Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"proinsulin"”.
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Chromosomal localization of a proinsulin transgene inserted with a transposon-based vector into Japanese quail, Coturnix coturnix
… ƒÝm samples, and chromosomal identification of proinsulin transgene insertions by laser microdissection and nucleotide sequence from G2 Japanese quail. The 28S rDNA was found on a macrochromosome and a microchromosome pair by primed in situ hybridization, fluorescent in situ hybridization, and …
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Somatic cell gene therapy for diabetes mellitus: engineering a surrogate B-cell
… complement of enzymes capable of processing proinsulin to mature insulin. Glucose sensitive insulin secretion was conferred to these cells by transfection with plasmids containing the human preproinsulin gene (hppI-1) and the GLUT2 gene for the glucose transporter isoform 2. Insulin secretion …
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Design, Cloning, and Expression of Rat Preptin and Alanine Analogs
… a newly discovered polypeptide found on the proinsulin-like growth factor II. The alanine scanning mutagenesis was accomplished by the use of, cloning, affinity chromatography, gel electrophoresis, gene sequencing, and mass spectra. However, due to inconclusive results, more research is …
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The endoplasmic reticulum chaperone ERdj4 is required for survival, glucose metabolism and B cell development
… ER dilation and upregulation of the UPR. Proinsulin accumulated in the ER of β-cells from ERdj4 gene trap mice consistent with our previous finding that proinsulin is a substrate for ERdj4. The insulin processing enzymes, including Pcsk1, Pcsk2 and CPE, also associated with ERdj4, …
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Recombinant Expression of Preptin Analogs for Alanine Scanning Mutagenesis of Residues 27-29
… to Asp<sup>69</sup>-Leu<sup>102</sup> of proinsulin-like growth factor E-peptide. Preptin has been isolated from pancreatic beta cells, and it has been found to exhibit both metabolic and mitogenic activities. This makes preptin an attractive candidate to treat insulin-independent diabetes …
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NEW-ONSET HYPERGLYCEMIA AFTER SARS-COV-2 INFECTION
… RNA and numerous immature insulin granules or proinsulin, suggesting altered proinsulin processing as well as beta-cell degeneration and hyperstimulation. Our findings show that COVID-19 is linked to poor glycometabolic regulation, and that these abnormalities can last even after recovery. In …
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Ricin B Chain-Insulin Fusion Protein Immunomodulation of Type 1 Diabetes
… oral tolerance therapy, the diabetes autoantigen proinsulin was linked to the non-toxic B chain of ricin—an enterocyte-binding plant lectin that possesses intrinsic cell-delivery and immunostimulatory properties. The human proinsulin (INS) ricin toxin B chain (RTB) fusion protein was expressed in …
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The Role of Sox17 in Normal and Pathological Beta Cell
… in mice with prediabetes. These mice had higher proinsulin protein content in the ER of the islet cells and dilated secretory organelles in β cells. In line with the prediabetes phenotype, these mice went on to develop additional symptoms of diabetes when placed on a high fat diet, including …
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Pancreatic peptide hormone expression in the RINm5F cell line
… confirmed that RINm5F cells contained proinsulin and that low levels of insulin and/ or proinsulin were secreted into the medium. These findings suggested that the insulin content of individual cells was at the lower limit of detection for the immunoperoxidase technique. To overcome the …
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Purification of Recombinant HIS6-GFP-Preptin Fusion Protiens
<p>Preptin, a 34-amino-acid peptide derived from proinsulin-like growth factor II, has emerged as a promising therapeutic candidate for both Type 2 diabetes mellitus and osteoporosis. Its dual functionality—stimulating insulin secretion without inducing hypoglycemia and promoting osteoblast …
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Analyse der Expression des proliferationsassoziierten Proteins P8 in Betazellen des endokrinen Pankreas
… Differenzierungsmarker, PDX-1 und Proinsulin, sowie die Fähigkeit der Betazellen, Insulin zu produzieren, während einer Überexpression von P8 analysiert. Zusammenfassend wurde ein proliferationsassoziiertes Protein, das als möglicher Transkriptionsfaktor in Betazellen des endokrinen …
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The Role of TGF-β in CTB-Insulin Regulated Human Dendritic Cell Tolerance
… B subunit fusion to autoantigen vaccines such as proinsulin (CTB-INS) down regulates dendritic cell (DC) activation and induces the synthesis of DC immunosuppressive cytokines. Recent studies of CTB-INS induction of immune tolerance in human DCs indicate that increased IDO1 biosynthesis may play …
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Immunological Characterization of Human Umbilical Cord Lining Derived Cells and their Therapeutic Application in a Diabetic Mouse Model
… therapy, CLECs transduced with a modified human proinsulin gene were transplanted intra-peritoneally into streptozotocin (STZ) induced diabetic mice, resulting in significantly lower levels of serum glucose compared to control mice. This is the first detailed study in which the immunological …
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Mechanism of Chimeric Vaccine Mediated Immune Suppression of Human Dendritic Cells
… containing the pancreatic β-cell auto-antigen proinsulin linked to a mucosal adjuvant the cholera toxin B subunit (CTB-INS). Proteomic analysis of vaccine inoculated DCs revealed strong up-regulation of the tryptophan catabolic enzyme indoleamine 2, 3-dioxygenase (IDO1) shown to be directly …
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Mechanisms by which variants in the TCF7L2 gene increase the risk of developing Type 2 diabetes
… II) of TCF7L2 that regulate β cell survival, proinsulin expression and insulin maturation. The expression of many of these TCF7L2 target genes, including T2DM associated genes/loci, correlated with TCF7L2 mRNA expression in CC, but not in CT/TT genotype carriers in human pancreatic islets. …
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Amylin, tissue hypoxia and metabolic dysregulation in diabetic heart and liver
… compensate by increasing insulin production. As proinsulin and proIAPP are co-secreted, this also results in an increase in proIAPP production. Consequently, during the hyperinsulinaemic stages of T2DM, hyperamylinaemia also occurs. Human amylin then has the propensity to form oligomers, fibrils, …
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The effectiveness of GLP-1 analogues compared to DPP-4 inhibitors for beta cell function and diabetes related complications among adults with type 2 diabetes: a systematic review and meta-analysis
… plasma connecting peptide [C-peptide] and proinsulin to insulin [PI/I] plasma concentration ratio) glycated haemoglobin (HbA1c), fasting and postprandial plasma glucose levels, diabetes related complications, and adverse drug events. Seven randomised controlled trials including 2661 …
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Phenotypic analysis of human stem cells and their differentiation potential towards pancreatic cells
… The differentiated ASCs also did not express proinsulin, amylin, and C-peptide. Therefore the insulin detected by the guinea pig antibody appears not to be genuine, and may result from the previously unreported non-specific binding of the anti-insulin antibody to a related protein. Phenotyping …
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Assessment and Treatment of Impaired Insulin- Secretion and Action in Type 2 Diabetes
Type 2 diabetes (T2D) is a disease characterised by varying degrees of defect in insulin secretion and insulin sensitivity and associated with increased morbidity and mortality. Optimal glycaemic control reduces the progression of diabetic complications. Over time, there is a steady deterioration …