Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"protease resistance"”.
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Copper/zinc Sods of Salmonella Typhimurium: Enzymatic Properties and Correlation to Pathogenesis
… SodCI and SodCII: dimerization, tethering, and protease resistance. SodCI is a dimer and is not released from the periplasm by osmotic shock. Thus SodCI is ""tethered"" in the periplasm by some non-covalent interaction. In contrast, SodCII is monomeric and is quantitatively released by osmotic …
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Molecular Basis of Mammalian Prion Protein Misfolding
… affect the conversion process. I found that the protease resistance of PrP aggregates, a well known biochemical hallmark of PrPSc, was highly dependent on the presence of hydrophobic and negatively charged molecules. Altogether, the results provide evidence toward the idea that the formation of …
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Engineering developable miniprotein ligand scaffolds
… targets, while maintaining high stability, protease resistance, and expression. Next, we developed an active machine learning framework to improve the efficiency of screening potential framework sequences as scaffolds, through a computational-experimental feedback loop. Employing traditional …
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STRUCTURAL, FUNCTIONAL AND EVOLUTIONARY STUDIES OF ANTIMICROBIAL PEPTIDES
… but does not increase the toxicity or the protease susceptibility of the peptide. It is noteworthy that end tagging of ultra short peptides spanning 5-7 amino acids with hydrophobic amino acids enhances bactericidal activity, while preserving low toxicity and protease resistance.
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The development of stapled peptides as chemical tools to investigate activin A signalling and as antibacterial agents targeting MsbA-mediated efflux
… such as a susceptibility to degradation by proteases, the use of peptide therapeutics in the clinic has been limited. Peptide stapling, whereby a peptide is constrained into its binding conformation, presents a means by which some of the weaknesses of peptide therapeutics may be overcome. …
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Attacking Ras-driven cancers: Engineering a peptide inhibitor for Cdc42
… a smaller size, improved binding and improved protease resistance. Firstly, the Cdc42-ACK binding interface was characterised thermodynamically, producing a comprehensive dataset of the contribution of individual ACK residues to complex affinity. This information revealed regions of the ACK GBD …
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Controlled release of GLP-1 from affinity-based protein microspheres and quantitative analysis of the role of fiber length on phagocytosis and inflammatory response by macrophages
… of its amino acid sequence can confer protease resistance. Here we describe a strategy to prolong the release of active Glucagon-like peptide 1 (GLP-1) using Src homology 3 (SH3) domain protein microparticles that bind GLP-1 through affinity-mediated interactions. GLP-1 modified with …
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Isolation and Characterization of Anti-SLP Single Domain Antibodies for the Therapy of C. difficile Infection
… their high affinity, high thermal stability, and resistance to pepsin digestion. Our study provides the basis of a proof-of-principle model with which to develop specific, broadly neutralizing and intrinsically stable antibodies for the oral therapy of C. difficile infections, as an alternative to …