Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 114 for “"proteasomal degradation"”.
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Herp Reduces ER Calcium Content by Proteasomal Degradation of SERCA
… membrane, thereby facilitating the ER associated degradation (ERAD) of SERCA. The proteasome recruitment and the subsequent degradation of SERCA reduce ER lumenal Ca2+ concentration and the Ca2+ release during ER stress which counteracts the activation of apoptosis. This proposal describes how to …
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From detection of proteasomal degradation to targeted control of protein depletion
… (UPS) regulates protein turnover and catalyzes degradation of misfolded or damaged proteins, thus playing an important role in maintaining protein homoeostasis in eukaryotic cells. The UPS has emerged as a drug target for diverse diseases characterized by altered protein homeostasis. However, …
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The Cancer Specific Ubiquitin Ligase MAGE-A3/6-TRIM28 Drives Tumorigenesis by Ubiquitination and Proteasomal Degradation of AMPK
… of AMPK by MAGE-A3/6-TRIM28 induces its proteasomal degradation, thereby enhancing mTOR signaling and inhibiting autophagy within cells. Through this modulation of AMPK, MAGE-A3/6 is also able to act as an oncogene, inducing anchorage independent growth and the growth of tumors in vivo. …
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Ubiquitin Mediated Regulation of NF-kappa B Signaling
… to K48-linked ubiquitination, and the subsequent proteasomal degradation of IκBalpha . Two important areas of research focusing on ubiquitin regulation of NF-κB signaling are addressed in this dissertation. The areas addressed include understanding how ubiquitinated substrates are targeted for …
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A Host Regulatory Circuit of HSV Gene Expression
… analysis, it was observed that STING facilitates proteasomal degradation of ICP0 via K11- and K48-linked ubiquitination. Furthermore, TBK1 mirrors the results of STING, independently of IFN, upon viral infection. As such, ablation of STING or TBK1 rescues viral production. Mechanistically, STING …
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Enhanced protein degradation by branched ubiquitin chains
… The anaphase-promoting complex (APC/C) catalyzes degradation of key regulators during the cell cycle causing proper transition through the cell cycle. Here, I report that the APC/C efficiently synthesizes substrate-attached branched conjugates and enhances recognition of ubiquitylated substrates …
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Investigating the role of Klhl31 in striated muscle
… prominently by targeting specific substrates for proteasomal degradation. Previous studies suggested a role for Kelch-like 31 (Klhl31) during myogenesis, as its expression is dependent on signals responsible for the induction of myogenesis in the somites and is slightly delayed compared to the …
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Role of Sub-Cellular Localization and Cellular Factors In Regulation of HIV-1 Tat Protein Stability
… is not precisely understood. In this study, degradation of HIV-1 Tat protein was studied in detail. Tat was predominantly localized to the cell nucleus and nucleolus. Tat showed a relatively short half life of (3.5 ± 0.07) h. 26S proteasomal inhibitor, MG132 rescued Tat from degradation …
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An investigation into the role of LUBAC and linear ubiquitin chains in antibody dependent intracellular neutralisation
… neutralisation (ADIN) mediates the rapid proteasomal degradation of antibody-bound viruses. It requires TRIM21, an Fc receptor and E3 ubiquitin ligase that resides in the cytosol. Binding of TRIM21 to the exposed Fc portion of an antibody that is bound to an internalised virus results in …
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On the role of SOCS proteins in leptin signalling
… for targeting associated proteins for proteasomal degradation. We identified two members of the SOCS family, CIS and SOCS2, as new interaction partners of the LR. We studied the binding modus of SOCS proteins and demonstrated that the SOCS-box is essential for receptor interaction of …
