Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"proliferative cells"”.
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Mitotic cell cycle progression and differentiation of germline stem cells in Caenorhabditis elegans
In the C. elegans germline, stem cells make a decision to retain the germline proliferative cell fate or differentiate by entering meiosis. Importantly, this decision must be coordinated with progression through the mitotic cell cycle. Previous work has shown that the conserved GLP-1/Notch …
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Exploring cancer metabolism through isotopic tracing and metabolic flux analysis
… Various types of metabolic alterations in cancer cells have been documented, prompting extensive investigation of the link between reprogrammed cell signaling pathways and rewired cellular metabolism. In addition, drug targeting of rewired metabolic pathways has been demonstrated to be a promising …
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Development of Extrachromosomal Genetic Technologies for Persistent and Tunable Expression of Therapeutic Payloads
… non-integrated transgene expression in proliferative cells over 12 weeks. Despite this ability, our EGT’s large size limits therapeutic applications. We focused on expanding carrying capacity by investigating accommodating delivery methods—dual integrase-deficient lentiviruses (IDLVs), …
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TRAIL mediated apoptosis in arthritis.
… and prolonged half life of inflammatory cells in rheumatoid arthritis (RA). A similar phenomenon is seen in malignancy. Therefore, defects in apoptosis pathways that have been proposed to contribute to the pathogenesis of malignancy may also be seen in RA. It has been reported by many …
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High-dose mode of mortality in Tribolium: A model system for study of radiation injury and repair in nonproliferative tissues
… mode of death is the result of damage to nonproliferative tissues. Relative resistance of a strain to the hyperacute or high-dose mode of death was not correlated with resistance to the midlethal mode, which is believed to be the result of damage to the proliferative cells of the midgut.
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Liver fluke development: a target for parasite control?
… Recent studies have identified a population of proliferative cells within Fasciola hepatica (Fasciola proliferative cellsFPCs) that resemble neoblast-stem cells, both morphologically and behaviourally. These cells are the only proliferating cells in Fasciola hepatica and can be localised using …
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Investigation of Microbiome Metabolites and Mitochondrial Function in a model of Non-Alcoholic Fatty Liver Disease
… bioenergetics in cell models of liver steatosis. Proliferative and differentiated HepaRG cells were cultured under standard conditions, and steatosis was established by 48h exposure to oleate and palmitate (2:1 molar ratio). Lipid accumulation was assessed by BODIPY™ staining and quantified by …
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Investigating dysregulation in protein quality control mechanisms in senescent cells
… distinct cell-states, suggesting that senescent cells do not mount a particularly dysregulated response to external stress. Senescent cells did appear to feature slightly dampened accumulation of proteins in the insoluble fraction following stress, a finding which was complemented by our results …
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Bmp proteins in urodele myotube cell cycle re-entry and in regeneration
… dedifferentiation of fully differentiated cells into proliferative cells. One well-studied example of this is the dedifferentiation of multinucleated muscle cells into mononucleate cells resembling their precursors, the myoblasts. To form these mononucleate cells the differentiated myotubes …
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Behavioral and genetic characteristics of intestinal cell lineages in health and disease
… is constantly turned over and renewed by the proliferative stem cells located at the bottom of the crypt. While this system is crucial for nutrient absorption, any derangements in the proliferative cells can quickly lead to cancer. In this thesis, we sought to better understand the behavioral …
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Identification of pluripotent stem cells and characterization of glia in the planarian Schmidtea mediterranea
… is known that neoblasts, the population of all proliferative cells in the adult planarian, are the source of new tissue during regeneration, it is unknown whether neoblasts consist of multiple subpopulations of lineage-restricted multipotent stem cells or if there exists a pluripotent stem cell …
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Telomerase Activity in Human Preimplantation Embryos
… described as an essential component of highly proliferative cells, which stabilizes the telomeres and avoids cellular senescence. Telomerase has been identified in various embryonic cell stages, hematopoietic cells, and in >85% of tumor tissue biopsies analyzed. The ability to measure the …
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Developing High Purity Embryonic Stem Cell-Derived V2a Interneurons for In Vitro Investigation and Transplantation Following Spinal Cord Injury
… (ESC-V2as) by treating differentiated cells with puromycin. ESC-V2a cultures remained free of proliferative cells, matured into normal glutamatergic neurons, and were capable of forming synapses onto motor neurons in adherent monolayer culture. For further in vitro investigation and …
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Notch activation and clone fate in intestinal homeostasis and inflammation
… regulating the proliferation of intestinal stem cells (ISCs). As a result, Notch is also an important pathway during disease such as inflammation and previous literature has reported conflicting arguments on the role of Notch during inflammation. So far there is a lack of understanding of the …
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Development of an in vitro human lung organoid system to study alveolar type 2 stem cell maintenance and dysfunction during disease
… is maintained by alveolar type 2 (hAT2) cells that have the ability to self-renew and differentiate into alveolar type 1 (hAT1) cells, a critical cell type involved in gas-exchange. Cellular damage within the alveoli can lead to severe lung dysfunction, and major alveolar defects are …
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Serum Response Factor Regulates Multiple Aspects of Central Nervous System Development
… mutant mice lacking SRF in neural progenitor cells: Srf-Nestin-cKO) exhibit striking deficits in cortical axonal projections including corticostriatal, corticospinal, and corticothalamic tracts, and they show a variable loss of the corpus callosum. Neurogenesis and interneuron specification …
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Outlining a balance-point model of homeostasis in the small intestine of broiler chickens
… post-hatch life. Chapter 4 profiled changes in proliferative cells and gene expression of various stem cell marker genes during the peri-hatch period: the last three days of embryogenesis and the first week post-hatch. The stem cell marker gene Leucine Rich Repeat Containing G Protein-Coupled …
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Using high-content subcellular proteomics to identify x-ray-induced protein trafficking
… Earth, which pose minimal threat to organisms as cells have multiple mechanisms to prevent damage or death. However, high levels of IR can overwhelm these mechanisms causing a cascade of events such as oxidative stress, DNA damage, cell cycle changes and cell death. The latter outcome is why IR …