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Showing 1 to 20 of 25 for “"protein biochemistry"”.
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Exploring and Exploiting Ribonuclease 1: from Protein Biochemistry to Protein Engineering
Ribonuclease (RNase) 1 is a human protein with a remarkable ability to indiscriminately hydrolyze RNA. RNase 1 and its bovine homologue RNase A exhibit ubiquitous expression across tissues, a catalytic efficiency within the diffusion-limited regime, and minimal substrate sequence requirements. …
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Characterizing CMT-causing variants in tryptophanyl- tRNA synthetase
… synthetase (WARS1). Using proteomic analysis, protein biochemistry, and yeast growth assays, I found that while both variants retain their typical structure, their stability and function differ. D314G exhibited decreased stability and triggers stress- related pathways, whereas H257R showed mild …
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Biocompatibility and Bioactivity of Calcium Silicate-based Dental Bioceramics
… viability assays<br/>3)Immunocytochemistry<br/>4)Protein Biochemistry<br/>5)mRNA expression analysis<br/><br/>Visual observations demonstrated differing changes in cellular morphology between our two main test materials and cell lines, with gingival fibroblasts demonstrating a more spindle like …
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Overcoming barriers in chemistry : a birds eye view of electrostatic fluctuations in fundamental proteins and streamlining inclusivity in the classroom.
… interactions play an important role in protein biochemistry. The net charge of a protein (Z) can influence the structure, function, and solubility but is often overlooked in classical biochemistry. In Chapter two, I investigate the ability of a Rieske Iron-Sulfur (ISP) metalloprotein to …
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Engineered metalloproteins as contrast sensors for molecular fMRI
… genetically encoded sensors based on fluorescent proteins have provided an engineerable platform on which to optimize desirable device characteristics by exploiting the tools of molecular biology and protein biochemistry. Analogously, we seek to build genetically encodable sensors based on …
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Biochemical and spectroscopic insights into peculiar active site structures and biosynthesis of O2-tolerant [NiFe]-hydrogenases
… of a NiFe(CN)2(CO) moiety coordinated to the protein by four strictly conserved cysteine residues. Two of them serve as terminal ligands to the Ni and two are bridging ligands coordinating both, the Ni and Fe. The Fe is additionally equipped with one carbon monoxide (CO) and two cyanide (CN–) …
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A NOVEL, RAS-INDEPENDENT ROLE FOR NF1 IN MICROTUBULE DYNAMICS AND DAMAGE REPAIR DICTATES SENSITIVITY TO T-DM1 IN HER2 POSITIVE BREAST CANCER.
… activity of NF1, its GTPase-activating protein (GAP) function that attenuates RAS signaling. NF1 encodes Neurofibromin (NF1), a large multifunctional protein consisting of 2,818 amino acids. Because of its large size and difficulty in cloning, NF1 structure has resisted characterization …
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Design and development of novel antagonists targeting Cdc20, a central regulator of the Anaphase-Promoting Complex/Cyclosome, for the treatment of cancer
… 20 (Cdc20), a central SAC regulator and mitotic protein, binds the Anaphase-Promoting Complex/Cyclosome (APC/C), a complex capable of stipulating protein destruction, regulating its activity and driving cell cycle progression through orderly APC/C substrate degradation. Key oncogenic roles and …
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Investigation of asparagine-linked glycosylation in archaeal and bacterial systems
Asparagine-linked protein glycosylation entails the stepwise assembly of an oligosaccharide onto a polyisoprenyl diphosphate carrier, followed by the en bloc transfer of the glycan onto acceptor proteins by oligosaccharyl transferase (OTase). It is now clear that this N-linked protein modification …
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Mechanisms of Adsorption and Surface-Mediated Aggregation of Intrinsically Disordered Protein Tau at Model Surfaces
… of an intrinsically disordered soluble protein, tau, into insoluble filaments is a defining hallmark of many neurodegenerative diseases, commonly referred to as tauopathies. In its native state, the protein tau’s function is to promote the assembly, and aid in the stabilization of …
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Modification of Biopolymers Using Palladium Oxidative Addition Complexes
… via a brief historical account of the field of protein biochemistry. Other details such as the therapeutic role of key biopolymers, chemical modification strategies, palladium organometallic chemistry, and the recent developments in Pd-mediated bioconjugation are provided as well to give context …
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Biochemical and Functional Investigations into the Contribution of SOX17 Mutations to the Pathogenesis of Pulmonary Arterial Hypertension
… Moreover, the effects of SOX17 mutations on protein biochemistry, namely SOX17 DNA binding capability and localisation, were also investigated in this study. WT SOX17 localised to the nucleus of both PAECs and C2 iPSC-ECs. Interestingly, C2 SOX17+/R140P iPSC-ECs exhibited nuclear SOX17 …
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Mechanistic insights into endoribonuclease activation of the mRNA 3' end processing machinery by Cleavage Factor IA
… 3' end processing is conducted by a large 14-protein complex of ~850 kDa called Cleavage and Polyadenylation Factor (CPF). CPF contains three enzymatic activities that each form the core of one of three interconnected modules: the cleavage module, the polymerase module and the phosphatase …
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Characterization of Synaptotagmin Function In Calcium Dependent Neuronal Exocytosis
Es wird vermutet, dass das Protein Synaptotagmin 1, das in der Membrane des synaptischen Vesikels sitzt, der Kalziumsensor in der neuronalen Synapse ist. Synaptotagmin 1 hat zwei sogenannte C2 Domänen, die kalziumabhängig Membranen binden. Es ist gezeigt worden, dass die Deletion von Synaptotagmin …
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Temperature- and pressure-induced protein dynamics from microseconds to minutes
For the past two decades, protein folding experiments have been speeding up from the second or millisecond time scale to the microsecond time scale, and full-atom simulations have been extended from the nanosecond to the microsecond and even millisecond time scale. Where the two meet, it is now …
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Σχεδιασμός, έκφραση και δομικές μελέτες τμημάτων του ανθρώπινου νευρωνικού νικοτινικού υποδοχέα ακετυλοχολίνης
Οι νικοτινικοί υποδοχείς ακετυλοχολίνης (nAChRs) ανήκουν στους υποδοχείς Cys-θηλιάς, που διαμεσολαβούν και ρυθμίζουν την ταχεία συναπτική νευροδιαβίβαση. Είναι πενταμερή κανάλια ιόντων που ενεργοποιούνται από προσδέτη και απαρτίζονται από πέντε ομόλογες υπομονάδες, καθεμία εκ των οποίων …
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Relationship Between TDP-43 Toxicity and Aggregation in Saccharomyces Cerevisiae
<p>Protein aggregation and inclusion body formation are hallmarks of neurodegenerative diseases such as Alzheimer's, Parkinson's, Huntington's, and amyotrophic lateral sclerosis (ALS). These neurodegenerative diseases share a common pathology in that all include accumulation of insoluble protein …
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Molecular Study of the B19 (Human) Pathogenic Parvovirus
… marrow. Replication of viral DNA and the viral protein products of B19 appear similar to those of other animal parvoviruses. However, B19 differs from other parvoviruses in some important aspects, which include the initiation of all transcripts at a strong left side promoter (p6) and the absence …
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Structural and biochemical studies of the yeast linker histone, Hho1p
… of DNA wrapped around an octamer of core histone proteins. Linker histones bind through their globular domain at the nucleosome dyad and to internucleosomal DNA through their C-terminal basic tail. The Saccharomyces cerevisiae linker histone homologue, Hho1p, contains two domains, GI and GII, that …
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