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Regulation of sterol regulatory element binding protein-1 in bovine mammary epithelial cells
… concentration of t10, c12-CLA inhibited Insig1 degradation therefore decreased SREBP1 maturation. Furthermore, immunoprecipitation (IP) confirmed that t10, c12-CLA reduced Insig1 proteasomal degradation by disrupting the interaction between Insig1 and UBX domain-containing protein 8 (Ubxd8), …
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Identifying Novel Interaction Between the E3 Ubiquitin Ligase Ring Finger Protein 20 (RNF20) and the Deubiquitinating Enzyme Ubiquitin Specific Protease 7 (USP7)
… ubiquitin chains that would mark RNF20 for proteasomal degradation. This interaction was seen within multiple cell lines, with the catalytic activity of USP7 being crucial to the stability of RNF20. These findings provide the foundation for future studies on how the regulation of RNF20 …
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Regulation Of Acute Myeloid Leukemia Cell Proliferation By E3 Ubiquitin Ligase DCAF15 Control Of Cohesin Dynamics
… neo-substrate recruitment, ubiquitination, and proteasomal degradation. However, the physiological function of DCAF15 remains unknown. Using a domain-focused genetic screening approach, we reveal DCAF15 as an acute myeloid leukemia (AML)-biased dependency. Loss of DCAF15 results in suppression …
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COMPARATIVE CELL BASED FUNCTIONAL ANALYSIS OF KEY WNT/BETA-CATENIN PATHWAY GENES AND GENE MUTATIONS
… ubiquitination of Beta-catenin and subsequent proteasomal degradation. When a Wnt-ligand binds to a Frizzled receptor, a signaling cascade displaces GSK3 Beta from its complex with Beta-catenin allowing Beta-catenin protein to accumulate and translocate to the nucleus. In the nucleus, …
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A study on PIP5Kalpha degradation by Nedd4-1-mediated ubiquitination
… ubiquitin E3 ligase, and undergoes proteasomal degradation via Nedd4-1-mediated polyubiquitination at lysine 88, which leads to reduction in PIP2 levels. Furthermore, we found that the mutation K88R enhances the inhibition of neurite outgrowth in Neuro2A cells. Our results suggest a …
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14-3-3Sigma Negatively Regulates The Stability and Subcellular Localization of Cop1
… and cytoplasmic COP1 ubiquitination and proteasomal degradation. 14-3-3σ targeted COP1 for degradation to prevent COP1-mediated p53 degradation, p53 ubiquitination, and p53 transcription repression. COP1 expression promoted cell proliferation, cell transformation, and tumor progression, …
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Studies of the anti-cancer potential of flavonoids in Human Nasopharyngeal carcinoma cells
… phase via enhanced protein phosphorylation and proteasomal degradation of cyclin D1, which is mediated by suppression of the Akt-glycogen synthase kinase-3beta (GSK-3?)-cyclin D1 signaling pathway. In the last third part of this study we examined the sensitisation effect of quercetin and …
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TREATMENT AND MODELING OF EPSTEIN-BARR VIRUS POST-TRANSPLANT LYMPHOPROLIFERATIVE DISORDER
… of a glycine-alanine rich domain that blocks proteasomal degradation. Thus to understand the expression of EBNA-1 and to evaluate the potential of targeting this viral antigen in the context of PTLD, we examined the role of glycine-alanine repeats (GAr) in EBNA-1 protein expression and further …
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The Role of Two Homologous E3 Ligases In Muscle Physiology
<p>Ubiquitin-mediated proteasomal degradation is an essential cellular function that is coordinated by three key components: E1 ubiquitin activating enzyme, E2 ubiquitin conjugating enzyme, and E3 ubiquitin ligases. There are an estimated 600 E3 ligases, some of which share high sequence homology; …
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APOBEC3 Transcriptional Regulation and HIV-1 Restriction in T Lymphocytes
… the APOBEC3 enzymes and target them for proteasomal degradation. Thus, viral progeny are mostly protected from APOBEC3 mutagenesis. Despite significant advances in understanding the mechanisms that govern APOBEC3-dependent HIV-1 restriction, as well as Vif-dependent counteraction of this …
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