Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 1030 for “"protease"”.
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Characterisation of protease activated receptors
… were determined. The recently discovered human protease activated-2 receptor (hPAR-2) was studied using rabbit polyclonal antibodies generated with a multiple antigenic peptide comprising a region around the protease cleavage site. Immunocytochemistry and flow cytometry using affinity purified …
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Function and Regulation of Xylem Cysteine Protease 1 and Xylem Cysteine Protease 2 in Arabidopsis
… element-specific expression of the cysteine protease inhibitor, soyacystatin N in Arabidopsis. Our findings revealed that the absence of XCP2 expression due to T-DNA insertional mutagenesis did not affect plant growth and development in the laboratory. Soyacystatin N was an effective in vitro …
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Mechanistic studies of the ClpAP protease/
… also serves as the ATPase component of the ClpAP protease, where it is resonsible for binding specific substrates and unfolding and translocating them into its partner ClpP, the proteolytic component of the complex. We have used single molecule and traditional biochemical experiments to probe the …
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Mechanistic studies of a AAA+ protease
AAA+ proteases are present in all branches of life and responsible for the energy-dependent degradation of most cytosolic proteins. Substrates for AAA+ proteases are unfolded and translocated into a compartmental peptidase. The requirement for protein unfolding raises several questions. How easily …
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An extracellular protease from an extreme thermophile
The major extracellular protease of the Thermus-like organism Thermus T-351, has been isolated by Sephadex SP-C25 ion exchange chromatography, affinity chromatography on CBZ-D-phe-TETA-Sepharose and Sephadex G75 gel filtration chromatography. The purified enzyme, assigned the title “Caldolysin”, …
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Analysis of a trichomonas vaginalis cysteine protease
… is increasing focus on the role of cysteine proteases in Trichomonas vaginalis because of their role in virulence of other parasitic protozoa. Determining their location and function will provide insight about their role in the pathogenicity of T. vaginalis and their feasibility as a drug …
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Inquiries into the Mycobacterial Caseinolytic Protease System
… vulnerability, identified the caseinolytic protease (Clp) system, a central component of the proteostasis network, as essential and highly vulnerable in Mtb. As an attractive, emergent anti-tubercular target, a deeper mechanistic understanding of the Clp complex in vivo is instrumental in …
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Protease Activation and Inflammation in Acute Pancreatitis
… and morbidity. Activation of pancreatic proteases, the inflammatory response and impaired pancreatic circulation are pathophysiological events that are important in order for the disease to develop. There is no specific treatment for severe AP, and no useful marker for predicting the …
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The principal protease system in bovine milk
Plasmin is the principal endogenous protease in bovine milk. Distribution of overall proteolytic potential among milk fractions was determined using Coumarin Substrate as a synthetic substrate. Casein containing fractions had a higher amidolytic potential. However, preparation of casein by acid …
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Dengue virus and West Nile virus protease inhibitors
… of Dengue Virus and West Nile Virus NS2B/NS3 protease. Structure-activity relationship studies have led to the identification of a low micromolar hit, which will be used in a hit-to-lead campaign to generate lead compounds that display superior ADMET and PK characteristics.
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Nutrient Transporter targeted Delivery of HIV Protease Inhibitors
… threats to human health. Although inclusion of protease inhibitors (PIs) in HAART has greatly slowed the disease progression and improved the outcome of clinical treatment, the eradication of HIV/AIDS remains as a major medical challenge. Saquinavir (Saq) is the first anti-HIV PI approved by …
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Acute Pancreatitis. Studies on smoking and protease activation.
Background and aims: Activation of pancreatic proteases is considered to be a crucial event in the early phase of acute pancreatitis but the cause of this activation is not known. Most cases of acute pancreatitis can be attributed to either gallstone disease or alcohol abuse. However, little is …
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Cell-free formation of protease-resistant prion protein
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 1996.
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ANALYSIS OF PROTEASE ACTIVITY USING MEMBRANE- COATED BIOMIMETIC MICROSPHERES
Poisoning by Botulinum Neurotoxins (BoNTs) leads to the severe neuroparalytic illness known as botulism, which is characterized by flaccid paralysis, respiratory failure, and death. BoNTs are the most acutely toxic substances on the planet, so detection at physiologically relevant concentrations is …
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Unraveling the mechanism of ADAMTS13 resistance to protease inhibition
Background: ADAMTS13 is a metalloprotease that regulates the delicate balance between VWF multimeric length and its platelet capturing capacity. Unlike other ADAMTS and coagulation proteases, ADAMTS13 exhibits a prolonged half-life of several days as an active protease, suggesting that it is …
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