Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"protein activation"”.
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A Structural and Functional Analysis of the Human Angiotensin II Type-2 Receptor
… to determine what regions are important to G-protein activation. These studies will map the G-protein coupling regions of the human AT2 receptor and develop a wider understanding of AT2 receptor/G-protein interactions.
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Relationship Between CB1 and S1P Receptors in the Central Nervous System
… between these two systems at the level of G-protein activation, agonist-stimulated [35S]GTPγS binding and autoradiography were conducted. Autoradiographic studies were first performed to localize receptor-mediated G-protein activation in mouse brain. Coronal brain slices were processed for …
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EUKARYO: An Agent-Based, Interactive, Virtual Reality Simulation of a Eukaryotic Cell
… the interaction between the individual proteins in the cell. Using hybrid modelling, Eukaryo is able to model the interactions for a diverse range of biological processes, such as protein activation pathways and cytoskeleton self-assembly. Further to this, Eukaryo provides support for …
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The effect of CLAs on the expression of PKC isoforms and cell viability in breast cancer cell lines.
… 8% of the total number of cancer related deaths. Protein kinase C (PKC) isoforms have been shown to be strongly modulated in many androgen sensitive tumour cell lines. Increased expression of some PKC isoforms has also been linked to immortality in many cancer cell lines. Conjugated linoleic …
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Structural Basis of GPCR-Transducer Signaling
<p>G protein-coupled receptors (GPCRs) are a class of ubiquitous cell-surface receptors involved in the regulation of virtually every physiological processes. GPCRs typically signal through either G proteins or beta-arrestins (βarrs), each of which activate distinct cellular pathways that leads to …
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Exploring the molecular signatures of the ligand-receptor-Gi/o protein interactions of adenosine A1 and A3 receptors
… the hA1R/hA3R were measured at the level of the activation of individual G proteins. Being primarily coupled to the inhibitory G proteins, hA1R/hA3R showed little activation at the non-inhibitory G proteins (Gss, GsL, Gq, G11, G15, G12 and G13). It has been discovered in this part of the thesis …
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Regulation of Net1A Subcellular Localization By The Small Gtpase Rac1
<p>Activation of Rho family small G proteins is thought to be a critical event in breast cancer development and metastatic progression. Rho protein activation is stimulated by a family of enzymes known as guanine nucleotide exchange factors (Rho GEFs). The neuroepithelioma transforming gene 1 …
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Development of constrained fuzzy logic for modeling biological regulatory networks and predicting contextual therapeutic effects
Upon exposure to environmental cues, protein modifications form a complex signaling network that dictates cellular response. In this thesis, we develop methods for using continuous logic-based models to aide our understanding of these signaling networks and facilitate data interpretation. We …
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Functional Redistribution of Hippocampal Cannabinoid Cb1 Receptors in the Rat Pilocarpine Model of Acquired Epilepsy
… by alterations in CBl receptor binding and G-protein activation. These regionally selective changes were dependent on NMDA receptor activation during the initial insult of pilocarpine-induced status epilepticus (SE), and were independent of seizure suppression produced with phenobarbital …
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Modelling the endothelial cell response to fluid flow
… signal transduction, Rho GTPase localised activation and lamellipodium formation. The goal of this project is to understand how these separate facets interact and lead to a coordinated response. The flow is modelled over a 3D virtual cell, which naturally gives the force the flow exerts on …
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Mechanical unfolding of membrane proteins captured with single-molecule AFM techniques
… which serves as a model system for this class of proteins. Mechanical pulling of BetP molecules embedded into the lipid membranes resulted in a step-wise unfolding of the protein and revealed insights into its structural stability. Effect of the lipid environment, N- and C-terminal extensions on …
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Contributions to the Analysis of Biochemical Reaction-Diffusion Networks Stability, Analysis, and Numerical Solutions
… variety of processes. For instance, a simple activation mechanism studied here may be used to model the autoactivation of the effector caspase in the apoptosis pathway, the activation of the Akt/mTOR complex implicated in muscular growth, and twospecies population dynamics. In particular, we …
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Regulation of acid-stimulated H+ secretion by Pyk2 and MAPK signaling pathways in the outer medullary collecting duct
… and p38 (Thr180/Tyr182) was used to assay protein activation. To examine specificity of kinase activation and its effects, we used Pyk2 siRNA to knockdown Pyk2 expression levels, the Src kinase inhibitor PP 1 to inhibit Src phosphorylation, and the MEK inhibitor U0126 to inhibit ERK1/2 …
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Mechanisms of impaired red blood cell ATP release in older adults: implications for altered vascular control with age
… adults and that the cellular responses to Gi protein activation remained intact with age; and (iii) that systemic Rho-kinase inhibition via administration of fasudil improves the age-related impairments in vascular control and circulating ATP during systemic hypoxia and exercise, which may be …
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Receptor- G Protein Interactions in the Visual System: A Study of Structures and Mechanisms That Couple the Cytoplasmic Surface of Rhodopsin to the Nucleotide-Binding Pocket of Transducin
… photoreceptor rhodopsin and the heterotrimeric G protein transducin (Gt) initiates the vertebrate phototransduction cascade. This interaction also serves as a model system for the study of the molecular basis of related G protein-coupled receptor mediated signal transduction systems. …
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Development of PAC-1 as a privileged agent for combination chemotherapy
… cellular alterations. Small-molecule activation of procaspase-3 represents a particularly promising opportunity, based upon its determinant role in the execution of apoptosis and its widespread over-expression across many diverse cancer types. PAC-1 is a small-molecule that facilitates …
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The Cytoplasmic Domain of Membrane-type 1 Matrix Metalloproteinase is Required for its Survival-Promoting, but not its Migration-Promoting Function in MCF-7 Breast Cancer Cells
Membrane-type 1 matrix metalloproteinase (MT1-MMP) is a multifunctional protease that degrades proteins during cell migration, and influences cell survival. Both the protein localization and signal transduction capabilities of MT1-MMP depend on its cytoplasmic domain (CD), indicative of a diverse …
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Novel Insights into CB1 Receptor Signaling and the Anabolic Role of Cannabinoid Receptors in Bone
Activation of the CB1 receptor is modulated by aspartate residue D2.63176 in transmembrane helix (TMH) II. Interestingly, D2.63 does not affect the affinity for ligand binding at the CB1 receptor. Studies in class A GPCRs have suggested an ionic interaction between residues of TMHII and VII. In …
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Identification and molecular characterisation of elicitor-priming for resistance in tomato against <i>Botrytis cinerea</i>
… activity; 2) a cell-wall reinforcement through R protein activation, cellulose synthesis and PGs, PMEs and xyloglucan repression; 3) a fine-tuned potentiation of JA/JA-Ile synthesis and JA/ET/SA/ABA transcriptional regulation; 4) an induction of the lipid/fatty acid metabolism and phenylpropanoid …
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Antiapoptotische Wirkungen des Makrophagen-migrationsinhibierenden Faktors (MIF) auf das Tumorsuppressorprotein p53 bei der ROS-induzierten Apoptose
In the present thesis, intrinsic (ROS -> p53-activation / ROS -> mitochondrial apoptosis) and extrinsic (PI3K-Akt-Pathway) effects of the cytokine MIF (Macrophage Migration Inhibitory factor) have been examined and brought in connection with antiapoptotic and pro-proliferative effects. The …
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