Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"programmed ribosomal frameshifting"”.
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Messenger RNA Destabilization by -1 Programmed Ribosomal Frameshifting
… in viruses, previous studies have identified programmed -1 ribosomal frameshifting (-1 PRF) signals in eukaryotic genomic sequences, and suggested a role in mRNA stability. This work improves and extends the computational methods used to search for potential -1 PRF signals. It continues to …
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Multi-color visualization and quantification of single RNA translation and HIV-1 programmed ribosomal frameshifting in living cells
… imaging modality was used to investigate ribosomal frameshifting during translation. Frameshifts occur through a ribosomal +1(-2) or -1 (+2) nucleotide(s) "slip" into another frame and are implicated in both human disease and viral infections. While previous work has uncovered many …
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STRUCTURAL STUDY OF HUMAN CATENIN ΒETA-LIKE PROTEIN 1 AND DOUBLE RNA PSEUDOKNOTS IN -1 RIBOSOMAL FRAMESHIFTING
… of double pseudoknots in the regulation of -1 programmed ribosomal frameshifting in RNA viruses −1 programmed ribosomal frameshifting (PRF) is utilized by many viruses to synthesize their enzymatic and structural proteins at a defined ratio. For efficient −1 PRF, two cis-acting elements are …
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Methods of single-molecule energy landscape reconstruction with optical traps
… CCR5 mRNA has been demonstrated to stimulate -1 programmed ribosomal frameshifting and we measure its structural properties. We measure the binding energy of a GA/AG tandem mismatch, one of many mismatches with unusual properties. We use our single-molecule methods to reproduce bulk measurements …
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Super-Resolution Force Spectroscopy for Revealing Sub-nucleotide Motion in Ribosomal Frameshifting and DNA Methylation
Programmed ribosomal frameshifting plays an important role for many viruses in their protein synthesis, with well-known cases including HIV and SARS-CoV-2. Aiming for an approach to defend against such viruses, techniques including X-ray crystallography, cryo-electron microscopy (Cryo-EM) and …
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Investigating the functional roles of the viral frameshift protein 2B* in EMCV infection
… within the gene for 2B. 2B* is translated via programmed ribosomal frameshifting (PRF). Unusually, the PRF is temporally regulated, increasing in efficiency from 0% at early stages of infection to 70% by 6-8 hours post-infection (hpi), thus resulting in time-dependent translation of 2B*. …
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Ribosome profiling of porcine reproductive and respiratory syndrome virus reveals novel features of viral gene expression
… in MARC-145 cells. The PRRSV genome contains two programmed ribosomal frameshift (PRF) signals, at which conserved elements promote the backwards slippage of the ribosome by one or two nucleotides. PRRSV encodes a canonical −1 PRF site, at which frameshifting is directed by a downstream RNA …