Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"pronuclear"”.
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Evaluation of techniques for the production of transgenic animals
… was used to detect transgene presence after pronuclear microinjection of mouse zygotes cultured to various stages of development. The transgene was detected in 88% of 1-cell, 88% of 2-cell, 44% of 4- cell, 40% of morula, and 29% of blastocysts. By comparison, the integration frequency for …
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Identification and Analysis of A Novel Role For The to usled-Like Kinase In Regulating Mitotic Spindle Dynamics
… at the posterior end of the embryo. After pronuclear meeting, the pronuclear-centrosome complex rotates 90° during centration to align on the anteroposterior axis followed by nuclear envelope breakdown (NEBD). However, in TLK-1-depleted P<sub>0</sub> embryos, the centrosome-pronuclear …
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Microsurgical Fertilization of Mammalian Eggs: An Assessment of Clinical Utilization
… significant difference was found in the rate of pronuclear formation following microinjection between spermatozoa differing in motility and maturational status. Spermatozoa found incapable of penetrating an egg by normal fertilization were shown to be capable of sperm decondensation and …
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Assessment of Murine Embryo Development Following Electroporation and Microinjection of a Green Fluorescent Protein DNA Construct
… transgene expression, and integration following pronuclear or cytoplasmic microinjection of condensed or linear green fluorescent protein DNA into murine embryos using electroporation. In experiment 1, the effect of embryo orientation (group or linear) within the electroporation chamber on …
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In vitro assessment of fertilization and embryo development with Bovine spermatozoa after scrotal insulation
… to determine apoptosis. In the final experiment pronuclear decondensation for presumptive zygotes was evaluated by differential interference contrast microscopy at 3 h time intervals from 6 to 18 h post in vitro insemination (hpi). Morphological evaluation of semen samples revealed a decrease (P …
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The In Vitro Transgene Expression and In Vivo Transgene Integration of Condensed DNA Injected into the Cytoplasm of Murine Zygotes
Pronuclear stage murine embryos received electrical stimulation in 5, 10, or 20 µs pulse lengths, and 0, 100, 200, 250, 300 or 400 voltages. Minimal embryo development occurred with 400 V. Irreversible electroporation occurred in embryos electroporated for 5 µs pulse length at 100 and 400 V, 10 …
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Studies on Fertilization in Vitro in Mammals
… to artificially activated eggs, normal appearing pronuclear eggs were generated. Therefore it appears that artificial sperm-egg fusion does not induce egg activation which occurs as a result of normal sperm-egg fusion. When sperm were microinjected into unfertilized eggs a large percentage of the …
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Cholesterol and egg activation
… metaphase II (MII) arrest and progressed to pronuclear, metaphase III and anaphase/telophase III stages. Eggs from fertile, wild-type mice were activated when loaded in vitro with excess cholesterol using a cholesterol/methyl-[beta]-cyclodextrin complex, phenocopying SR-BI KO oocytes.
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Organization of Human Sperm Chromosomes During Pronuclei Formation
… chromatin swelling exists during all stages of pronuclear development as detected by DNA staining in zygotes. The latter characteristic is proposed to reflect the cryptic nuclear heterogeneity in population of morphologically, mature normal sperm.</p> <p>Chromatin transformation in pronuclei was …
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Evolution of syngamy
… heritable genetic variation. Syngamy (nuclear/pronuclear fusion/association + "mixing" of chromosomes) is a lesser-understood and lesser-studied aspect of sex. I propose that syngamy is a type of cell division that evolved from meiosis. In most metazoans, diploidy is restored via a cell …
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Gene injection in the bovine: effect of time of microinjection and nuclear transfer technologies
… be obtained when injection is performed early in pronuclear formation (7% (11/161) at 11; 4% (61159) at 15; 1 % (1/165) at 19 haf; p<0.05). Experiment 2 examined the effect of microinjection of DNA into the germinal vesicle (gv) of bovine oocytes on subsequent development and detection of the …
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The role of the chromosomal passenger complex and condensin complex in meiotic chromosome cohesion and segregation
… mutants that displayed defects in fertilization, pronuclear fusion, and mitosis and DNA synthesis in the early divisions of embryogenesis. Among these mutations, one was mapped and molecularly characterized as an allele of the passenger complex component incenp.
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Nuclear Transfer With Porcine Embryonic Stem Cells
… the most efficient treatment for production of pronuclear stage cytoplasm. Results from NT experiments indicated that neither enucleated, unactivated oocytes nor enucleated, activated oocytes promoted development of NT embryos constructed from porcine ES cells beyond the four-cell stage. …
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Nuclear hallucinations: creating the vantage point of tamasha through the use of comic modes and irony in order to destabilise the authoritarian knowledge claims of Indian pro-nuclear documentaries
… voice-overs and comic appropriation of pronuclear arguments to arrive at strategies that can elicit a response from the realm of tamasha. These experiments are informed by a framework that treats film as a process that goes beyond the limits of the edited film; the sites of engagement …
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Applications of genome editing for disease modeling in mice
… models and human patients. Finally, we utilize pronuclear injection of Cas9 mRNA and guide RNA to generate mice harboring a mutation in the autism-associated gene CHD8 and investigate the underlying behavioral and molecular phenotype. These applications broadly demonstrate the potential of Cas9 …
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Ultrasound-guided transvaginal follicular aspiration to provide a source of bovine oocytes for gene microinjection
… (65%) from both sources were subjected to pronuclear-microinjection of foreign DNA while the remainder served as non-injected controls. Only control oocytes differed, with TVF A-derived oocytes developing to blastocyst more successfully than SHD oocytes (40.8% vs. 30.0olo~ p<0.05). …
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Liposome-Mediated DNA Transfer
… treated sperm and were examined for male pronuclear development. The results for three experiments indicate that 93.9% of the oocytes underwent maturation (81.7% for controls), 84.8% of the matured oocytes were penetrated (90.0% for controls) and 84.6% of the penetrated oocytes had a male …
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Control of endometrial secretion in cattle and production of transgenic swine
… estrous synchronization/superovulation schemes. Pronuclear microinjection and zygote culture in excised mouse oviducts also was assessed. Synchronization/superovulation procedures were: 1) sows observed for estrous behavior (NAT), 2) cyclic gilts synchronized with altrenogest (ALT) for 15 to 19 d …
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Bovine embryo microinjection, culture, microsurgery, and DNA analysis by the polymerase chain reaction technique
… (n=62) bovine embryos were collected for pronuclear and nuclear DNA microinjection. Approximately 70% of the embryos were injected with DNA and 30% served as controls. Injection did not affect (P>.05) mean development scores after 144 h of cultured in TCM-199 with OEC. Sixty-five (34%) of …
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The prospects for human germline genetic modification: scientific and bioethical considerations
… the tools of animal genetic modification - pronuclear microinjection, sperm-mediated gene transfer (SMGT), nuclear transfer, etc - could in principle be applied to humans. However, serious technical obstacles remain to be overcome before HGGM could be considered a practical therapeutic …
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