Global ETD Search
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Showing 1 to 9 of 9 for “"protease treatment"”.
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H4:1 and H4:2 monoclonal antibodies discriminate between the normal and misfolded conformers of recombinant prion protein
… are available in the market are a combination of protease treatment and immunoassay. It would be highly advantageous if we can eliminate the proteinase treatment step. There are very few mAbs available in the market which do not require the PK step. In this project we tested two mAbs H4:1 and H4:2 …
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Protease Use In Ethanol Production From Dry Fractionated Corn
… We investigated the addition of a commercial protease as an alternative to exogenous nitrogen supplementation. Our goal was to understand how protease can be used more effectively in corn dry fractionation for ethanol production. We compared and evaluated the relative impacts of protease use …
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Characterization of lignin deposition in <i>Pinus taeda</i> L. cell suspension cultures
… Aqueous and organic solvent extractions and protease treatment yielded ¹³C enriched cell walls for solid-state ¹³C NMR spectroscopic analyses of the cell wall bound lignin component. Subsequently, an isolated lignin derivative from these cell walls was analyzed by solution-state ¹³C NMR …
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Purification and Characterization of the Prostate Cancer-Associated Antigen: 7E11-C5
… was sensitive to both periodate oxidation and protease treatment, indicating that the antigen was a glycoprotein; both carbohydrate and peptide were required for the antigenicity. The antigen was found to be sensitive to pH 2.0, while remaining unaffected after treatment with NaOH (pH 11.0). …
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The identification and characterisation of proteins interacting with SUMO-ubiquitin hybrid chains
… RAP80 from cell extracts after a SUMO specific proteases treatment. This SUMO specific protease treatment resulted in the loss of high molecular weight ubiquitin conjugates from RAP80 immunoprecipitated material suggesting that some RAP80 associated material is carrying SUMOylation dependent …
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Rat Lymphocyte Surface Glycoproteins Mediating Mitogenesis by Periodate or Neuraminidase Plus Galactose Oxidase
… the kinetics or magnitude of a Con A response. Treatment with the protease either before or after oxidation had the same result. Heat inactivation of the papain abrogated its inhibitory effect. Trypsin, chymotrypsin, and thermolysin were also found to inhibit oxidative mitogenesis (stimulation …
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The Molecular Anatomy of Mitochondrial Disease: Identification and Characterisation of Novel Nuclear-Encoded Mitochondrial Disease Genes
… microscopy and subcellular fractionation and protease digestion studies confirmed the intracellular localisation of COA7 to be the mitochondrial intermembrane space. Secondly, a novel homozygous recessive variant in TMCO6 (NM_018502.5: c.271C>T) was identified in a paediatric proband …
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Two thermostable hemicellulases and their effects on wood pulps
… pulps. Xylanase treated kraft pulp required a protease treatment after the hemicellulose treatment but before bleaching, in order to elicit any improvement in bleachability (~7% drop in κ relative to untreated control pulps). Mannanase treated kraft pulp showed no improvement in bleachability. …
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Studies on the Complement Receptor of a Human Lymphoblastoid Cell Line
… protein, since its activity is destroyed by proteases, and its regeneration is inhibited by cycloheximide at concentrations which inhibit protein synthesis. In an effort to identify the C3d receptor molecule(s), the lactoperoxidase-mediated iodination procedure of Hubbard and Cohn (1975a) was …