Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 23 for “"promoter deletion"”.
-
Promoter Deletion Analysis of Xylem Cysteine Protease 2 (XCP2) in Arabidopsis thaliana
… to two independent methods of analysis: promoter reporter experiments and immunogold localization by electron microscopy. XCP2 may be involved in catalyzing the degeneration of the protoplast during the final autolytic stages of tracheary element differentiation. To this date XCP2 …
-
The Regulation of Alkaline Phosphatase during the Development of Dictyostelium
… regulatory elements within the <i>alp</i> promoter a series of 5' and internal promoter deletions were generated and fused to the <i>lacZ</i> reporter. The data from these promoter deletion constructs indicated a regulatory element within the -683 to -468 bp sequence that is required for …
-
The study of transcriptional regulation of necrotrophic effector genes ToxA and Tox3 in the wheat pathogen Parastagonospora nodorum
… Tox3, I conducted functional analyses on their promoter regions to identify the key factors for the regulation in P. nodorum. Being a necrotroph, P. nodorum tends to acquire nutrients from surrounding environment. It is therefore hypothesized that P. nodorum senses nutrients during infection to …
-
The study of transcriptional regulation of necrotrophic effector genes ToxA and Tox3 in the wheat pathogen Parastagonospora nodorum
… Tox3, I conducted functional analyses on their promoter regions to identify the key factors for the regulation in P. nodorum. Being a necrotroph, P. nodorum tends to acquire nutrients from surrounding environment. It is therefore hypothesized that P. nodorum senses nutrients during infection to …
-
MECHANISM OF TRANSCRIPTIONAL REPRESSION OF MMP-9 BY INTERFERON-β
… fibrosarcoma cells. Using reporter assays with promoter deletion constructs we show that IFN-beta's inhibitory effects require a region of the promoter between -154 and -72, which contains an AP-1 binding site. Chromatin immunoprecipitation (ChIP) studies indicate that IFN-beta increases histone …
-
Elucidation of Jasmonate-Responsive Promoter Elements in the Calmodulin-Like Gene CML39 in Arabidopsis
… JA-responsive cis-elements within the CML39 promoter. Deletion analysis of CML39 promoter fragments in planta reveals that some cis-elements respond in a tissue-specific manner. Analysis of transgenic MYC2 loss-of-function (myc2) mutants demonstrates that MYC2 – the preeminent JA-responsive …
-
DNA sequences required for efficient transcription of the estrogen regulated Xenopus laevis vitellogenin promoter
… site-specific mutagenesis to create a variety of deletions, mutations, and insertions in the 5$\sp\prime$-flanking region of the vitellogenin B1 promoter. The effects of these mutations on promoter activity were studied by using a homologous cotransfection system developed in our laboratory with …
-
The mechanism of cyanamide/MMS-induced DDI2/3 expression in Saccharomyces cerevisiae
… They share identical coding sequence and their promoter sequences are also highly conserved, which are referred as DDI2/3 in this dissertation. Subsequent studies revealed that DDI2/3 encode a cyanamide (CY) hydratases and are also highly induced by CY. Several approaches identified FZF1, …
-
Regulation der Transkription des hspA-Gens in Stigmatella aurantiaca und Analyse der Funktion des HspA-Proteins
… detected by primer extension. Analysis of the promoter proved various upstream regions to be required for maximum expression of hspA under stress conditions. To delimit the regulatory elements involved in hspA transcription that depends on heat shock, deletion / insertion mutagenesis as well as …
-
Analysis of the Transcriptional Regulation and Bidirectional Nature Of the Promoter of the p53 Tumor Suppressor Gene
… and C/EBPbeta-2, which bind to the p53 promoter and regulate p53 transcription. The results indicate that the two proteins bind to the -972/-953 region on the p53 promoter. RBP-Jkappa serves to repress p53 transcription during growth arrest, while C/EBPbeta-2 serves to enhance p53 …
-
Isolation and characterization of latex-specific promoters from Papaver somniferum L.
