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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"protein arrays"”.
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Fabricating protein arrays using Dip-pen Nanolithography techniques for improved sensitivity of biomarker detection
… methods were optimised to create PSA immunoassay arrays, which were detected by fluorescence microscopy and gave good limits of detection (LOD). Furthermore, the assay method proved adaptable, as consistent results were obtained from two different functional glass substrates. To improve the …
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Multiplex-Serologie - Simultane Analyse von Antikörper-Antworten gegen humane Papillomviren (HPV) mit Protein-Arrays
… jedoch durch die Vielfalt der Virustypen und -proteine und die Typspezifität der gegen sie gerichteten Antikörper erschwert. Der bisher zur Detektion von Antikörpern genutzte GST capture ELISA ist methodisch ausgereift und gut validiert, im Hinblick auf die Zahl parallel analysierbarer Antigene …
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Fluorescence resonance energy transfer study of the global folding of functional DNAs and electrohydrodynamic printing of protein arrays
… jet (E-jet) printing is applied to the protein microarray field. With the development of multi-nozzle printing system, both single-protein array and multiple-protein arrays are successfully demonstrated. Several proteins, such as streptavidin, Green fluorescence protein (GFP), mCherry (a …
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Development of computational methods for custom protein arrays analysis : a case study on a 100-protein ("CT100") cancer/testis antigen array
Custom antigen arrays offer a platform to assay the serological response of cancer patients to at set of selected cancer testis antigens in order to infer a diagnosis value or to assess the patient responses to particular treatments. However, the acquisition of the array data is subject to bias and …
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Engineering streptavidin and its target ligands with both infinite binding affinity and reversible binding capability
… of reusable biosensor chips, bioreactors, protein arrays, and matrices for affinity purification of proteins, it would be ideal for streptavidin to have extremely tight binding to its target ligands (biotin and its binding tags) and at the same time to retain the feature of reversible …
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Plasmachemical Functionalisation for Modification of Surfaces with Biomolecules and Metals
… the construction of covalently tethered DNA or protein arrays is examined, and novel routes are presented based on the introduction of aldehyde and bromine functions onto substrates such as glass, silicon, polystyrene beads and carbon nanotubes. Secondly, the use of plasmachemical layers for the …
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New polymeric biomaterial interfaces for biosensor applications
… resisted adsorption of antibodies or other proteins, and created a bio-inert area when patterned by POPS. On the other hand, desired proteins can be covalently attached to the graft copolymer by introducing proper coupling agents. Arrays of proteins were produced by either simple adsorption …
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Templated self-assembly for complex pattern fabrication
… block copolymer self-assembly generates dense arrays of lines or dots without long-range order. Fortunately, physical features defined by electron lithography can guide the self-assembly of block copolymer. In our previous work, the orientation of cylindrical phase block copolymer was …
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Microfluidic Devices for Bioanalytical Applications
… in fabrication and optimization of biotin-avidin protein bioassay. In order to optimize the performance of protein bioassay, various substrates and protein blocking reagents have been tested. Silicon dioxide substrate with proper thickness provide a great option for build biotin-avidin bioassay on …
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Scanning Probe Investigations of Magnetic Nanoparticles, Protein Binding and the Synthesis of Rare Earth Oxide Nanoparticles Using Nanoscale Lithography
… studies of the chemical steps for patterning proteins are critical for the integration of biomolecules into miniature biological-electronic devices for protein sensing. Rare earth oxide (REO) nanomaterials have useful properties such as upconversion, catalysis, and magnetism. For commercial …
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Micro and Nano patterns for Biosensing: from enzymatic assays to single cells interaction arrays
… for the realization of complex biological arrays. Inkjet printing techniques were employed for the generation of drug screening platforms. This approach was initially proved with a model enzyme system like Glucose Oxidase substrate covalently linked to a functionalized silicon oxide …
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Ultrasensitive Biodetection based on Plasmonically-active Materials
… conditions.1 A disease biomarker (e.g. protein, a fragment of a pro-tein, DNA/RNA, or metabolites) is a ‘molecular signature’ of the physiological state of patient at specific time and is therefore extremely important for early and possibly pre-symptomatic diagno-sis and accurate …
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Chemotactic and inflammatory responses to and recognition of Encephalitozoon spp. of microsporidia
… co-culture chemotaxis assays, limited gene and protein arrays, ELISA, and neutralizing antibody assays. These studies identified three major monocyte chemoattractants, CCL2, CCL3, and CCL4 that were upregulated, produced, and secreted in response to Encephalitozoon infections. Furthermore, these …
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An Interdisciplinary Investigation of Conformational Changes in Quasi-Crystalline Protein Array R-bodies in Response to pH
R-bodies are ribbon-like protein polymers that undergo a dramatic conformational change from a tightly coiled form at neutral pH to an extended helical spiral at acidic pH. R-bodies were found in bacterial endosymbionts of paramecia, where their forceful extension causes vacuolar membranes to …
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Integrating Functional Genomics, Proteomics and Computational Analysis for the Characterization of Cellular Networks
… found important characteristics of eukaryotic protein interaction networks that have direct implications in cellular phenomena like robustness and the efficiency of information processing. I have identified an entirely new class of functional modules with unique properties that contribute to …
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Reactive and soft landing of polyatomic gas-phase ions on plasma-treated metal surfaces
… metal surface of multiply protonated protein ions results in a substantial retention of protein function, as demonstrated for trypsin and streptavidin. The majority of trypsin ions soft-landed at hyperthermal kinetic energies retain enzymatic activity. The landed streptavidin can be …
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Discovery and Development of Predictive Biomarkers for the Personalization of Pemetrexed Therapy in Non-Small Cell Lung Cancer
… by mRNA expression profiling and reverse phase protein arrays. Furthermore, I determined that mutations in KRAS were significantly more frequent in sensitive lines, and EML4/ALK adenocarcinomas conferred sensitivity to pemetrexed, consistent with recent clinical findings. In order to test …
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Pathogenic and Protective Potential of B Cell Dysregulation in Systemic Lupus Erythematosus
… by which autoantibodies accumulate in SLE. Protein arrays can be used to identify autoantibodies and autoantigens that are targeted in SLE patients. Using this approach, we identified Stress Induced Phosphoprotein 1 (STIP1) as an autoantigen in a subset of SLE patients. Those patients with …
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Novel Methods for the Control and Detection of Protein Interactions on Surfaces
… to inhibit the non-specific adsorption of dilute protein solutions. These polymer thin films are grafted with functional ligands through succinimide ester moieties copolymerized on the polymer backbone. This general functionalization scheme is used to attach 3-methyl-thiopropyl amine to the …
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Determining protein interaction specificity of native and designed bZIP family transcription factors
Protein-protein interactions are important for almost all cellular functions. Knowing which proteins interact with one another is important for understanding protein function as well as for being able to disrupt their interactions. The basic leucine-zipper transcription factors (bZIPs) are a class …
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