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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 46 for “"protein assembly"”.
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Protein Assembly for a Functional Fibrous Product
Natural protein-based materials are exploring their new applications from the traditional uses. Structural proteins provide scaffolds, whereas functional proteins carry essential biological activities through millions of biochemical reactions. The idea of implementing functionalities into natural …
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Models for protein assembly in the prion diseases
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 1994.
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Implications of nanoparticle-protein interactions on protein assembly and conformation
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2026-08-01
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Computational Algorithms for Predicting Membrane Protein Assembly From Angstrom to Micron Scale
… Of the wide range of barriers, the paracellular protein interfaces of epithelial and endothelial cells called tight junctions with high molecular specificities are vital for homeostasis and to maintain proper health. While the breakdown of these barriers is associated with serious pathological …
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Computational Algorithms For Predicting Membrane Protein Assembly From Angstrom To Micron Scale
… Of the wide range of barriers, the paracellular protein interfaces of epithelial and endothelial cells called tight junctions with high molecular specificities are vital for homeostasis and to maintain proper health. While the breakdown of these barriers is associated with serious pathological …
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Biomolecular function and failure across length scales
Nature has evolved proteins to form the fundamental machinery of life through controlled assembly. Such protein assembly can span five orders of magnitude in length scale, from subnanometer intramolecular arrangements to the formation of micron- sized compartments. However, our understanding of …
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Structural and biochemical characterization of nuclear pore complex structural scaffold sub-complexes
… nuclear pore complex (NPC) is a large, modular protein assembly that regulates nucleocytoplasmic transport in all eukaryotes. The ~60-120 MDa NPC is a modular assembly of multiple copies of ~30 distinct proteins that are arranged into biochemically distinct sub-complexes. We believe that the …
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Toward a Database of Geometric Interrelationships of Protein Secondary Structure Elements for De Novo Protein Design, Prediction and Analysis
… methods of analyzing, simulating, and modeling proteins are essential towards understanding protein structure and its interactions. Computational methods are easier as not all protein structures can be determined experimentally due to the inherent difficultly of working with some proteins. In …
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Spatial and temporal estimation of nanomechanical properties via Atomic Force Microscopy
… applications ranging from polymeric surfaces to protein assembly are studied using this method. In addition to uncovering fundamental surface properties, the groundwork for applying nanomechanical identification for new applications such as forensic identification for bacterial spores are also …
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Fast Protein and RNA Folding on a Rough Energy Landscape
… experimental methods, the folding process of protein assembly, protein molecules, and RNA molecules was focused in this work. For the first time, time-resolved self-assembly of a light-harvesting protein complex was studied using the laser T-jump method. Experiment results show that this …
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Physico-Chemical Characterisation of Disease-Related Protein-Protein Interactions in Solution
Current standard techniques for investigating protein-protein interactions require volumes and concentrations significantly higher than those relevant under physiological conditions. Microfluidic diffusional sizing offers an alternative method for investigating protein-protein interactions and …
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ARCHITECTURE AND RNA RECOGNITION BY THE EUKARYOTIC M6A METHYLTRANSFERASE COMPLEX
… the m6A Methyltransferase Complex (MTC), a multi-protein assembly composed of seven subunits in humans and six in Saccharomyces cerevisiae. However, the complete composition and architecture of the active MTC remain incompletely defined. This dissertation presents the in vitro reconstitution of an …
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A role for a predicted E. coli inner membrane lipoprotein in cell envelope integrity
… is composed of phospholipids, glycolipids, and proteins. A large portion of the bacterial cellular proteome is composed of membrane proteins. Despite this, the functions of several membrane proteins are as yet undefined. Therefore, defining the physiological roles of uncharacterized membrane …
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Microfluidic Techniques for Protein Detection and Manipulation
Protein-protein interactions are of central importance in biological systems. The interplay between proteins determines the functioning of such systems. Accordingly, a variety of methods have been established for the study of protein-protein interactions, but each class of methods has key …
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The Tansmembrane Associations of HIV-1 Vpu and its Human Targets
… response through the expression of accessory proteins that modulate the activity of immune system proteins. The HIV-1 protein Vpu functions to downregulate a number of host cell surface proteins within infected cells. For several target proteins, including the NTB-A and PVR receptors and the …
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Assay for Rifampicin Resistant M13 RF Synthesis
… the sequence recognized by the replication protein n' has been isolated from bacteriophage ØX174 and cloned into an Ml3 bacteriophage vector. Protein n' is a component of the bacterial primosome, a multi-protein assembly which initiates the synthesis of primers during DNA replication in the …
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Maintenance of Genomic Integrity by RSF1 Chromatin Remodeler
… 4 D. Plasmid and purification of recombinant proteins 5 E. Immunoprecipitation and in vitro binding assay 5 F. In vitro kinase assay 6 G. Cell fractionation 6 H. Chromosome spreading 7 I. Immunocytochemistry 7 J. Live cell imaging 7 III. RESULTS 9 A. RSF1 localizes at mitotic kinetochores and …
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Leveraging Protein Homeostasis as a Disease-modifying Strategy
Protein homeostasis (proteostasis) is a fundamental determinant of cellular health, and its impairment is causally linked to a wide range of human diseases. While extensive eHort has gone into characterising how the proteostasis network (PN) maintains proteins in their functional states, more work …
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MOLECULAR CHARACTERIZATION OF THE PUTATIVE NUCLEOTIDE- BINDING AND HYDROLYZING ACTIVITIES OF THE ELLIS VAN CREVELD (EVC) PROTEIN
… shown to be responsible for EvC syndrome. The protein products of these genes, EVC and EVC2 physically interact and together, they are tethered to the base of the primary cilium of cells. This multi-protein assembly positively regulates the Sonic and Indian hedgehog signaling pathways by …
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Cryo-electron microscopy studies on ovine mitochondrial complex I
… Complex I is the largest and most elaborate protein assembly of the respiratory chain with a total mass of 970 kilodaltons. It consists of 14 conserved ‘core subunits’ and 31 mitochondria-specific ‘supernumerary subunits’. Together they form a giant, Lshaped molecule, with one arm buried in …
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