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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"protein chemistry"”.
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Oxygen toxicity and radiation injury to the pulmonary system / by Geoffrey McLennan.
… include cell culture with function studies, protein chemistry, animal and human physiology, and cell and lung structure through histopathology, and various forms of electron microscopy. The work resulting from this thesis has formed an important basis for understanding acute and chronic lung …
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A Selection for Intein Splicing inPhage Assisted Continuous Evolution
Developing tools for target manipulation of protein chemistry remains a longstanding goal in chemical biology. Trans-splicing inteins fulfill a crucial niche in this area by enabling post translational splicing of separate polypeptides, introduction of post translational modifications, and other …
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Complement evasion strategies of human pathogens - the evolutionary arms race
… I, and its two major cofactors C4b-binding protein (C4BP) and Factor H. This mechanism contributes to complement resistance of this bacterium. We also found that major periopathogens have the ability to bind to the membrane-bound complement inhibitor CD46. Strikingly, even though this …
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Calcification by amorphous carbonate precursors: Towards a new paradigm for sedimentary and skeletal mineralization
… under biological control through regulation of protein chemistry and the local mineralization environment. A review of how foraminifera produce their test structures is also examined to explore the question of how organisms regulate the Mg content in proportion to the temperature their …
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The mechanism of an unusual benzo/benzo photocycloaddition reaction : photophysical and photochemical investigation of [3.3] orthocyclophanes with two face-to-face preoriented benzenoid chromophores
… become a topic of great interest in structural protein chemistry. For three structurally closely related [3.3] OCPs the photophysical - absorption, transient absorption; fluorescence emission, excitation, lifetimes, quantum yields; phosphorescence - and photochemical properties - photoreaction …
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Towards targeted post-translational modification of endogenous proteins in complex environments
Proteins are commonly thought of as polymers comprising the twenty natural amino acids. However, in actuality, the proteome of a typical cell contains hundreds of different amino acid side chains, brought about by co- and post-translational modifications (PTMs). Depending on the roles of the …
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Wechselwirkung von dünnen Schichten aus HVZ Polyestern im wässrigen Medium mit Modellproteinen
… sensitive techniques with respect to further protein adsorption investigations. In Chapter 3.3 the strategy for the fabrication of surface attached carboxyl terminated HBP using "grafting to" technique is developed. The Chapter 4 consists of two parts: The first (theoretic) part …
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Membrane trafficking of ATP-sensitive potassium channels
… channel synthesis as shown by both immunocytochemistry and protein chemistry in both INS-le and isolated mouse pancreatic ß-cells. The intensity of fluorescence associated with Kir6.2 and SUR1 was - 2.5 fold greater in cells incubated with 3 mM compared to 25 mM glucose. This sudden increase …
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A Critical Evaluation of Screening versus Sample-by-Sample Analysis of Protein Aggregation using a Variety of Chromatographic and Spectroscopic Techniques
While protein aggregation has been widely studied, it is still poorly understood. Protein aggregation is a complex process, resulting in heterogeneous populations of particles from a variety of mechanisms, which provides both intellectual and technical challenges. This thesis probes the mechanisms …
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Importance of zein composition in quality protein maize (QPM) to microstructural and chemical modifications of proteins during extrusion and their impact on texture of corn-based extrudates
… out to clarify the importance of endosperm corn proteins in corn grain hardness and vitreousness, the role that protein compositional differences plays on the physical properties of texturized corn-based products is still poorly understood. Considering that protein has been shown to have an …
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Molecular investigation if the rat jejunal active nucleoside transport protein rCNT1
… and the tissue distributions of these proteins in detail, and thus to elucidate their physiological roles. The first of these transporters to be cloned was rCNT1, a protein from rat jejunal epithelium which exhibits pyrimidine-selective, cit-type transport activity. The present study …
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Characterization and evolution of artificial RNA ligases
… and research. Great progress has been made in protein engineering to modify properties such as stability and catalytic activity of an enzyme to suit specific processes. On the contrary, the generation of artificial enzymes de novo is still challenging, and only few examples have been reported. …
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The impact of genetic variation on functional, technological and digestive properties of β-lactoglobulin
Genetic variations of milk proteins have been studied since their discovery in 1946. Since then, over 50 variants have been found among the four caseins (αs1-, αs2-, β-, and κ-casein) and the two main whey proteins, β-lactoglobulin (β-lg) and α-lactalbumin. In the early 1990’s, it was speculated …
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Exploring induced secondary structure and unmethylated DNA binding domains of methyl CpG binding protein 2 (MeCP2)
Our understanding of Methyl CpG binding protein 2 (MeCP2) structure and function has changed and expanded considerably over the last two decades. Mutations along the entirety of the human MeCP2 gene product lead to a disease state - Rett syndrome. The clinical connection of this protein has …
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Development and application of OmpT-based middle down proteomics
"Information at the protein level is essential to understand the functioning of a biological system. The goal of proteomics is to provide such information as protein identities, post-translational modifications (PTMs) on proteins, and ultimately, quantification of proteins or their PTM levels from …
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Targeting isoprenoid and quinone biosynthesis for antimicrobial discovery
"Enzymes in isoprenoid biosynthesis pathway play important roles in all living organisms. Here we report the first structure of heptaprenyl diphosphate synthase from Staphylococcus aureus (SaHepPPS) together with an investigation of its mechanism of action, and inhibition. HepPPS is involved in …
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Observing the effects of acetylcholinesterase and brain-derived neurotrophic factor on the levels of soluble amyloid precusor protein α in lung cancer cell media
… to this the levels of soluble amyloid precursor protein α (sAPPα) were higher in H1299 cell media compared to A549 cell media. With these previous findings, we hypothesize that the levels of sAPPα are regulated by AChE and mBDNF. When cells were treated with AChE, sAPPα levels were decreased …
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Papain-papaya protein interactions : resistance of papaya protein to proteolytic digestion by papain
… action on both natural substrates (native papaya protein and bovine serum albumin) and artificial synthetic substrate (benzoyl~l-arginine ethyl ester). Following removal of 30 percent and 40 percent of the amino acid residues from the amine terminus, the activity of the degraded papain was …
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Molecular Level Interaction of Human Fibroblast Growth Factor-1 (hFGF-1) With Phloridzin
… of growth factors which includes twenty three proteins. FGFs work as modulators for various cellular activities like mitosis, differentiation and survival. Among the FGF family, human fibroblast growth factor-1 (hFGF-1), which is also known as acidic fibroblast growth factor, is a potent …