Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"protein characterization"”.
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Protein Characterization by Mass Spectrometry
… opportunity to develop methods that facilitate protein characterization. This thesis utilizes matrix-assisted laser desorption/ionization and electrospray ionization MS to explore protein structure. Starting at the 'primary' level of protein structure, an approach is developed to aid in the …
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Applications of mass spectrometry to protein characterization
The application of mass spectrometry as a protein sequencing method is demonstrated by the study of bovine kidney aldose reductase. The protein is blocked at the N-terminus and is not amenable to Edman degradation. Using a strategy of homology comparison, the amino acid sequence was determined …
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Nanopore systems for protein characterization and biomimicry
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2026-12-01
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Protein characterization using a computational microscope: Nanopore sequencing and biological condensates
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-08-01
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Reducing Dietary Crude Protein: Characterization of Branched-Chain Amino Acid Antagonism in Modern Broiler Chickens
<p>Reducing the crude protein content of broiler diets while maintaining performance has been a primary research area in nutrition. During the late 20th century, the bulk of this research has involved supplementing broiler diets with feed-grade amino acids and proving the economic benefits of their …
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Multiplexed immunoassay development for precision medicine diagnostics and protein characterization using silicon photonic microring resonators
… therapeutic antibodies as a means of structural characterization. Finally, Chapter 6 discusses the future of the LTBI diagnostic signature development, outlining a significant expansion of the biomarker panel, informatic analysis, and subject study enrollment.
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The Development of Genetic Tools for Protein Characterization and Genetic Engineering in the Acetic Acid Bacterium Gluconobacter Oxydans
… of tools have been constructed: a fluorescent-protein based screening system, a surface display system, and an inducible promoter system. The results of the construction of these three tools for use in G. oxydans and initial analyses of the effectiveness within the host are presented.
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Bioinformatics of High Throughput Proteomics Using Tandem Mass Spectrometry of Intact Proteins
… to the problem of identifying and characterizing proteins with a DNA-predicted sequence. It would be desirable to move top down mass spectrometry in to the 'omic' sciences by developing a high throughput form that could be used to simultaneously identify and characterize thousands of proteins. …
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The Termite Digestome: Understanding the Digestive Physiology Involved in Lignocellulosic Biomass Degradation
… degradation of lignocellulosic biomass. Various protein characterization and gene expression methods were used throughout this research in order to accomplish these objectives. The results of this dissertation indicate that: 1) termites and their symbionts act in a synergistic manner to degrade …
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Characterization of Mycobacterium avium cytoplasmic membrane proteins with an emphasis on the major cytoplasmic membrane protein
Proteins of the cytoplasmic membrane of Mycobacterium avium were investigated to identify those which were: (1)intrinsic or extrinsic, (2) attached to the cell wall, (3)surface accessible and (4) excreted. In addition sera containing anti-cytoplasmic membrane proteins were obtained and preliminary …
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Approaching the Complexity of Biological Specimen: New Mass Spectrometry Concepts in Ambient Ionization and Proteomics
… and depth profiling of limes. Lime layer characterization is performed using data-dependent acquisition, identifying important compounds such as malic acid, citric acid, and quinic acid. Depth profiling is performed by constructing an automated syringe pump system, where the …
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Characterization of novel prophages in lysogenic murine isolate lactobacillus murinus EF-1
… into a host arginine t-RNA. Additional putative protein characterization of phiEF-1.1 revealed coding regions to be divergent from 104 viral genomic coding regions through BLASTp amino acid sequence percent similarity. The low amino acid sequence identity of two murinus L. murinus GIT isolates …
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Computational Methods for Accelerated Discovery and Characterization of Genes in Emerging Model Organisms
… for this alga. Next, we develop a new automated protein characterization method that facilitates the transfer of information across different protein families by extending simple homology categorization to identify new cilia gene candidates. Finally we perform and analyze high-throughput …
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Characterization of signaling pathways underlying key growth and development processes in Populus trichocarpa
… or of various wood properties. This led to the characterization of 165 protein-protein interactions and 20 protein-DNA interaction which constitute numerous woody tissue related subnetworks. One such network, centered on the DIVARACATA and RADIALIS INTERACTING FACTOR (PtrDRIF), identified …
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Multi-target Prediction Methods for Bioinformatics: Approaches for Protein Function Prediction and Candidate Discovery for Gene Regulatory Network Expansion
… of their workflow. Given the central role of proteins in living organisms, in this thesis we focus on their functional analysis and the intrinsic multi-target nature of this task. To this end, we propose different predictive methods, specifically developed to exploit side knowledge among …
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Improving the Performance and Precision of Bioinformatics Algorithms
… for analyzing both genomic (DNA) and proteomic (protein) data with high speed and accuracy have thus become very important in modern biological research. This thesis presents several techniques for improving the performance and precision of bioinformatics algorithms used by biologists. …
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Identification and characterization of a novel structural protein of porcine reproductive and respiratory syndrome virus, the replicase nonstructural protein 2
… economic consequence. The PRRSV non-structural protein 2 (nsp2) is a large multifunctional protein encoded by the most genetically diverse region of the genome - the selective pressure potentiating mutation within this region is unknown. Here we report the identification of a unique function of …
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Development of ion mobility mass spectrometry deconvolution strategies for use in data-independent acquisition proteomics.
… suitable analytical tools for high-throughput protein characterization. However, limitations in ion mobility (IM) and mass spectrometry (MS) resolving powers can limit the success of MS-based proteomics approaches. This work highlights the development of IM-MS deconvolution techniques and their …
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