… have the laticifer cell type. The latex-specific promoters (LSPs) for the three genes were sequenced. The 2.5 kb DIOX promoter was fused to the reporter gene Β-glucuronidase (GUS) to characterize its expression pattern. To assess the functional sites within the DIOX promoter, deletions were made …
-
Regulation of Alternative Carbon Metabolism In Candida Albicans
… or indirect, the <em>FOX2</em> and <em>ICL1</em> promoter regions were analyzed using a combination of bioinformatics and promoter deletion analysis. To begin characterizing the <em>FOX2</em> and <em>ICL1</em> promoters, 5’ rapid amplification of cDNA ends (5’RACE) was used to identify two …
-
Regulation of SPARC Gene Expression by the Activator Protein 1 Transcription Factor
… and transactivation using the human SPARC promoter. The activity of the full-length SPARC promoter (-1409/+28) was 15-30 fold higher in c-Jun over-expressing cells compared to vector control cells. Promoter deletion analysis revealed that a region between -120 and -70 conferred c-Jun …
-
Transcriptional Regulation of kal-1 in the Nematode Caenorhabditis elegans
… <em>kal-1</em>’s expression. Since a 5.27kb promoter is sufficient to rescue loss-of-function defects, we hypothesized that most of the <em>kal-1</em> control elements are contained in this region. In order to address our hypothesis, a lineage of <em>P-kal-1-GFP</em> was produced to determine …
-
Regulation of human 5-aminolevulinate synthase 2 expression during erythroid differentiation and its role in x-linked sideroblastic anaemia.
… was employed in this study. Human ALAS2 promoter deletion studies demonstrated that the first 293bp of the proximal promoter was sufficient to enhance transcription in response to Epo induced differentiation of the J2E cells. Introns 1 and 8 exhibited Epo responsive enhancer activity with …
-
Cell type-specific regulation of the chicken tyrosinase promoter
… the present study, the chicken tyrosinase gene promoter was used to investigate possible differences in the regulation of tyrosinase expression in melanocytes and RPE cells. Transient transfection experiments were carried out in which reporter constructs, consisting oftyrosinase promoter …
-
Characterization of a potato Proteinase Inhibitor II gene whose expression in not wound-responsive
… activities in transgenic plants driven by pin2T promoter indicated that pin2T is not a wound-inducible gene. GUS activities driven by promoter deletion mutants ruled out the presence of the silencer sequences in upstream portion of the pin2T promoter that differed from pin2K. Comparison of pin2T …
-
CROSSTALK BETWEEN LYSOPHOSPATIDIC ACID (LPA) AND TRANSFORMING GROWTH FACTOR BETA (TGFβ) IN BREAST AND OVARIAN CANCER CELLS
… bp and -401 bp) present in the human LPA1 gene promoter. Deletion or point mutation of the distal TIE at around -401 bp abolished the inhibitory effect of TGFβ on the LPA1 promoter activity as revealed by luciferase assays. A DNA pull-down assay showed that the -401-TIE-E2F4/5 sequence was …
-
Expression and regulation of N-Myc Downstream- Regulated gene 1 in squamous cell carcinoma of the oesphagus
… suggesting both tumour suppressor and tumour promoter functions for NDRG1, implicating it in tumourigenic processes such as metastasis and angiogenesis. Our immunohistochemical analysis if NDRG1 expression in OSCC tissue and matched normal epithelium (n=83) showed that NDRG1 expression is …
-
Towards Identifying Cis and Trans Regulators of Expression of Xylem Cysteine Protease 1 (XCP1) in Arabidopsis
… tracheary element-specific gene expression. A deletion analysis of the XCP1 promoter was conducted to identify promoter elements that are necessary and sufficient for tracheary element-restricted gene expression. Two regions required for tracheary element-specific gene expression were …
Page 1 of 2