Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 75 for “"protein analysis"”.
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CE-microreactor-CE-MS-MS for protein analysis
… handling and allows fast digestions of small protein samples.In this thesis work, an on-line CE-microreactor-CE-MS-MS instrument is developed that can separate proteins in a first dimension, digest them inside a pepsin-immobilized microreactor, separate peptides in a second dimension, and …
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Fabrication of Polymeric Microfluidic Devices for Protein Analysis
… flow and nonspecific adsorption of proteins. Both column efficiency and migration time reproducibility were one order of magnitude better with derivatized PMMA µCE devices compared to untreated versions. Fast, reproducible, and efficient separations of proteins and peptides were …
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Electric Field Gradient Focusing-UV Detection for Protein Analysis
… a large volume (100 micro-L) of target proteins without significant band broadening and simultaneously removing unwanted components from the sample. Two types of EFGF devices have been investigated to concentrate and separate proteins: a fiber-based EFGF device and a hydrogel-based EFGF …
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Engineering synthetic biology routes for protein analysis using microfluidic systems
… example, in medical diagnostic devices.Membrane proteins are of great importance as subjects of physiological study,drug targets in high-throughput assays for drug discovery and drug safety screening1. Microfluidic devices can be used for manipulating and analysing proteins, greatly benefiting …
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Nanoscale protein analysis utilizing capillary electrophoresis and laser-induced fluorescence detection
… capable of microlitre volume sampling and low protein concentration detection. Furthermore, one of the major trends in high throughput screening is the growing replacement of technologies that depend on radioactivity to generate a signal with those that rely on fluorescence. This trend towards …
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Digital Protein Analysis: Technologies for Protein Diagnostics and Proteomics through Single-Molecule Detection
Protein quantification tools are valuable in biological and biomedical research and in clinical applications because they enable measurements that elucidate physiological states, including disease states. Protein biomarker detection is likely to play a major role in patient health and aid in the …
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Development of Original and Notable Techniques for Protein Analysis, Native Identification, and Characterisation
… developed to characterise solutions of proteins, or proteins themselves. Proteins are the building blocks of life, and thus they are interesting to study for a deeper understanding of the mechanisms of life but also for developing diagnostic tools as well as medical therapies. The first …
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The application of mercury-containing labels in protein analysis using complementary mass spectrometric techniques
En la presente tesis doctoral se desarrollaron nuevas metodologías para el análisis cuantitativo de proteínas basadas en la introducción química de un compuesto de mercurio capaz de unirse a las cisteínas libres de estas biomoléculas. El ácido hidroximercuribenzoico tiene un alto potencial como …
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Development and Application of Next Generation Mass Spectrometry for Protein Analysis at High-Resolution
… isotopic distributions and handling all known protein modifications on a high throughput basis. Called Middle Down Proteomics, this process identified 7454 peptides from 2-20 kDa (1472 unique) from 555 proteins after just 23 LC-MS/MS injections. Along with greatly increased confidence for …
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Integrating Micro-Scale Separations to Matrix Assisted Laser Desorption and Ioniation Time of Flight Mass Spectrometry (MALDI-TOF-MS) for Protein Analysis
… mass spectrometry (MALDI TOF MS) for protein analysis. MALDI MS provides unsurpassed accurate mass measurements of intact bio-molecules, for example peptides and proteins, which in turn generate high molecular specificity enabling the identity, function and structure of these molecules …
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Top-Down Proteomics: Separation and Mass Spectral Characterization of Complex Cell Lysates From Methanosarcina Acetivorans and Human Cancer Cells
… data acquisition to be possible with top-down protein analysis. Taken together, these platforms represent the next step in increasing the utility of top-down.
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Microfluidic devices for studying early response of cytokine signaling
… with cell culture, cell stimulation, and protein analysis as a single device towards an efficient and productive cell-based assay development. In particular, this thesis demonstrates the feasibility of culturing human cancer cells in microliter-volume reactors in batch and fed-batch …
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Towards a Prime Factorization of Proteins
… the case in the richly complex field of protein analysis, whereby unique and novel insights into the structural organization of proteins can help illuminate their functional space, and in particular lead toward a factorization of the structural space into a set of motif building blocks, …
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Analyzing Vitamin D3 Supplementation and Deficiency in the Spinal Cord of Transgenic G93A Mouse Model of Amyotrophic Lateral Sclerosis
… feed) D3. At 113 d, the spinal cords underwent protein analysis. HiD females exhibited D3 toxicity, evidenced by increased oxidative damage and apoptosis and lower antioxidant capacity vs. AI. HiD males exhibited lower oxidative damage, inflammation, apoptosis and neuron damage vs. AI. DEF …
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Machine learning meets and enhances protein engineering
… pivotal role in modern science, and the study of proteins is equally vital due to their essential biological functions. In my research, I propose new machine learning models and algorithms, including novel generative models, advanced data augmentation techniques, innovative model architecture …
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Identification and Characterization of Bacterial and Bacteriophage Proteins Via Matrix-Assisted Laser Desorption Ionization (Maldi) Mass Spectrometry
… characterization and quantification of all proteins involved in a particular pathway, organelle, cell, tissue, organ or organism that can be studied in concert to provide accurate and comprehensive data about that system is called proteomics. Proteomics is formalized by combining the …
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Design of the First Analytical Platform for Automated Top Down Proteomics With quadrupole/Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
… leading to the first high-throughput proteome analysis of Methanosarcina acetivorans utilizing a custom FT mass spectrometer. It outlines a two dimensional separations platform that utilizes a 96 well plate nanospray robot to facilitate automated analysis on the mass spectrometer. This thesis …
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The role of sphingosine kinases and dihydroceramide desaturase in cell survival/apoptosis and inflammation pathways
… a mechanism involving p38 mitogen-activated protein kinase (MAPK), oxidative stress, and Mdm2 (E3 ligase) in HEK293T cells.;The polyubiquitinated forms of Degs1 acquire a 'gain of function' and activate pro-survival pathways, p38 MAPK/c-Jun N-terminal kinase (JNK), and X-box protein 1s …
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Isolation and Characterization of Bacteriophages Infecting Enterobacter cloacae to Reduce Bloater Defect in Cucumber Fermentations
… Φ115E-p2, were examined. Additionally, DNA and protein analysis were performed. Based on acquired data, Φ115E-p2 is a potential candidate for use as a biocontrol method to prevent bloater defect during cucumber fermentations. Φ107E-p1 is not a candidate based on the effectiveness data obtained. …
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