Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"promoter analysis"”.
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Promoter analysis in transgenic sugar beet
… which encodes P-D-glucuronidase (GUS) to examine promoters in transgenic sugar beet. GUS activities were measured in eight replicates of each of eight sugar beet hairy root clones which had been obtained after inoculation of material grown from a single seed with a CaMV 35S promoted uidA …
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Molecular characterization and promoter analysis of the maize cytosolic glyceraldehyde 3-phosphate dehydrogenase gene family
… under anaerobiosis. This study extends further analysis of the gpc gene family, with specific emphasis on gpc4. Analysis of full length cDNA clones for GAPC2, GAPC3 and GAPC4 revealed that the deduced amino acid sequence of GAPC4 has 99.4% identity with the GAPC3 cDNA clone as compared to only …
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Transcriptional Regulation of Matrix MetalloProteinase-3 in Rat Brain Astrocytes.
… by stimulation with LPS, IFN？^ and gangliosides. Promoter analysis revealed that sequences between -500 to start codon are important in induction of MMP-3. EMSA-based study and site-direct mutagenesis revealed that AP-1 is important in induction of MMP-3. Since three AP-1 binding sites (we …
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Cluster analysis of soybean pathogen-responsive genes and functional characterization in Arabidopsis
… research project provides genes to be used for promoter analysis and identifies genes specific to pathogen infection. Genes will later be transformed into soybean to determine if they enhance resistance, and if so, their sequences can be developed into molecular markers to assist breeders in …
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Investigation of Transcriptional Regulation of 5'-Nucleotidase in Dictyostelium Discoideum
… positioning during development. Southern blot analysis showed a single form of the gene. The expression of the 5nt gene is known to be developmentally regulated. The message appears first at about 5 hr of the Dictyostelium development and remains constant throughout the rest of the development. …
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Identification of the Pokeweed Antiviral Protein Interactome by Co-Immunoprecipitation-Mass Spectrometry
… co-IP. Differential expression and in silico promoter analysis demonstrated PAP and paCP1 co-expression in response to jasmonic acid, supporting the role of this interaction in defense. This work represents the first protein interactome mapping for a RIP; identification of PAP interactors in …
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Attempts toward the expression in transgenic Arabidopsis thaliana of Esi47, a salt stress-induced gene encoding a protein kinase from Lophopyrum elongatum
… transformation constructs were made for gene promoter analysis. Expression constructs were also made and tested for the production of Esi47 protein in E. coli using the pATH11 trpE fusion vector. The tissue-culture method was partially successful. Callus cultures were established for the …
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The Regulation of Alkaline Phosphatase during the Development of Dictyostelium
… regulatory elements within the <i>alp</i> promoter a series of 5' and internal promoter deletions were generated and fused to the <i>lacZ</i> reporter. The data from these promoter deletion constructs indicated a regulatory element within the -683 to -468 bp sequence that is required for …
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Identification of Regulatory Binding Sites and Corresponding Transcription Factors Involved in the Developmental Control of 5'-nucleotidase Expression in Dictyostelium discoideum
… temporally-regulated control elements within the promoter of the 5nt gene, 5â and internal promoter deletions were designed and fused to the luciferase and lacZ reporter genes, and reporter enzyme activity was measured in cells from the slug stage of development. The results from these experiments …
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A Biclustering Approach to Combinatorial Transcription Control
Combinatorial control of transcription is a well established phenomenon in the cell. Multiple transcription factors often bind to the same transcriptional control region of a gene and interact with each other to control the expression of the gene. It is thus necessary to consider the joint …
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S100A4 regulation and function
… well with integrin α6β4 expression. Using siRNA, promoter analysis and chromatin immunoprecipitation (ChIP), I demonstrate that S100A4 is regulated by NFAT5 in breast cancer cells. To study the regulation of S100A4 by NFAT5 in a more readily inducible model, I used colon cancer cells under …
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The Role of Stromal-Derived Factors in Neuroblastoma Differentiation
… transcription factor to Sp-1 sites on the TβRIII promoter. Analysis of patient microarray data demonstrate that other members of the differentiating stroma secretome, including HBEGF and EGFR, are positively correlated with TβRIII expression, suggesting that these proteins may be co-regulated. …
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Genes from Arabidopsis involved in iron-sulfur cluster biogenesis
… The results of these experiments, together with promoter analysis described in this dissertation using GUS fusions suggested that the genes encoding the AtISU scaffold proteins are regulated at the transcriptional and probably also at the posttranscriptional level. Gene silencing experiments …
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Isolation and characterization of latex-specific promoters from Papaver somniferum L.
… have the laticifer cell type. The latex-specific promoters (LSPs) for the three genes were sequenced. The 2.5 kb DIOX promoter was fused to the reporter gene Β-glucuronidase (GUS) to characterize its expression pattern. To assess the functional sites within the DIOX promoter, deletions were made …
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Influence of Insulin, Insulin-Like Growth Factor I and Sex Steroids On the Regulation of the Vitellogenin Receptor In Developing Oocytes of Largemouth Bass (Micropterus Salmoides)
… of the first vertebrate <italic>vtgr</italic> promoter revealed a number of DNA motifs that are potential targets of hormone pathways including GATA-1 and ERE, among others. Functional promoter analysis studies revealed a primary role for <italic>esr1</italic> and <italic>esr2a</italic> in …
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Identification and characterization of factors involved in DNA demethylation and anti-silencing in Arabidopsis
… In the first system, which is based on 35S promoter driven <em>SUC2</em> (sucrose transporter 2) transgene, a mutant (<em>91-1</em>) was isolated and map-based cloning identified the responsible gene as <em>MBD7</em>(methyl-CpG binding protein 7). As important interpreters of DNA …
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MtCEP1 peptides regulate lateral organ development in the model legume, Medicago truncatula
… This corroborates the results with MtCEP1 promoter analysis using GUS reporter construct (pMtCEP1:GUS) which showed high expression in lateral root primordia when grown in low nitrogen condition. These results suggest the direct regulation of MtCEP1 in regulating lateral roots. Nonetheless, …
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MtCEP1 peptides regulate lateral organ development in the model legume, Medicago truncatula
… This corroborates the results with MtCEP1 promoter analysis using GUS reporter construct (pMtCEP1:GUS) which showed high expression in lateral root primordia when grown in low nitrogen condition. These results suggest the direct regulation of MtCEP1 in regulating lateral roots. Nonetheless, …
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Physcomitrella patens (Hedw.) B.S.G. als Bioreaktor : quantitative Promotoranalyse und Produktion eines komplexen humanen Blutgerinnungsfaktors
Die vorliegende Arbeit diente der Etablierung von Physcomitrella patens (Hedw.) B.S.G. als System zur Produktion rekombinanter Proteinpharmazeutika. <br>Um ein Produktionssystem für heterologe Proteine flexibel nutzen zu können, ist es notwendig, die Expressionseffizienz der Transgene zu …
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Studies on Methylmalonyl-CoA Mutase from Escherichia coli
… a functional Sbm protein in the mutant strain. Promoter analysis indicated the presence of a promoter induced by σS, a transcription factor involved in the expression of proteins under stress or stationary phase growth conditions. Reverse transcription polymerase chain reaction (RT-PCR) studies …
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