physiological interactions …
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Aneuploidy reveals insights into control of protein complex stoichiometry
… physiology and places intense demand on the protein quality control system of the cell to effectively fold and/or degrade proteins. Aneuploidy further represents an ideal model for studying how cells cope with imbalances in their proteome as it allows for interrogation of the fate of hundreds …
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Assembly and biochemical properties of a human chaperone/co-chaperone protein complex
The 70kDa members of the heat shock protein family (eg. Hsp70) function as molecular chaperones by binding to exposed hydrophobic patches on nascent polypeptides forming non-covalent interactions, thereby preventing their aggregation and facilitating their proper folding. The folding reaction …
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An investigation of the function of adaptor protein complex 4 (AP-4)
… organelles. During vesicle trafficking, proteins are selectively sorted into membrane bound transport intermediates by vesicle adaptors, which include those of the highly conserved adaptor protein (AP) complex family. Each AP complex has a distinct subcellular localisation and functions …
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The Role of GRA7, a Component in the ROP18 Protein Complex, in Toxoplasma gondii
… rhoptry (ROP) and dense granule (GRA) family of proteins. On the PVM, ROP18 kinase targets the host's dynamin-related immunity related GTPases (IRG) for phosphorylation on critical residues, leading to their inactivation. Avirulent strains that lack ROP18 are successfully targeted by the IRG …
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Purification and Characterization of a Methyl-DNA Binding Protein Complex from Primary Erythroid Cells
… in part by DNA methylation.A methyl-DNA binding complex, termed the MeCPC (Erythroid Methyl Cytosine-binding Protein Complex), has been found to bind to the methylated, but not unmethylated, ρ-globin promoter and proximal transcribed region in nuclear extracts from definitive erythrocytes. This …
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Characteristics of the Recovery of the Coenzyme A-Synthesizing Protein Complex from Saccharomyces Cerevisiae
<p> A multienzyme complex contained in Bakers' yeast (Saccharomyces cerevisiae) which synthesizes CoA has been named the coenzyme A-synthesizing protein complex (CoA-SPC). The CoA-SPC has been shown to be insoluble in the crude Bakers' yeast cell lysate formed by exposing the yeast cell to ether …
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The effect of macromolecular crowding on the structure of the protein complex superoxide dismutase
… of macromolecular crowding on the size of a protein complex, superoxide dismutase (SOD). Crowding was induced using 400 MW polyethylene glycol (PEG), triethylene glycol (TEG), methyl-alpha-glucoside (alpha-MG) and trimethylamine N-oxide (TMAO). Parallel small angle neutron scattering (SANS) …
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Development of Ion Mobility Mass Spectrometry Instrumentation to Investigate the Gas-Phase Structures of Protein and Protein Complex Ions
… spectrometry (IM-MS) instrumentation to analyze protein and protein complex ions. IM-MS is a gas-phase analytical technique that separate ions based on their collision cross section (a description of ion shape) and mass-to-charge ratio. Electrospray ionization of samples from buffered solutions …
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Characterisation of Small Ubiquitin-like Modifier (SUMO) protein complex expression in human cancer cells to identify novel therapeutic targets
… where a number of small ubiquitin-like modifier proteins (SUMO) are conjugated to lysine residues of the target proteins and contribute to various cellular functions essential for cancer cell survival and proliferation. The unique behaviours, phenotypic and molecular adaptations found in the …
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Computational Investigations of Cellular Functions: Three Cases on Membrane Morphogenesis, Organization and Assembly of a Multi-Protein Complex, and the Molecular Origin of Muscle Elasticity
Titin is a mechanical protein that protects muscle from overstretching by producing a restoring force when a muscle fiber is extended beyond its normal length. Force spectroscopy studies have shown that titin exhibits several regimes of elasticity. Disordered segments bring about a soft, entropic …
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Computational investigations of cellular functions: Three cases on membrane morphogenesis, organization and assembly of a multi-protein complex, and the molecular origin of muscle elasticity
… of the biological membrane, assembly of a multi-protein complex, and the molecular origin of elasticity in muscle protein. In the photosynthetic purple bacterium Rhodobacter (Rba.) sphaeroides, light is absorbed by membrane-bound light-harvesting proteins LH1 and LH2. LH1 directly surrounds the …
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Isolation of a carotenoid-protein complex from carrot root and the effects of components of carrot and heat on the relative bioavailability of carotenoids from carrots
… work are: (1) to isolate and purify carotenoid-protein complexes from carrot roots and to determine the location of carotenoid-protein complexes within the carrot chromoplast, and (2) to utilize the ferret to evaluate the effects of carotenoid-protein complexes, crystalline form of carotenoids, …
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Characterization of the interaction of putrescine and the adenosine-3' ,5'-cyclic monophosphate-cAMP receptor protein complex in the regulation of the speC gene encoding ornithine decarboxylase in Escherichia coli
… by either putrescine or the cAMP- cAMP receptor protein (CRP) complex. To determine whether these regulatory modes are independent, the expression of ODC was determined by measuring the specific activity of ODC in crude extracts prepared from exponentially grown cultures of wild type ~ coli K-12 …
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Regulation of de-etiolation and stomatal opening responses by HYPOSENSITIVE TO LIGHT and HYPERSENSITIVE TO RED AND BLUE 1.
… defects. HTL belongs to an alpha/beta fold protein family and is localized predominantly in the nucleus. ELONGATED HYPOCOTYL5 (HY5), a BZip transcription factor, directly binds to both C/G-box and G-box in HTL promoter and regulates HTL expression. Evidences suggest that HTL represents a new …
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Biological Networks: Modeling and Structural Analysis
… models for representing experimental data on protein interaction networks and protein complex networks (protein complexes are groups of proteins that associate to accomplish some function in the cell), and to design algorithms for exploring such networks. Our goal is to enable biologists to …
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Electron Transfer Reactions of the Ubiquinol: Cytochrome C-2 Oxidoreductase of Rhodopseudomonas Sphaeroides
… c(,2) oxidoreductase (b-c(,1) complex) of the photosynthetic bacteria Rps. sphaeroides have been investigated through the use of optical spectroscopy and chemical inhibitors of the protein complex.
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Identification and Characterization of Pat-6/actopaxin in Caenorhabditis Elegans Body Wall Muscle
In addition, I identified PAT-4/ILK and a novel protein, K10B2.5, as putative binding partners of PAT-6/actopaxin. PAT-6/actopaxin and PAT-4/ILK colocalize in vivo, forming a multi-protein complex with UNC-112. The accumulation of PAT-6/actopaxin in body wall muscle cells requires PAT-4/ILK kinase …
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