Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"protein clustering"”.
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Spatiotemporal Control of Actin Cytoskeletal Machinery in Cells: Multivalency Mediated Protein Clustering at the Plasma Membrane and Optogenetic Tool Development
… membrane is organized into distinct lipid and protein microdomains. Recent work has shown the importance of protein assembly at the membrane in the formation of these domains. However, the mechanisms by which proteins organize at the membrane are largely unknown. Here I demonstrate that …
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Studies on Combining Sequence and Structure for Protein Classification
… is to develop a better understanding of how proteins evolve different structures and functions. A large scale protein clustering can provide a useful platform to identify such principles of protein evolution. Manual classification schemes accurately group homologous proteins, but they are …
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Protein structure and interaction under environmental stress : from quality control recognition to evolution of collective behavior
A protein's function in the cell depends on its structure, which in turn depends on the intracellular environment. Stress like heat shock or nutrient starvation can alter intracellular conditions, leading to protein misfolding - i.e. the inability of a protein to reach or maintain its native …
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GPCR to Channel Communication in a Membrane Signaling Pathway
<p>G-protein-coupled receptors (GPCRs) regulate a variety of downstream effector proteins, making them essential for many physiological processes. For example, in the heart, the activation of beta-adrenergic receptors (βARs) and the stimulatory G protein (Gαs) leads to the opening of downstream …
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Characterization of CEACAM1 Spatial Distribution, Self-association and Dynamics using High-resolution Microscopy Techniques
CEACAMs are cell surface glycoproteins involved in homo- and heterophilic intercellular interactions that control cellular growth, differentiation, tumourigenesis, inflammation and infection. There is growing evidence that CEACAM1 isoforms regulate the cellular response to CEACAM1-mediated …
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Dynamics and Turnover of Lipid and Protein Domains in Cells and Supported Bilayers
<p>Protein and lipid clustering is an important mechanism for cell processes such as exo- and endo-cytosis and creating functional signaling complexes. It has been seen that both lipids and proteins are involved with the formation of cluster domains on cell membranes, yet little is known about the …
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Theoretical and lab based studies of fluorescence anisotropy toward the analysis of multiple homo-FRET pairs
Many biological functions, involve assembly of proteins. Protein clustering has an important role in signal transduction through the cell membrane. Model systems that simulate receptor aggregation can be used to monitor oligomerization. Techniques based on homo-FRET can reveal the aggregation state …
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Immunoproteome of anti-TB drug associated DRESS in HIV TB co-infection
… We hypothesize that differentially regulated proteins would be found interindividually and with disease evolution that may provide a mechanistic understanding of DRESS underlying pathways. Participants and Methods: HIV cases with DRESS (probable or definite) and confirmed reactions to either …
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Toca-1 driven actin polymerisation at membranes
… for the formation of these protrusions. The proteins that are thought to fulfil such a role have a membrane interacting domain (such as the PH domain in lamellipodin, or I-BAR protein in IRSp53) and a domain which interacts with actin regulatory proteins (such as the SH3 domain of IRSp53, …
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Quantitative super-resolution imaging of cell polarity proteins using DNA-PAINT
… the localisation and spatial organisation of proteins is crucial for understanding their function. The development of super-resolution imaging has improved our ability to garner this information, but counting individual molecules in densely-packed assemblies is still challenging. DNA-based …