Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"promoter recognition"”.
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Structural and Computational Studies of RNA Polymerase
… understand the molecular details of bacterial promoter recognition and melting. In addition, our understanding is generally limited to highly conserved elements of the structure, with little focus on the bacterial lineage-specific domain insertions. Furthermore, we lack information about the …
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Expression and initial characterisation of the Plasmodium falciparum general transcription factors TFIIB and TLP
… protein (TLP) are key proteins involved in the recognition of the core promoter, and the initiation of RNAPII transcription initiation complex assembly. TFIIB stabilises DNA binding of the primary promoter recognition factor, TATA-box binding protein (TBP), and is involved in promoter …
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rRNA transcription as potential therapeutic target in triple negative breast cancers
… inhibits transcription by displacing essential promoter recognition factor SL1 thus preventing an initiation of transcription. BMH-21 inhibits elongation and induces rapid degradation of RNAP1. PMR-116 demonstrated a great potential as RNAP1 inhibitor, is characterized by low cytotoxity and very …
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Integration of Structural Methods to Characterize the Dynamics of Macromolecular Complexes
… throughout the process of uracil-specific promoter recognition with cryo-EM and x-ray crystallography. Using this structural information, we assess the energetics of DNA binding to the promoter binding pocket via molecular dynamics. Furthermore, energetic, and structural insights enable the …
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Structural Analysis of an RNA Polymerase Sigma Factor and Its Anti-sigma: sigma28/FLGM
… of transcription, where they play key roles in promoter recognition, melting, and transcriptional regulation, sigma is a dissociable subunit of RNA polymerase (RNAP), and sequence-specifically recognizes promoters, but only after associating with RNAP to form the holoenzyme. In the holoenzyme, …
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Regulation of transcription in Plasmodium falciparum, the causative agent of severe malaria: initial characterisation of PfTBP and PfTFIIA
… TLP and TFIIA, key proteins involved in core promoter recognition, the first step in RNAP-II transcription initiation complex assembly. The work provides strong evidence for the existence of two different PfTFIIA complexes containing different PfTFIIA-γ subunits. The data further demonstrate …
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Investigation of the regulatory interactions and dynamics between the circadian clock and sigma factors in the cyanobacterium Synechococcus elongatus
… sigma factors. Sigma factors are required for promoter recognition and transcription initiation in bacteria, and there are 10 different sigma factors in S. elongatus. In order to investigate the coupling of cellular processes in S. elongatus, we needed to find suitable fluorescent protein (FP) …
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Transcription regulation in Plasmodium falciparum : functional characterisation of general transcription factor IIB
… the Plasmodium genome, TATA-box and other core promoter elements that direct transcription initiation, cannot be determined with confidence in bioinformatics studies. Functional characterisation of the key protein factors involved in transcription initiation is a first important step towards the …
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Functional divergence of the RNA polymerase II transcription machinery in Plasmodium falciparum
… insight into the molecular mechanisms governing promoter recognition and the regulated assembly of the RNA polymerase II transcription initiation complex in P. falciparum, which are hitherto not understood. The work shows that P. falciparum expresses two highly divergent TBP family proteins that …
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Characterization of major virulence regulators of Erwinia amylovora
… RNA polymerase (RNAP) requires sigma factors for promoter recognition and initiation. In this study, we investigated the role of several sigma factors in regulating virulence gene expression in E.amylovora. Early studies have shown that hrp-type III secretion (T3SS) in E. amylovora is regulated by …
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Small RNAs Regulate Transcription by Interacting with Noncoding RNA Transcripts
… that short RNA duplexes designed to target the promoter region for human genes can inhibit or activate gene expression in a sequence dependent manner. The mechanism by which RNAs achieve promoter recognition has remained unclear. Sequence specific recognition could be achieved by (1) RNA …